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- W3198406721 abstract "Abstract BackgroundThe Cre- lox P system is widely applied for conditional knockout mice, commonly used to study the function of specific genes. Although some different promoters drive Cre expression, the poly(I: C)-inducible Mx1-Cre is the most commonly used to delete the target gene in experimental hematology. However, the optimal induction knockout condition for Mx1-Cre/ Cre-loxP mice using the Poly(I:C)-inducible Cre- lox P conditional system remains unclear. Here, we present two different components and three injection protocols of poly(I: C) to find the optimized condition. ResultsThe results showed that the better knockout efficiency of Cre- lox P in mice injected with pure poly(I: C) has than those injected with poly(I: C) with some components. From the perspective of lethal genes ( Brg1 ), data showed that mice injected with a single high dose (500 µg) of pure Poly (I:C) had a lower knockout rate. For mice injected media-dose (10µg/g) poly(I: C) triple, which induced a high knockout rate, but the mortality rate was still high. Importantly, the mice injected low-dose (6µg/g) poly(I: C) triple, both the knockout rate and survival rate of mice was high. Similarly, the knockout rate of non-lethal mice injected with media-dose (10µg/g) or low-dose (6μg/g) poly(I: C) triple was very high, but injected with a single high dose (500 µg) of pure poly(I: C) had a low knockout rate. ConclusionOur studies provided the optimized condition for using poly(I: C)-inducible effective knockout and maintaining the survival rate for the Cre- lox P mice, which might be applied in other knockout mice for this system to ensure both the gene knockout and the mice survival." @default.
- W3198406721 created "2021-09-13" @default.
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- W3198406721 date "2021-09-09" @default.
- W3198406721 modified "2023-10-16" @default.
- W3198406721 title "The Optimal Condition of Poly(I: C)-Induced Knockout of Cre Recombinase for Mx1-Cre/ Cre-loxP Mice" @default.
- W3198406721 doi "https://doi.org/10.21203/rs.3.rs-789451/v1" @default.
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