FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199326595> ?p ?o ?g. }

• W3199326595 endingPage "4662" @default.
• W3199326595 startingPage "4662" @default.
• W3199326595 abstract "Mismatch Repair (MMR) gene dysregulation plays a fundamental role in Lynch Syndrome (LS) pathogenesis, a form of hereditary colorectal cancer. Loss or overexpression of key MMR genes leads to genome instability and tumorigenesis; however, the mechanisms controlling MMR gene expression are unknown. One such gene, MSH2, exerts an important role, not only in MMR, but also in cell proliferation, apoptosis, and cell cycle control. In this study, we explored the functions and underlying molecular mechanisms of increased MSH2 expression related to a c.*226A>G variant in the 3′untranslated (UTR) region of MSH2 that had been previously identified in a subject clinically suspected of LS. Bioinformatics identified a putative binding site for miR-137 in this region. To verify miRNA targeting specificity, we performed luciferase gene reporter assays using a MSH2 3′UTR psiCHECK-2 vector in human SW480 cells over-expressing miR-137, which showed a drastic reduction in luciferase activity (p > 0.0001). This effect was abolished by site-directed mutagenesis of the putative miR-137 seed site. Moreover, in these cells we observed that miR-137 levels were inversely correlated with MSH2 expression levels. These results were confirmed by results in normal and tumoral tissues from the patient carrying the 3′UTR c.*226A>G variant in MSH2. Finally, miR-137 overexpression in SW480 cells significantly suppressed cell proliferation in a time- and dose-dependent manner (p < 0.0001), supporting a role for MSH2 in apoptosis and cell proliferation processes. Our findings suggest miR-137 helps control MSH2 expression via its 3′UTR and that dysregulation of this mechanism appears to promote tumorigenesis in colon cells." @default.
• W3199326595 created "2021-09-27" @default.
• W3199326595 creator A5013773492 @default.
• W3199326595 creator A5018120409 @default.
• W3199326595 creator A5041213852 @default.
• W3199326595 creator A5059105056 @default.
• W3199326595 creator A5061005482 @default.
• W3199326595 creator A5066944567 @default.
• W3199326595 creator A5072036478 @default.
• W3199326595 date "2021-09-17" @default.
• W3199326595 modified "2023-09-30" @default.
• W3199326595 title "MiR-137 Targets the 3′ Untranslated Region of MSH2: Potential Implications in Lynch Syndrome-Related Colorectal Cancer" @default.
• W3199326595 cites W1500930737 @default.
• W3199326595 cites W1575123465 @default.
• W3199326595 cites W1871677735 @default.
• W3199326595 cites W1946628128 @default.
• W3199326595 cites W1973803669 @default.
• W3199326595 cites W1975152594 @default.
• W3199326595 cites W1982824982 @default.
• W3199326595 cites W2025902983 @default.
• W3199326595 cites W2029102827 @default.
• W3199326595 cites W2033493684 @default.
• W3199326595 cites W2034455652 @default.
• W3199326595 cites W2072117239 @default.
• W3199326595 cites W2079200830 @default.
• W3199326595 cites W2085082345 @default.
• W3199326595 cites W2095671229 @default.
• W3199326595 cites W2099507518 @default.
• W3199326595 cites W2099952417 @default.
• W3199326595 cites W2111969156 @default.
• W3199326595 cites W2118715443 @default.
• W3199326595 cites W2119205528 @default.
• W3199326595 cites W2125523949 @default.
• W3199326595 cites W2126623769 @default.
• W3199326595 cites W2127970987 @default.
• W3199326595 cites W2162674813 @default.
• W3199326595 cites W2163106706 @default.
• W3199326595 cites W2163529853 @default.
• W3199326595 cites W2164749542 @default.
• W3199326595 cites W2222818901 @default.
• W3199326595 cites W2463603773 @default.
• W3199326595 cites W2465740235 @default.
• W3199326595 cites W2605845934 @default.
• W3199326595 cites W2771531415 @default.
• W3199326595 cites W2790852400 @default.
• W3199326595 cites W2792896330 @default.
• W3199326595 cites W2794445638 @default.
• W3199326595 cites W2801097407 @default.
• W3199326595 cites W2809932860 @default.
• W3199326595 cites W2888788301 @default.
• W3199326595 cites W2891024210 @default.
• W3199326595 cites W2910525258 @default.
• W3199326595 cites W2947550697 @default.
• W3199326595 cites W2955319991 @default.
• W3199326595 cites W2958079243 @default.
• W3199326595 cites W2970490879 @default.
• W3199326595 cites W2973269418 @default.
• W3199326595 cites W2994118818 @default.
• W3199326595 cites W3009345706 @default.
• W3199326595 cites W3013896039 @default.
• W3199326595 cites W3036710707 @default.
• W3199326595 cites W3037734947 @default.
• W3199326595 cites W3088916166 @default.
• W3199326595 cites W3134223534 @default.
• W3199326595 doi "https://doi.org/10.3390/cancers13184662" @default.
• W3199326595 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8470766" @default.
• W3199326595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34572889" @default.
• W3199326595 hasPublicationYear "2021" @default.
• W3199326595 type Work @default.
• W3199326595 sameAs 3199326595 @default.
• W3199326595 citedByCount "3" @default.
• W3199326595 countsByYear W31993265952022 @default.
• W3199326595 countsByYear W31993265952023 @default.
• W3199326595 crossrefType "journal-article" @default.
• W3199326595 hasAuthorship W3199326595A5013773492 @default.
• W3199326595 hasAuthorship W3199326595A5018120409 @default.
• W3199326595 hasAuthorship W3199326595A5041213852 @default.
• W3199326595 hasAuthorship W3199326595A5059105056 @default.
• W3199326595 hasAuthorship W3199326595A5061005482 @default.
• W3199326595 hasAuthorship W3199326595A5066944567 @default.
• W3199326595 hasAuthorship W3199326595A5072036478 @default.
• W3199326595 hasBestOaLocation W31993265951 @default.
• W3199326595 hasConcept C104317684 @default.
• W3199326595 hasConcept C105580179 @default.
• W3199326595 hasConcept C111335760 @default.
• W3199326595 hasConcept C121608353 @default.
• W3199326595 hasConcept C145059251 @default.
• W3199326595 hasConcept C153911025 @default.
• W3199326595 hasConcept C2776559941 @default.
• W3199326595 hasConcept C2779115348 @default.
• W3199326595 hasConcept C501734568 @default.
• W3199326595 hasConcept C502942594 @default.
• W3199326595 hasConcept C526805850 @default.
• W3199326595 hasConcept C54009773 @default.
• W3199326595 hasConcept C54355233 @default.
• W3199326595 hasConcept C555283112 @default.
• W3199326595 hasConcept C60748783 @default.
• W3199326595 hasConcept C62112901 @default.

• next