FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3198002962> ?p ?o ?g. }

• W3198002962 abstract "Inductive signaling interactions between different cell types are a major mechanism for the further diversification of embryonic cell fates. Most blastomeres in the model chordate Ciona robusta become restricted to a single predominant fate between the 64-cell and mid-gastrula stages. The deeply stereotyped and well-characterized Ciona embryonic cell lineages allow the transcriptomic analysis of newly established cell types very early in their divergence from sibling cell states without the pseudotime inference needed in the analysis of less synchronized cell populations. This is the first ascidian study to use droplet scRNAseq with large numbers of analyzed cells as early as the 64-cell stage when major lineages such as primary notochord first become fate restricted.We identify 59 distinct cell states, including new subregions of the b-line neural lineage and the early induction of the tail tip epidermis. We find that 34 of these cell states are directly or indirectly dependent on MAPK-mediated signaling critical to early Ciona patterning. Most of the MAPK-dependent bifurcations are canalized with the signal-induced cell fate lost upon MAPK inhibition, but the posterior endoderm is unique in being transformed into a novel state expressing some but not all markers of both endoderm and muscle. Divergent gene expression between newly bifurcated sibling cell types is dominated by upregulation in the induced cell type. The Ets family transcription factor Elk1/3/4 is uniquely upregulated in nearly all the putatively direct inductions. Elk1/3/4 upregulation together with Ets transcription factor binding site enrichment analysis enables inferences about which bifurcations are directly versus indirectly controlled by MAPK signaling. We examine notochord induction in detail and find that the transition between a Zic/Ets-mediated regulatory state and a Brachyury/FoxA-mediated regulatory state is unexpectedly late. This supports a broad-hourglass model of cell fate specification in which many early tissue-specific genes are induced in parallel to key tissue-specific transcriptional regulators via the same set of transcriptional inputs." @default.
• W3198002962 created "2021-09-13" @default.
• W3198002962 creator A5030856360 @default.
• W3198002962 creator A5065583130 @default.
• W3198002962 creator A5085487477 @default.
• W3198002962 date "2021-08-31" @default.
• W3198002962 modified "2023-09-27" @default.
• W3198002962 title "Single-cell analysis of cell fate bifurcation in the chordate Ciona" @default.
• W3198002962 cites W1480363003 @default.
• W3198002962 cites W1489521580 @default.
• W3198002962 cites W1534975287 @default.
• W3198002962 cites W1547794316 @default.
• W3198002962 cites W1547999461 @default.
• W3198002962 cites W1570630661 @default.
• W3198002962 cites W1596141725 @default.
• W3198002962 cites W1919257374 @default.
• W3198002962 cites W1943355039 @default.
• W3198002962 cites W1962036314 @default.
• W3198002962 cites W1965991185 @default.
• W3198002962 cites W1967151416 @default.
• W3198002962 cites W1967187300 @default.
• W3198002962 cites W1971578941 @default.
• W3198002962 cites W1971773160 @default.
• W3198002962 cites W1976397093 @default.
• W3198002962 cites W1984883254 @default.
• W3198002962 cites W1988911185 @default.
• W3198002962 cites W1989063274 @default.
• W3198002962 cites W1989772784 @default.
• W3198002962 cites W1990773683 @default.
• W3198002962 cites W1993746009 @default.
• W3198002962 cites W1997360044 @default.
• W3198002962 cites W2001997431 @default.
• W3198002962 cites W2003264562 @default.
• W3198002962 cites W2003996484 @default.
• W3198002962 cites W2004966039 @default.
• W3198002962 cites W2007244502 @default.
• W3198002962 cites W2010564301 @default.
• W3198002962 cites W2015827420 @default.
• W3198002962 cites W2018461520 @default.
• W3198002962 cites W2034951923 @default.
• W3198002962 cites W2035157530 @default.
• W3198002962 cites W2036496205 @default.
• W3198002962 cites W2040701003 @default.
• W3198002962 cites W2050718144 @default.
• W3198002962 cites W2050860610 @default.
• W3198002962 cites W2051170713 @default.
• W3198002962 cites W2061668856 @default.
• W3198002962 cites W2066456454 @default.
• W3198002962 cites W2068065491 @default.
• W3198002962 cites W2068767807 @default.
• W3198002962 cites W2080983160 @default.
• W3198002962 cites W2081924564 @default.
• W3198002962 cites W2090535741 @default.
• W3198002962 cites W2094577119 @default.
• W3198002962 cites W2095869247 @default.
• W3198002962 cites W2096033589 @default.
• W3198002962 cites W2102212449 @default.
• W3198002962 cites W2102988868 @default.
• W3198002962 cites W2104089814 @default.
• W3198002962 cites W2107383358 @default.
• W3198002962 cites W2110114082 @default.
• W3198002962 cites W2112047378 @default.
• W3198002962 cites W2117420023 @default.
• W3198002962 cites W2117645423 @default.
• W3198002962 cites W2121737844 @default.
• W3198002962 cites W2127230771 @default.
• W3198002962 cites W2128151873 @default.
• W3198002962 cites W2129217179 @default.
• W3198002962 cites W2135937351 @default.
• W3198002962 cites W2149992227 @default.
• W3198002962 cites W2154768447 @default.
• W3198002962 cites W2155496693 @default.
• W3198002962 cites W2155652476 @default.
• W3198002962 cites W2320417950 @default.
• W3198002962 cites W2474837920 @default.
• W3198002962 cites W2475024994 @default.
• W3198002962 cites W2509957998 @default.
• W3198002962 cites W2518828632 @default.
• W3198002962 cites W2529348414 @default.
• W3198002962 cites W2557069473 @default.
• W3198002962 cites W2580989000 @default.
• W3198002962 cites W2760283324 @default.
• W3198002962 cites W2769294307 @default.
• W3198002962 cites W2770344858 @default.
• W3198002962 cites W2774307122 @default.
• W3198002962 cites W2788348358 @default.
• W3198002962 cites W2794478094 @default.
• W3198002962 cites W2794480084 @default.
• W3198002962 cites W2795331645 @default.
• W3198002962 cites W2800028441 @default.
• W3198002962 cites W2800912249 @default.
• W3198002962 cites W2890764958 @default.
• W3198002962 cites W2894845181 @default.
• W3198002962 cites W2908606430 @default.
• W3198002962 cites W2916020270 @default.
• W3198002962 cites W2949177718 @default.
• W3198002962 cites W2949493119 @default.
• W3198002962 cites W2950517843 @default.
• W3198002962 cites W2950644286 @default.
• W3198002962 cites W2951798455 @default.

• next