FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3198327945> ?p ?o ?g. }

• W3198327945 abstract "Rickettsia conorii is a Gram-negative, cytosolic intracellular bacterium that has classically been investigated in terms of endothelial cell infection. However, R. conorii and other human pathogenic Rickettsia species have evolved mechanisms to grow in various cell types, including macrophages, during mammalian infection. During infection of these phagocytes, R. conorii shifts the host cell's overall metabolism towards an anti-inflammatory M2 response, metabolically defined by an increase in host lipid metabolism and oxidative phosphorylation. Lipid metabolism has more recently been identified as a key regulator of host homeostasis through modulation of immune signalling and metabolism. Intracellular pathogens have adapted mechanisms of hijacking host metabolic pathways including host lipid catabolic pathways for various functions required for growth and survival. In the present study, we hypothesised that alterations of host lipid droplets initiated by lipid catabolic pathways during R. conorii infection is important for bacterial survival in macrophages. Herein, we determined that host lipid droplet modulation is initiated early during R. conorii infection, and these alterations rely on active bacteria and lipid catabolic pathways. We also find that these lipid catabolic pathways are essential for efficient bacterial survival. Unlike the mechanisms used by other intracellular pathogens, the catabolism of lipid droplets induced by R. conorii infection is independent of upstream host peroxisome proliferator-activated receptor-alpha (PPARα) signalling. Inhibition of PPARɣ signalling and lipid droplet accumulation in host cells cause a significant decrease in R. conorii survival suggesting a negative correlation with lipid droplet production and R. conorii survival. Together, these results strongly suggest that the modulation of lipid droplets in macrophage cells infected by R. conorii is an important and underappreciated aspect of the infection process. TAKE AWAYS: Host lipid droplets are differentially altered in early and replicative stages of THP-1 macrophage infection with R. conorii. Lipid droplet alterations are initiated in a bacterial-dependent manner and do not require host peroxisome proliferator-activated receptors α or ɣ activation. Pharmacological inhibition of host lipid catabolic processes during R. conorii infection indicates a requirement of lipid catabolism for bacterial survival and initiation of lipid droplet modulation. A significant increase in host lipid droplets during infection has a negative impact on R. conorii survival in THP-1 macrophages." @default.
• W3198327945 created "2021-09-13" @default.
• W3198327945 creator A5028677558 @default.
• W3198327945 creator A5039385786 @default.
• W3198327945 creator A5064257372 @default.
• W3198327945 date "2021-09-13" @default.
• W3198327945 modified "2023-09-26" @default.
• W3198327945 title "<scp> <i>Rickettsia conorii</i> </scp> survival in <scp>THP</scp> ‐1 macrophages involves host lipid droplet alterations and active rickettsial protein production" @default.
• W3198327945 cites W1559079979 @default.
• W3198327945 cites W1648506109 @default.
• W3198327945 cites W1947112226 @default.
• W3198327945 cites W1965792495 @default.
• W3198327945 cites W1967545780 @default.
• W3198327945 cites W1978442399 @default.
• W3198327945 cites W1983006621 @default.
• W3198327945 cites W1992149604 @default.
• W3198327945 cites W2005176261 @default.
• W3198327945 cites W2007505836 @default.
• W3198327945 cites W2020516194 @default.
• W3198327945 cites W2022447085 @default.
• W3198327945 cites W2030843949 @default.
• W3198327945 cites W2031277784 @default.
• W3198327945 cites W2033541022 @default.
• W3198327945 cites W2039400507 @default.
• W3198327945 cites W2041022034 @default.
• W3198327945 cites W2041639382 @default.
• W3198327945 cites W2042207589 @default.
• W3198327945 cites W2057967679 @default.
• W3198327945 cites W2058507546 @default.
• W3198327945 cites W2059655994 @default.
• W3198327945 cites W2061300717 @default.
• W3198327945 cites W2061357909 @default.
• W3198327945 cites W2064380139 @default.
• W3198327945 cites W2073287270 @default.
• W3198327945 cites W2080674179 @default.
• W3198327945 cites W2092424177 @default.
• W3198327945 cites W2102273841 @default.
• W3198327945 cites W2103221598 @default.
• W3198327945 cites W2105389175 @default.
• W3198327945 cites W2105682847 @default.
• W3198327945 cites W2106794605 @default.
• W3198327945 cites W2111518068 @default.
• W3198327945 cites W2113702016 @default.
• W3198327945 cites W2115572749 @default.
• W3198327945 cites W2122045544 @default.
• W3198327945 cites W2123177387 @default.
• W3198327945 cites W2123527762 @default.
• W3198327945 cites W2123950558 @default.
• W3198327945 cites W2126043487 @default.
• W3198327945 cites W2127911794 @default.
• W3198327945 cites W2130639286 @default.
• W3198327945 cites W2133847815 @default.
• W3198327945 cites W2138144134 @default.
• W3198327945 cites W2139046356 @default.
• W3198327945 cites W2142610059 @default.
• W3198327945 cites W2155593866 @default.
• W3198327945 cites W2168448346 @default.
• W3198327945 cites W2169801025 @default.
• W3198327945 cites W2181027469 @default.
• W3198327945 cites W2238275403 @default.
• W3198327945 cites W2263746595 @default.
• W3198327945 cites W2328689582 @default.
• W3198327945 cites W2340680032 @default.
• W3198327945 cites W2397123170 @default.
• W3198327945 cites W2403668797 @default.
• W3198327945 cites W2417704720 @default.
• W3198327945 cites W2490472137 @default.
• W3198327945 cites W2494222081 @default.
• W3198327945 cites W2528897178 @default.
• W3198327945 cites W2588656478 @default.
• W3198327945 cites W2593417284 @default.
• W3198327945 cites W2604197619 @default.
• W3198327945 cites W2613882318 @default.
• W3198327945 cites W2621384711 @default.
• W3198327945 cites W2758211565 @default.
• W3198327945 cites W2765835620 @default.
• W3198327945 cites W2767943707 @default.
• W3198327945 cites W2782282127 @default.
• W3198327945 cites W2791421665 @default.
• W3198327945 cites W2807899982 @default.
• W3198327945 cites W2809320065 @default.
• W3198327945 cites W2810749981 @default.
• W3198327945 cites W2884600581 @default.
• W3198327945 cites W2903107073 @default.
• W3198327945 cites W2921162481 @default.
• W3198327945 cites W2924278348 @default.
• W3198327945 cites W2937029337 @default.
• W3198327945 cites W2938446982 @default.
• W3198327945 cites W2941239879 @default.
• W3198327945 cites W2961328380 @default.
• W3198327945 cites W2979586864 @default.
• W3198327945 cites W2995342721 @default.
• W3198327945 cites W2997171704 @default.
• W3198327945 cites W3003446445 @default.
• W3198327945 cites W3007131890 @default.
• W3198327945 cites W3038445261 @default.
• W3198327945 cites W3040989158 @default.
• W3198327945 cites W3041712839 @default.
• W3198327945 cites W3044160643 @default.
• W3198327945 cites W3090036866 @default.

• next