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• W3198989457 abstract "Cultured cells are a very powerful tool for investigating biological events in vitro ; therefore, cell lines have been established not only in model insect species, but also in non-model species. However, there are few reports on the establishment of stable cell lines and development of systems to introduce genes into the cultured cells of the honey bee ( Apis mellifera ). We describe a simple ex vivo cell culture system for the honey bee. Hemocyte cells obtained from third and fourth instar larvae were cultured in commercial Grace’s insect medium or MGM-450 insect medium for more than two weeks maintaining a normal morphology without deterioration. After an expression plasmid vector bearing the enhanced green fluorescent protein ( egfp ) gene driven by the immediate early 2 (IE2) viral promoter was transfected into cells, EGFP fluorescence was detected in cells for more than one week from one day after transfection. Furthermore, double-stranded RNA corresponding to a part of the egfp gene was successfully introduced into cells and interfered with egfp gene expression. A convenient and reproducible method for an ex vivo cell culture that is fully practicable for gene expression assays was established for the honey bee." @default.
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• W3198989457 date "2021-09-23" @default.
• W3198989457 modified "2023-09-27" @default.
• W3198989457 title "A simple method for ex vivo honey bee cell culture capable of in vitro gene expression analysis" @default.
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• W3198989457 doi "https://doi.org/10.1371/journal.pone.0257770" @default.
• W3198989457 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8460014" @default.
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