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• W3198526188 abstract "Lignocellulose biomass has been investigated as a feedstock for second generation biofuels and other value-added products. Some of the processes for biofuel production utilize cellulases and hemicellulases to convert the lignocellulosic biomass into a range of soluble sugars before fermentation with microorganisms such as yeast Saccharomyces cerevisiae. One of these sugars is L-arabinose, which cannot be utilized naturally by yeast. The first step in L-arabinose catabolism is its transport into the cells, and yeast lacks a specific transporter, which could perform this task.We identified Trire2_104072 of Trichoderma reesei as a potential L-arabinose transporter based on its expression profile. This transporter was described already in 2007 as D-xylose transporter XLT1. Electrophysiology experiments with Xenopus laevis oocytes and heterologous expression in yeast revealed that Trire2_104072 is a high-affinity L-arabinose symporter with a Km value in the range of [Formula: see text] 0.1-0.2 mM. It can also transport D-xylose but with low affinity (Km [Formula: see text] 9 mM). In yeast, L-arabinose transport was inhibited slightly by D-xylose but not by D-glucose in an assay with fivefold excess of the inhibiting sugar. Comparison with known L-arabinose transporters revealed that the expression of Trire2_104072 enabled yeast to uptake L-arabinose at the highest rate in conditions with low extracellular L-arabinose concentration. Despite the high specificity of Trire2_104072 for L-arabinose, the growth of its T. reesei deletion mutant was only affected at low L-arabinose concentrations.Due to its high affinity for L-arabinose and low inhibition by D-glucose or D-xylose, Trire2_104072 could serve as a good candidate for improving the existing pentose-utilizing yeast strains. The discovery of a highly specific L-arabinose transporter also adds to our knowledge of the primary metabolism of T. reesei. The phenotype of the deletion strain suggests the involvement of other transporters in L-arabinose transport in this species." @default.
• W3198526188 created "2021-09-13" @default.
• W3198526188 creator A5011242246 @default.
• W3198526188 creator A5034695858 @default.
• W3198526188 creator A5036970079 @default.
• W3198526188 creator A5063603955 @default.
• W3198526188 creator A5078842111 @default.
• W3198526188 date "2021-09-08" @default.
• W3198526188 modified "2023-10-17" @default.
• W3198526188 title "Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei" @default.
• W3198526188 cites W1495464089 @default.
• W3198526188 cites W1497947649 @default.
• W3198526188 cites W1509987183 @default.
• W3198526188 cites W1519440922 @default.
• W3198526188 cites W1532574207 @default.
• W3198526188 cites W1564184122 @default.
• W3198526188 cites W1778166161 @default.
• W3198526188 cites W1936378126 @default.
• W3198526188 cites W1953352723 @default.
• W3198526188 cites W1965542198 @default.
• W3198526188 cites W1982780351 @default.
• W3198526188 cites W1983455010 @default.
• W3198526188 cites W1984354326 @default.
• W3198526188 cites W1991102251 @default.
• W3198526188 cites W1995683339 @default.
• W3198526188 cites W1997116981 @default.
• W3198526188 cites W199830504 @default.
• W3198526188 cites W1999491245 @default.
• W3198526188 cites W1999612039 @default.
• W3198526188 cites W2000350633 @default.
• W3198526188 cites W2006085890 @default.
• W3198526188 cites W2006732097 @default.
• W3198526188 cites W2010405804 @default.
• W3198526188 cites W2017519756 @default.
• W3198526188 cites W2019942659 @default.
• W3198526188 cites W2020541351 @default.
• W3198526188 cites W2025494236 @default.
• W3198526188 cites W2026589666 @default.
• W3198526188 cites W2030385177 @default.
• W3198526188 cites W2031674915 @default.
• W3198526188 cites W2039650489 @default.
• W3198526188 cites W2041117835 @default.
• W3198526188 cites W2042617105 @default.
• W3198526188 cites W2045816070 @default.
• W3198526188 cites W2051296916 @default.
• W3198526188 cites W2056775370 @default.
• W3198526188 cites W2059464705 @default.
• W3198526188 cites W2065207118 @default.
• W3198526188 cites W2068139519 @default.
• W3198526188 cites W2068954041 @default.
• W3198526188 cites W2080553628 @default.
• W3198526188 cites W2087752615 @default.
• W3198526188 cites W2093568822 @default.
• W3198526188 cites W2099456413 @default.
• W3198526188 cites W2107277218 @default.
• W3198526188 cites W2112504441 @default.
• W3198526188 cites W2117052585 @default.
• W3198526188 cites W2118069954 @default.
• W3198526188 cites W2120763174 @default.
• W3198526188 cites W2122929486 @default.
• W3198526188 cites W2124088653 @default.
• W3198526188 cites W2124494829 @default.
• W3198526188 cites W2133469996 @default.
• W3198526188 cites W2136527387 @default.
• W3198526188 cites W2145670993 @default.
• W3198526188 cites W2150853746 @default.
• W3198526188 cites W2152285267 @default.
• W3198526188 cites W2152770371 @default.
• W3198526188 cites W2152808252 @default.
• W3198526188 cites W2154617536 @default.
• W3198526188 cites W2156426990 @default.
• W3198526188 cites W2162159276 @default.
• W3198526188 cites W2162595238 @default.
• W3198526188 cites W2167387687 @default.
• W3198526188 cites W2169333620 @default.
• W3198526188 cites W2192080449 @default.
• W3198526188 cites W2225634001 @default.
• W3198526188 cites W2277123861 @default.
• W3198526188 cites W2279626864 @default.
• W3198526188 cites W2280691384 @default.
• W3198526188 cites W2285983630 @default.
• W3198526188 cites W2319486179 @default.
• W3198526188 cites W2320098481 @default.
• W3198526188 cites W2339485220 @default.
• W3198526188 cites W2479253297 @default.
• W3198526188 cites W2562555631 @default.
• W3198526188 cites W2571966499 @default.
• W3198526188 cites W2598272286 @default.
• W3198526188 cites W2613787240 @default.
• W3198526188 cites W2624753255 @default.
• W3198526188 cites W2624877708 @default.
• W3198526188 cites W2625419514 @default.
• W3198526188 cites W2738165251 @default.
• W3198526188 cites W2775435185 @default.
• W3198526188 cites W2783230082 @default.
• W3198526188 cites W2793721954 @default.
• W3198526188 cites W2796442051 @default.
• W3198526188 cites W2799524357 @default.
• W3198526188 cites W2806348293 @default.
• W3198526188 cites W2806456736 @default.

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