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- W3198978829 abstract "Our therapeutic arsenal against viruses is very limited and the current pandemic of SARS-CoV-2 highlights the critical need for effective antivirals against emerging coronaviruses. Cellular assays allowing a precise quantification of viral replication in high-throughput experimental settings are essential to the screening of chemical libraries and the selection of best antiviral chemical structures. To develop a reporting system for SARS-CoV-2 infection, we generated cell lines expressing a firefly luciferase maintained in an inactive form by a consensus cleavage site for the viral protease 3CLPro of coronaviruses, so that the luminescent biosensor is turned on upon 3CLPro expression or SARS-CoV-2 infection. This cellular assay was used to screen a metabolism-oriented library of 492 compounds to identify metabolic vulnerabilities of coronaviruses for developing innovative therapeutic strategies. In agreement with recent reports, inhibitors of pyrimidine biosynthesis were found to prevent SARS-CoV-2 replication. Among the top hits, we also identified the NADPH oxidase (NOX) inhibitor Setanaxib. The anti-SARS-CoV-2 activity of Setanaxib was further confirmed using ACE2-expressing human pulmonary cells Beas2B as well as human primary nasal epithelial cells. Altogether, these results validate our cell-based functional assay and the interest of screening libraries of different origins to identify inhibitors of SARS-CoV-2 for drug repurposing or development." @default.
- W3198978829 created "2021-09-27" @default.
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- W3198978829 date "2021-09-12" @default.
- W3198978829 modified "2023-10-01" @default.
- W3198978829 title "A Bioluminescent 3CLPro Activity Assay to Monitor SARS-CoV-2 Replication and Identify Inhibitors" @default.
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- W3198978829 cites W2039910403 @default.
- W3198978829 cites W2050313712 @default.
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- W3198978829 cites W2750491563 @default.
- W3198978829 cites W2767262730 @default.
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- W3198978829 cites W3092684442 @default.
- W3198978829 cites W3093120161 @default.
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- W3198978829 cites W3094352710 @default.
- W3198978829 cites W3096205573 @default.
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- W3198978829 cites W3102966306 @default.
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- W3198978829 cites W3112578401 @default.
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- W3198978829 doi "https://doi.org/10.3390/v13091814" @default.
- W3198978829 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8473059" @default.
- W3198978829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34578395" @default.
- W3198978829 hasPublicationYear "2021" @default.
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