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- W3198262359 abstract "A high performance liquid chromatography (HPLC)method for the determination of six kinds of flavonoids was established and employed to the analysis of flavonoids in cassava leaves havested at different time. The chromatographic separation was performed on a CNW Athena C18-WP column kept at room temper-atur e and acetonitrile/ acetonitrile acid/ wat er mixture were used as the mobile phase with a flow rate of 1 mL/min through gradient elution. The injection volume of the sample was 5μL. The detection wavelengths were set at 281 nm for catechin and hesperidin, 338 nm for amentoflavone, 360 nm for rutin and kaempferol and 367 nm for quercetin. The linear ranges of catechin, rutin, hesperidin, quercetin, kaempferol and amentoflavone were 1.5 mg/L-194.6 mg/L(R2=0.9999),7.8 mg/L-992.6 mg/L(R2=1.0000),0.7 mg/L-84 mg/L(R2=0.9999), 0.7 mg/L-93.8 mg/L(R2=0.9999), 0.8 mg/L-108 mg/L(R2=0.9958), 0.5 mg/L-67.2 mg/L(R2=0.9995), respectively. This method was precise and accurate(RSD<3％) and the average recoveries ranged from 95.50％to 100.01％, suitable for the precise quantification of the six flavoinds in cassava leaves. The results indicat-ed that rutin and amentoflavone were the major components with the total amounts over 6 times of other 4 flavoinds. The optimal harvest time to obtain the highest amouts for catechin, rutin, hesperidin, and quercetin was 180 d after planting, 120 d for amentoflavone, and it depend on cassava varieties for kaempferol." @default.
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- W3198262359 date "2017-01-01" @default.
- W3198262359 modified "2023-09-24" @default.
- W3198262359 title "Simultaneous Determination of 6 Flavonoids in Cassava Leaves from Different Harvest Time by HPLC" @default.
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