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• W3199048638 abstract "Whole-genome sequence analysis of noroviruses is routinely performed by employing a metagenomic approach. While this methodology has several advantages, such as allowing for the examination of co-infection, it has some limitations, such as the requirement of high viral load to achieve full-length or near full-length genomic sequences. In this study, we used a pre-amplification step to obtain full-length genomic amplicons from 39 Canadian GII isolates, followed by deep sequencing on Illumina and Oxford Nanopore platforms. This approach significantly reduced the required viral titre to obtain full-genome coverage. Herein, we compared the coverage and sequences obtained by both platforms and provided an in-depth genomic analysis of the obtained sequences, including the presence of single-nucleotide variants and recombination events." @default.
• W3199048638 created "2021-09-27" @default.
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• W3199048638 date "2021-09-15" @default.
• W3199048638 modified "2023-10-16" @default.
• W3199048638 title "Genomic analysis of human noroviruses using combined Illumina–Nanopore data" @default.
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• W3199048638 doi "https://doi.org/10.1093/ve/veab079" @default.
• W3199048638 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35186325" @default.
• W3199048638 hasPublicationYear "2021" @default.
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