Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199340701> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W3199340701 abstract "Assessment of neural activity in the specific brain area is critical for understanding the circuit mechanisms underlying altered brain function and behaviors. A number of immediate early genes (IEGs) that are rapidly transcribed in neuronal cells in response to synaptic activity have been used as markers for neuronal activity. However, protein detection of IEGs requires translation, and the amount of newly synthesized gene product is usually insufficient to detect using western blotting, limiting their utility in western blot analysis of brain tissues for comparison of basal activity between control and genetically modified animals. Here, we show that the phosphorylation status of eukaryotic elongation factor-2 (eEF2) rapidly changes in response to synaptic and neural activities. Intraperitoneal injections of the GABA A receptor (GABAAR) antagonist picrotoxin and the glycine receptor antagonist brucine rapidly dephosphorylated eEF2. Conversely, potentiation of GABAARs or inhibition of AMPA receptors (AMPARs) induced rapid phosphorylation of eEF2 in both the hippocampus and forebrain of mice. Chemogenetic suppression of hippocampal principal neuron activity promoted eEF2 phosphorylation. Novel context exploration and acute restraint stress rapidly modified the phosphorylation status of hippocampal eEF2. Furthermore, the hippocampal eEF2 phosphorylation levels under basal conditions were reduced in mice exhibiting epilepsy and abnormally enhanced excitability in CA3 pyramidal neurons. Collectively, the results indicated that eEF2 phosphorylation status is sensitive to neural activity and the ratio of phosphorylated eEF2 to total eEF2 could be a molecular signature for estimating neural activity in a specific brain area." @default.
- W3199340701 created "2021-09-27" @default.
- W3199340701 creator A5017888782 @default.
- W3199340701 creator A5023718442 @default.
- W3199340701 creator A5054970112 @default.
- W3199340701 creator A5082436003 @default.
- W3199340701 creator A5087371439 @default.
- W3199340701 date "2021-09-15" @default.
- W3199340701 modified "2023-10-14" @default.
- W3199340701 title "The phosphorylation status of eukaryotic elongation factor-2 indicates neural activity in the brain" @default.
- W3199340701 cites W1583656960 @default.
- W3199340701 cites W1965917653 @default.
- W3199340701 cites W1969769253 @default.
- W3199340701 cites W2013319759 @default.
- W3199340701 cites W2043629406 @default.
- W3199340701 cites W2078108311 @default.
- W3199340701 cites W2078132872 @default.
- W3199340701 cites W2105663186 @default.
- W3199340701 cites W2112350473 @default.
- W3199340701 cites W2138969715 @default.
- W3199340701 cites W2746094764 @default.
- W3199340701 cites W3119418219 @default.
- W3199340701 cites W3119682802 @default.
- W3199340701 cites W3165606058 @default.
- W3199340701 doi "https://doi.org/10.1186/s13041-021-00852-0" @default.
- W3199340701 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8442277" @default.
- W3199340701 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34526091" @default.
- W3199340701 hasPublicationYear "2021" @default.
- W3199340701 type Work @default.
- W3199340701 sameAs 3199340701 @default.
- W3199340701 citedByCount "1" @default.
- W3199340701 countsByYear W31993407012022 @default.
- W3199340701 crossrefType "journal-article" @default.
- W3199340701 hasAuthorship W3199340701A5017888782 @default.
- W3199340701 hasAuthorship W3199340701A5023718442 @default.
- W3199340701 hasAuthorship W3199340701A5054970112 @default.
- W3199340701 hasAuthorship W3199340701A5082436003 @default.
- W3199340701 hasAuthorship W3199340701A5087371439 @default.
- W3199340701 hasBestOaLocation W31993407011 @default.
- W3199340701 hasConcept C104317684 @default.
- W3199340701 hasConcept C11960822 @default.
- W3199340701 hasConcept C148762608 @default.
- W3199340701 hasConcept C150194340 @default.
- W3199340701 hasConcept C169760540 @default.
- W3199340701 hasConcept C170493617 @default.
- W3199340701 hasConcept C25274449 @default.
- W3199340701 hasConcept C2777756961 @default.
- W3199340701 hasConcept C2779555244 @default.
- W3199340701 hasConcept C35599011 @default.
- W3199340701 hasConcept C515207424 @default.
- W3199340701 hasConcept C55493867 @default.
- W3199340701 hasConcept C86803240 @default.
- W3199340701 hasConcept C95444343 @default.
- W3199340701 hasConceptScore W3199340701C104317684 @default.
- W3199340701 hasConceptScore W3199340701C11960822 @default.
- W3199340701 hasConceptScore W3199340701C148762608 @default.
- W3199340701 hasConceptScore W3199340701C150194340 @default.
- W3199340701 hasConceptScore W3199340701C169760540 @default.
- W3199340701 hasConceptScore W3199340701C170493617 @default.
- W3199340701 hasConceptScore W3199340701C25274449 @default.
- W3199340701 hasConceptScore W3199340701C2777756961 @default.
- W3199340701 hasConceptScore W3199340701C2779555244 @default.
- W3199340701 hasConceptScore W3199340701C35599011 @default.
- W3199340701 hasConceptScore W3199340701C515207424 @default.
- W3199340701 hasConceptScore W3199340701C55493867 @default.
- W3199340701 hasConceptScore W3199340701C86803240 @default.
- W3199340701 hasConceptScore W3199340701C95444343 @default.
- W3199340701 hasFunder F4320322120 @default.
- W3199340701 hasIssue "1" @default.
- W3199340701 hasLocation W31993407011 @default.
- W3199340701 hasLocation W31993407012 @default.
- W3199340701 hasLocation W31993407013 @default.
- W3199340701 hasLocation W31993407014 @default.
- W3199340701 hasOpenAccess W3199340701 @default.
- W3199340701 hasPrimaryLocation W31993407011 @default.
- W3199340701 hasRelatedWork W1527061039 @default.
- W3199340701 hasRelatedWork W157755496 @default.
- W3199340701 hasRelatedWork W1968521183 @default.
- W3199340701 hasRelatedWork W2063583392 @default.
- W3199340701 hasRelatedWork W2069794312 @default.
- W3199340701 hasRelatedWork W2159426473 @default.
- W3199340701 hasRelatedWork W2441478541 @default.
- W3199340701 hasRelatedWork W2611240241 @default.
- W3199340701 hasRelatedWork W3134957322 @default.
- W3199340701 hasRelatedWork W4229646661 @default.
- W3199340701 hasVolume "14" @default.
- W3199340701 isParatext "false" @default.
- W3199340701 isRetracted "false" @default.
- W3199340701 magId "3199340701" @default.
- W3199340701 workType "article" @default.