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- W3199495500 abstract "In this work, a DNA three-way junction (TWJ) with multiple recognition regions was intelligently engineered, which could be applied as an unconfined DNA walker with a rapid walking speed and high sensitivity for electrochemical detection of microRNA (miRNA-182-5p). Once the target miRNA was presented, the hairpins on TWJ could be successively opened to form an annular DNA walker, which could walk on the entire scope of the electrode surface without the confine for the length of DNA walker legs compared with the traditional DNA walker, greatly improving the walking efficiency. In addition, this DNA walker with multirecognition segments could obviously increase the local concentration of recognition sites, which significantly enhanced the detection speed and sensitivity. As a result, this proposed biosensor with annular DNA as a walker could dexterously achieve the ultrasensitive and fast detection of miRNA-182-5p from 0.1 fM to 1 nM with a detection limit of 31.13 aM. Meaningfully, this strategy explored an innovative path in the design of a new DNA walker nanostructure for accomplishing speedy and sensitive detection of biomarkers." @default.
- W3199495500 created "2021-09-27" @default.
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- W3199495500 date "2021-09-13" @default.
- W3199495500 modified "2023-10-18" @default.
- W3199495500 title "DNA Three-Way Junction with Multiple Recognition Regions Mediated an Unconfined DNA Walker for Electrochemical Ultrasensitive Detection of miRNA-182-5p" @default.
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- W3199495500 doi "https://doi.org/10.1021/acs.analchem.1c02668" @default.
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