FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3198544462> ?p ?o ?g. }

• W3198544462 endingPage "e12025" @default.
• W3198544462 startingPage "e12025" @default.
• W3198544462 abstract "Pogostemon cablin , a medicinally and economically important perennial herb, is cultivated around the world due to its medicinal and aromatic properties. Different P. cablin cultivars exhibit different morphological traits and patchouli oil components and contents (especially patchouli alcohol (PA) and pogostone (PO)). According to the signature constituent of the leaf, P. cablin was classified into two different chemotypes, including PA-type and PO-type. To better understand the molecular mechanisms of PA biosynthesis, the transcriptomes of Chinese-cultivated P. cablin cv. PA-type “Nanxiang” (NX) and PO-type “Paixiang” (PX) were analyzed and compared with ribonucleic acid sequencing (RNA-Seq) technology. We obtained a total of 36.83 G clean bases from the two chemotypes, compared them with seven databases and revealed 45,394 annotated unigenes. Thirty-six candidate unigenes participating in the biosynthesis of PA were found in the P. cablin transcriptomes. Overall, 8,390 differentially expressed unigenes were identified between the chemotypes, including 2,467 upregulated and 5,923 downregulated unigenes. Furthermore, six and nine differentially expressed genes (DEGs) were mapped to the terpenoid backbone biosynthetic and sesquiterpenoid and triterpenoid biosynthetic pathways, respectively. One key sesquiterpene synthase gene involved in the sesquiterpenoid and triterpenoid biosynthetic pathways, encoding patchoulol synthase variant 1, was significantly upregulated in NX. Additionally, GC-MS analysis of the two chemotypes in this study showed that the content of PA in NX was significantly higher than that of PX, while the content of PO showed the opposite phenotype. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the DEG expression tendency was consistent with the transcriptome sequencing results. Overall, 23 AP2/ERF, 13 bHLH, 11 MYB, 11 NAC, three Trihelix, 10 WRKY and three bZIP genes that were differentially expressed may act as regulators of terpenoid biosynthesis. Altogether, 8,314 SSRs were recognized within 6,825 unigenes, with a distribution frequency of 18.32%, among which 1,202 unigenes contained more than one SSR. The transcriptomic characteristics of the two P. cablin chemotypes are comprehensively reported in this study, and these results will contribute to a better understanding of the molecular mechanism of PA biosynthesis. Our transcriptome data also provide a valuable genetic resource for further studies on P. cablin ." @default.
• W3198544462 created "2021-09-13" @default.
• W3198544462 creator A5000029029 @default.
• W3198544462 creator A5004206357 @default.
• W3198544462 creator A5022109528 @default.
• W3198544462 creator A5044451236 @default.
• W3198544462 creator A5054354998 @default.
• W3198544462 creator A5063103866 @default.
• W3198544462 creator A5064378508 @default.
• W3198544462 creator A5067937579 @default.
• W3198544462 creator A5088259981 @default.
• W3198544462 date "2021-08-26" @default.
• W3198544462 modified "2023-10-12" @default.
• W3198544462 title "Transcriptome analysis of two <i>Pogostemon cablin</i> chemotypes reveals genes related to patchouli alcohol biosynthesis" @default.
• W3198544462 cites W1603371265 @default.
• W3198544462 cites W1719348349 @default.
• W3198544462 cites W1849913058 @default.
• W3198544462 cites W1981509058 @default.
• W3198544462 cites W1982677301 @default.
• W3198544462 cites W1992362149 @default.
• W3198544462 cites W2008849054 @default.
• W3198544462 cites W2015543339 @default.
• W3198544462 cites W2018978047 @default.
• W3198544462 cites W2020157885 @default.
• W3198544462 cites W2020914224 @default.
• W3198544462 cites W2026688176 @default.
• W3198544462 cites W2037345633 @default.
• W3198544462 cites W2042244538 @default.
• W3198544462 cites W2042700678 @default.
• W3198544462 cites W2045204781 @default.
• W3198544462 cites W2050000402 @default.
• W3198544462 cites W2074246653 @default.
• W3198544462 cites W2076206780 @default.
• W3198544462 cites W2102242228 @default.
• W3198544462 cites W2102652793 @default.
• W3198544462 cites W2105106534 @default.
• W3198544462 cites W2107060225 @default.
• W3198544462 cites W2107277218 @default.
• W3198544462 cites W2122221811 @default.
• W3198544462 cites W2126419817 @default.
• W3198544462 cites W2131271579 @default.
• W3198544462 cites W2141458291 @default.
• W3198544462 cites W2154098874 @default.
• W3198544462 cites W2165463056 @default.
• W3198544462 cites W2170551349 @default.
• W3198544462 cites W2237696151 @default.
• W3198544462 cites W2279195969 @default.
• W3198544462 cites W2285119527 @default.
• W3198544462 cites W2293248191 @default.
• W3198544462 cites W2315880326 @default.
• W3198544462 cites W2359001286 @default.
• W3198544462 cites W2395669730 @default.
• W3198544462 cites W2470234849 @default.
• W3198544462 cites W2499393440 @default.
• W3198544462 cites W2529592713 @default.
• W3198544462 cites W2554465582 @default.
• W3198544462 cites W2587964265 @default.
• W3198544462 cites W2614235426 @default.
• W3198544462 cites W2626617466 @default.
• W3198544462 cites W2725898198 @default.
• W3198544462 cites W2737741798 @default.
• W3198544462 cites W2771985191 @default.
• W3198544462 cites W2773858386 @default.
• W3198544462 cites W277432128 @default.
• W3198544462 cites W2778476900 @default.
• W3198544462 cites W2781766060 @default.
• W3198544462 cites W2783460538 @default.
• W3198544462 cites W2789538513 @default.
• W3198544462 cites W2792405200 @default.
• W3198544462 cites W2799538853 @default.
• W3198544462 cites W2884492270 @default.
• W3198544462 cites W2884959727 @default.
• W3198544462 cites W2884985177 @default.
• W3198544462 cites W2894132562 @default.
• W3198544462 cites W2900925768 @default.
• W3198544462 cites W2904307225 @default.
• W3198544462 cites W2910730182 @default.
• W3198544462 cites W2940379058 @default.
• W3198544462 cites W2952741275 @default.
• W3198544462 cites W2954186407 @default.
• W3198544462 cites W2955998151 @default.
• W3198544462 cites W2961534454 @default.
• W3198544462 cites W2962329098 @default.
• W3198544462 cites W2990812028 @default.
• W3198544462 cites W3016902963 @default.
• W3198544462 cites W3021942802 @default.
• W3198544462 cites W3036750951 @default.
• W3198544462 cites W3037772387 @default.
• W3198544462 cites W3114122564 @default.
• W3198544462 cites W3118608459 @default.
• W3198544462 cites W3121964512 @default.
• W3198544462 cites W4211246989 @default.
• W3198544462 cites W4294216483 @default.
• W3198544462 cites W821124826 @default.
• W3198544462 doi "https://doi.org/10.7717/peerj.12025" @default.
• W3198544462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8403477" @default.
• W3198544462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34527441" @default.
• W3198544462 hasPublicationYear "2021" @default.

• next