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• W3198379875 startingPage "113" @default.
• W3198379875 abstract "Glutamate decarboxylase B (GadB) from Escherichia coli, an intrinsic pyridoxal 5′-phosphate (PLP)-dependent enzyme has been employed for 4-aminobutyric acid (GABA) biosynthesis, which involves the glutamate import and GABA export via a transporter located in the inner membrane as rate determined step of whole-cell (WC) biotransformation. Herein, GadB was cloned and overexpressed in E. coli under a constitutive promoter in a high copy number plasmid, and 46.9 g/L GABA was produced. It was observed that GadB migrated to the periplasm when the WC were subjected to −20 °C cold treatment for 24 h prior to the biotransformation. Kinetic studies indicated that the enzymatic turnover rate of WC increased 2-fold after cold treatment, which was correlated with the migration rate of GadB, and up to 88.6% of GadB. The export or possible migration of GadB mitigated the rate-limiting step of WC biotransformation, and a 100% conversion of substrate to GABA was obtained. Finally, we launched a promising strategy for GABA production of 850 g/L from cost-effective monosodium glutamate (MSG) by using WC biocatalysts with 10-times recycling." @default.
• W3198379875 created "2021-09-13" @default.
• W3198379875 creator A5046514018 @default.
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• W3198379875 date "2021-11-01" @default.
• W3198379875 modified "2023-09-25" @default.
• W3198379875 title "Migration of glutamate decarboxylase by cold treatment on whole-cell biocatalyst triggered activity for 4-aminobutyric acid production in engineering Escherichia coli" @default.
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• W3198379875 doi "https://doi.org/10.1016/j.ijbiomac.2021.08.166" @default.
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