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• W3199535105 abstract "ABSTRACT The storage of data in DNA typically involves encoding and synthesizing data into short oligonucleotides, followed by reading with a sequencing instrument. Major challenges include the molecular consumption of synthesized DNA, issues with basecalling errors, and limitations with scaling up read access operations for individual data elements. Addressing these challenges, we describe a DNA storage system called MDRAM ( M agnetic D NA-based R andom A ccess M emory) that enables repetitive and efficient readouts of targeted files with nanopore-based sequencing. Through conjugation of synthesized DNA to magnetic beads, we enabled repeated readouts of data while preserving the original DNA analyte and maintaining data readout quality. MDRAM also utilizes an efficient convolutional coding scheme that leverages soft information in raw nanopore sequencing signals to achieve information reading costs comparable to Illumina sequencing despite substantially higher error rates. Finally, we demonstrate a proof-of-concept DNA-based proto-filesystem that enables an exponentially-scalable data address space using only small numbers of targeting primers for assembly and readout. ONE-SENTENCE SUMMARY We demonstrate a novel DNA data storage system that leverages conjugation of DNA onto magnetic beads, new computational advances in data encoding, and exponentially scalable access of individual data elements." @default.
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• W3199535105 date "2021-09-16" @default.
• W3199535105 modified "2023-10-17" @default.
• W3199535105 title "Magnetic DNA random access memory with nanopore readouts and exponentially-scaled combinatorial addressing" @default.
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• W3199535105 doi "https://doi.org/10.1101/2021.09.15.460571" @default.
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