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- W3199214249 abstract "We present a protocol for analyzing and evaluating the relocalization of proteins from the plasma membrane to chloroplasts. Some plant membrane-bound proteins carry dual targeting signals, e.g., a membrane-anchoring N-myristoylation motif and a chloroplast transit peptide. These proteins are predominantly targeted to membranes; upon certain cues, however, they can undergo detachment from membranes and relocalization to chloroplasts. This protocol combines imaging and biochemical analyses to track in a reliable and quantitative manner the relocalization of proteins between subcellular organelles. For complete details on the use and execution of this protocol, please refer to Medina-Puche et al. (2020)." @default.
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- W3199214249 date "2021-12-01" @default.
- W3199214249 modified "2023-10-02" @default.
- W3199214249 title "Protocol for evaluating protein relocalization from the plasma membrane to chloroplasts" @default.
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- W3199214249 doi "https://doi.org/10.1016/j.xpro.2021.100816" @default.
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