Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199402404> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W3199402404 abstract "Hypothesis: Pigment epithelium derived factor (PEDF) interacts with low-density lipoprotein receptor-related protein 6 (Lrp6) to modulate wound angiogenesis. Objective: Previous studies demonstrate that anti-angiogenic proteins such as pigment epithelium-derived factor (PEDF) cause the pruning of capillaries in wounds and are critical to wound resolution. This study assesses the function of PEDF and its likely receptor, Lrp6, in wound angiogenesis. Methods: Adult PEDF -/- mice and wild-type littermate controls (C57 BL/6) (WT) were anesthetized, 3mm diameter skin wounds were prepared, and harvested at days 1, 3, 5, 7, 10, and 21 post-wounding. Vessel permeability was analyzed using fluorescein isothiocyanate (FITC) dextran. The effectiveness of siRNA transfection in reducing Lrp6 was demonstrated in vitro. Wounds were treated with Lrp6 siRNA or scrambled siRNA to examine the effect of Lrp6 knockdown on angiogenesis in vivo. Immunohistochemistry was performed to localize Lrp6 and to determine CD31+ blood vessel density in the wound bed. Results: Wound vascularity was significantly elevated in PEDF -/- compared to WT mice at days 0, 7, and 21. Vascular permeability, quantified using extra-vascular dextran, was also higher in PEDF -/- vs WT mice. Treatment of wounds with rPEDF caused an increase in Lrp6 expression on endothelial cells (EC) at day 10 (p=0.05). Lrp6 knockdown by siRNA caused a significant increase in wound angiogenesis in vivo (p<0.05). Conclusion: PEDF is required for proper vascular pruning and maturity in the resolving wound. PEDF stimulates the expression of Lrp6 on wound vasculature, a response that allows for appropriate endothelial responsiveness to PEDF. Inhibition of Lrp6 leads to increased wound vascularity, suggesting that PEDF acts through this receptor. The findings indicate that the interaction of PEDF with LRP6 is critical for the regulation of wound angiogenesis." @default.
- W3199402404 created "2021-09-27" @default.
- W3199402404 creator A5008918435 @default.
- W3199402404 date "2018-01-01" @default.
- W3199402404 modified "2023-09-26" @default.
- W3199402404 title "Regulation of Angiogenesis in Healing Wounds" @default.
- W3199402404 hasPublicationYear "2018" @default.
- W3199402404 type Work @default.
- W3199402404 sameAs 3199402404 @default.
- W3199402404 citedByCount "0" @default.
- W3199402404 crossrefType "journal-article" @default.
- W3199402404 hasAuthorship W3199402404A5008918435 @default.
- W3199402404 hasConcept C150903083 @default.
- W3199402404 hasConcept C173396325 @default.
- W3199402404 hasConcept C190283241 @default.
- W3199402404 hasConcept C203014093 @default.
- W3199402404 hasConcept C207001950 @default.
- W3199402404 hasConcept C2778271429 @default.
- W3199402404 hasConcept C2778939058 @default.
- W3199402404 hasConcept C2780269544 @default.
- W3199402404 hasConcept C2780394083 @default.
- W3199402404 hasConcept C502942594 @default.
- W3199402404 hasConcept C52237779 @default.
- W3199402404 hasConcept C55493867 @default.
- W3199402404 hasConcept C71924100 @default.
- W3199402404 hasConcept C86803240 @default.
- W3199402404 hasConceptScore W3199402404C150903083 @default.
- W3199402404 hasConceptScore W3199402404C173396325 @default.
- W3199402404 hasConceptScore W3199402404C190283241 @default.
- W3199402404 hasConceptScore W3199402404C203014093 @default.
- W3199402404 hasConceptScore W3199402404C207001950 @default.
- W3199402404 hasConceptScore W3199402404C2778271429 @default.
- W3199402404 hasConceptScore W3199402404C2778939058 @default.
- W3199402404 hasConceptScore W3199402404C2780269544 @default.
- W3199402404 hasConceptScore W3199402404C2780394083 @default.
- W3199402404 hasConceptScore W3199402404C502942594 @default.
- W3199402404 hasConceptScore W3199402404C52237779 @default.
- W3199402404 hasConceptScore W3199402404C55493867 @default.
- W3199402404 hasConceptScore W3199402404C71924100 @default.
- W3199402404 hasConceptScore W3199402404C86803240 @default.
- W3199402404 hasLocation W31994024041 @default.
- W3199402404 hasOpenAccess W3199402404 @default.
- W3199402404 hasPrimaryLocation W31994024041 @default.
- W3199402404 hasRelatedWork W1967337383 @default.
- W3199402404 hasRelatedWork W2014154081 @default.
- W3199402404 hasRelatedWork W2016933731 @default.
- W3199402404 hasRelatedWork W2025693553 @default.
- W3199402404 hasRelatedWork W2090721821 @default.
- W3199402404 hasRelatedWork W2155954517 @default.
- W3199402404 hasRelatedWork W2158302648 @default.
- W3199402404 hasRelatedWork W2167037305 @default.
- W3199402404 hasRelatedWork W2281938063 @default.
- W3199402404 hasRelatedWork W2356754941 @default.
- W3199402404 hasRelatedWork W2400138938 @default.
- W3199402404 hasRelatedWork W2409345107 @default.
- W3199402404 hasRelatedWork W2530504519 @default.
- W3199402404 hasRelatedWork W2765284459 @default.
- W3199402404 hasRelatedWork W2885608399 @default.
- W3199402404 hasRelatedWork W2950969405 @default.
- W3199402404 hasRelatedWork W2999846173 @default.
- W3199402404 hasRelatedWork W3011613764 @default.
- W3199402404 hasRelatedWork W3189788754 @default.
- W3199402404 hasRelatedWork W818886044 @default.
- W3199402404 isParatext "false" @default.
- W3199402404 isRetracted "false" @default.
- W3199402404 magId "3199402404" @default.
- W3199402404 workType "article" @default.