Matches in SemOpenAlex for { <https://semopenalex.org/work/W3198212462> ?p ?o ?g. }
- W3198212462 endingPage "14004" @default.
- W3198212462 startingPage "13996" @default.
- W3198212462 abstract "Abstract Despite increasing awareness of the biological impacts of long‐chain fatty acyl‐CoA esters (LCACoAs), our knowledge about the subcellular distribution and regulatory functions of these acyl‐CoA molecules is limited by a lack of methods for detecting LCACoAs in living cells. Here, we report development of a genetically encoded fluorescent sensor that enables ratiometric quantification of LCACoAs in living cells and subcellular compartments. We demonstrate how this FadR‐cpYFP fusion “LACSer sensor” undergoes LCACoA‐induced conformational changes reflected in easily detectable fluorescence responses, and show proof‐of‐concept for real‐time monitoring of LCACoAs in human cells. Subsequently, we applied LACSer in scientific studies investigating how disruption of ACSL enzymes differentially reduces cytosolic and mitochondrial LCACoA levels, and show how genetic disruption of an acyl‐CoA binding protein (ACBP) alters mitochondrial accumulation of LCACoAs." @default.
- W3198212462 created "2021-09-13" @default.
- W3198212462 creator A5016770120 @default.
- W3198212462 creator A5017774638 @default.
- W3198212462 creator A5024828045 @default.
- W3198212462 creator A5030776908 @default.
- W3198212462 creator A5037677450 @default.
- W3198212462 creator A5053115128 @default.
- W3198212462 creator A5078882004 @default.
- W3198212462 creator A5083854184 @default.
- W3198212462 creator A5090294213 @default.
- W3198212462 date "2021-05-11" @default.
- W3198212462 modified "2023-10-16" @default.
- W3198212462 title "A Ratiometric Fluorescent Biosensor Reveals Dynamic Regulation of Long‐Chain Fatty Acyl‐CoA Esters Metabolism" @default.
- W3198212462 cites W1567146796 @default.
- W3198212462 cites W1750774965 @default.
- W3198212462 cites W1763805809 @default.
- W3198212462 cites W1894322524 @default.
- W3198212462 cites W1972924731 @default.
- W3198212462 cites W1982732769 @default.
- W3198212462 cites W1984586310 @default.
- W3198212462 cites W1986813347 @default.
- W3198212462 cites W2013364831 @default.
- W3198212462 cites W2017923651 @default.
- W3198212462 cites W2020433831 @default.
- W3198212462 cites W2023500217 @default.
- W3198212462 cites W2034324831 @default.
- W3198212462 cites W2034512365 @default.
- W3198212462 cites W2036297968 @default.
- W3198212462 cites W2047114739 @default.
- W3198212462 cites W2047503781 @default.
- W3198212462 cites W2059407885 @default.
- W3198212462 cites W2060812030 @default.
- W3198212462 cites W2063060777 @default.
- W3198212462 cites W2067253037 @default.
- W3198212462 cites W2071333655 @default.
- W3198212462 cites W2078905435 @default.
- W3198212462 cites W2081591951 @default.
- W3198212462 cites W2090941304 @default.
- W3198212462 cites W2093107691 @default.
- W3198212462 cites W2096718356 @default.
- W3198212462 cites W2111993410 @default.
- W3198212462 cites W2112645157 @default.
- W3198212462 cites W2129377253 @default.
- W3198212462 cites W2136776926 @default.
- W3198212462 cites W2432789362 @default.
- W3198212462 cites W2461445560 @default.
- W3198212462 cites W2486597359 @default.
- W3198212462 cites W2486975460 @default.
- W3198212462 cites W2558238640 @default.
- W3198212462 cites W2560129407 @default.
- W3198212462 cites W2604275396 @default.
- W3198212462 cites W2777040905 @default.
- W3198212462 cites W2790705492 @default.
- W3198212462 cites W2806826548 @default.
- W3198212462 cites W2903827925 @default.
- W3198212462 cites W2942701849 @default.
- W3198212462 cites W2965511862 @default.
- W3198212462 doi "https://doi.org/10.1002/anie.202101731" @default.
- W3198212462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33837610" @default.
- W3198212462 hasPublicationYear "2021" @default.
- W3198212462 type Work @default.
- W3198212462 sameAs 3198212462 @default.
- W3198212462 citedByCount "8" @default.
- W3198212462 countsByYear W31982124622021 @default.
- W3198212462 countsByYear W31982124622022 @default.
- W3198212462 countsByYear W31982124622023 @default.
- W3198212462 crossrefType "journal-article" @default.
- W3198212462 hasAuthorship W3198212462A5016770120 @default.
- W3198212462 hasAuthorship W3198212462A5017774638 @default.
- W3198212462 hasAuthorship W3198212462A5024828045 @default.
- W3198212462 hasAuthorship W3198212462A5030776908 @default.
- W3198212462 hasAuthorship W3198212462A5037677450 @default.
- W3198212462 hasAuthorship W3198212462A5053115128 @default.
- W3198212462 hasAuthorship W3198212462A5078882004 @default.
- W3198212462 hasAuthorship W3198212462A5083854184 @default.
- W3198212462 hasAuthorship W3198212462A5090294213 @default.
- W3198212462 hasConcept C104317684 @default.
- W3198212462 hasConcept C121332964 @default.
- W3198212462 hasConcept C142613039 @default.
- W3198212462 hasConcept C181199279 @default.
- W3198212462 hasConcept C185592680 @default.
- W3198212462 hasConcept C2776879804 @default.
- W3198212462 hasConcept C2779685201 @default.
- W3198212462 hasConcept C28859421 @default.
- W3198212462 hasConcept C55493867 @default.
- W3198212462 hasConcept C62231903 @default.
- W3198212462 hasConcept C62520636 @default.
- W3198212462 hasConcept C86803240 @default.
- W3198212462 hasConcept C91881484 @default.
- W3198212462 hasConcept C95444343 @default.
- W3198212462 hasConcept C98539663 @default.
- W3198212462 hasConceptScore W3198212462C104317684 @default.
- W3198212462 hasConceptScore W3198212462C121332964 @default.
- W3198212462 hasConceptScore W3198212462C142613039 @default.
- W3198212462 hasConceptScore W3198212462C181199279 @default.
- W3198212462 hasConceptScore W3198212462C185592680 @default.
- W3198212462 hasConceptScore W3198212462C2776879804 @default.