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• W3198132548 abstract "We have previously shown in Holothuria leucospilota the maturation-inducing role of the neurohormone relaxin-like gonad-stimulating peptide (RGP). Here, we report the induction of oocyte maturation and spawning in another holothuroid, the white teatfish, H. fuscogilva , with a species-specific recombinant RGP produced in the yeast expression system. The recombinant H. fuscogilva RGP (rwt_RGP) was bioactive in vitro , as demonstrated in germinal vesicle breakdown (GVBD) assays, and in vivo , stimulating spawning in H. fuscogilva at three doses tested (50, 100 and 150 μg/kg). The spawned gametes were viable, and here we report their development until the early juvenile stage. The rwt_RGP was also bioactive in H. scabra and in H. leucospilota . We also investigated the bioactivity of two forms of synthetic RGP, whose sequences were synthesised according to that of the H. fuscogilva . The peptides have linked B- and A-chains with one form having an amidation at the carboxy terminal of the B-chain. We observed bioactivity of both synthetic peptides, as evidenced by induction of GVBD in in vitro assays and by induction of spawning in fully grown H. leucospilota . Altogether, our results provide further evidence of the gonad-stimulating role of RGP in echinoderms. We have shown that our recombinant and synthetic RGPs are applicable among the Holothuria species, providing a reliable breeding method for the highly valuable cultured species of this class. Our findings have significance both for the survival of threatened sea cucumbers and for their sustainable aquaculture. • Active recombinant Holothuria fuscogilva RGP was produced in yeast. • Chemically synthesised RGPs were as potent as the recombinant RGP. • Recombinant and synthetic RGP stimulated spawning in 3 species of sea cucumbers." @default.
• W3198132548 created "2021-09-13" @default.
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• W3198132548 date "2022-01-01" @default.
• W3198132548 modified "2023-10-18" @default.
• W3198132548 title "Spawning induction of the high-value white teatfish sea cucumber, Holothuria fuscogilva, using recombinant relaxin-like gonad stimulating peptide (RGP)" @default.
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• W3198132548 doi "https://doi.org/10.1016/j.aquaculture.2021.737422" @default.
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