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• W3199156589 abstract "Holographic cytometry is introduced as an ultra-high throughput implementation of quantitative phase imaging of single cells flowing through parallel microfluidic channels. Here, the approach was applied for characterizing the morphology of individual red blood cells during storage under regular blood bank conditions. Samples from five blood donors were examined, over 100,000 cells examined for each, at three time points. The approach allows high-throughput phase imaging of a large number of cells, greatly extending our ability to study cellular phenotypes using individual cell images. Holographic cytology images can provide measurements of multiple physical traits of the cells, including optical volume and area, which are observed to consistently change over the storage time. In addition, the large volume of cell imaging data can serve as training data for machine-learning algorithms. For the study here, logistic regression was used to classify the cells according to the storage time points. The analysis showed that at least 5000 cells are needed to ensure accuracy of the classifiers. Overall, results showed the potential of holographic cytometry as a diagnostic tool." @default.
• W3199156589 created "2021-09-27" @default.
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• W3199156589 date "2021-09-17" @default.
• W3199156589 modified "2023-10-18" @default.
• W3199156589 title "Single Cell Analysis of Stored Red Blood Cells Using Ultra-High Throughput Holographic Cytometry" @default.
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• W3199156589 doi "https://doi.org/10.3390/cells10092455" @default.
• W3199156589 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8465484" @default.
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