Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199573521> ?p ?o ?g. }
- W3199573521 abstract "Labeling of biomolecules with a paramagnetic probe for nuclear magnetic resonance (NMR) spectroscopy enables determining long-range distance restraints, which are otherwise not accessible by classically used dipolar coupling-based NMR approaches. Distance restraints derived from paramagnetic relaxation enhancements (PREs) can facilitate the structure determination of large proteins and protein complexes. We herein present the site-directed labeling of the large oligomeric bacterial DnaB helicase from Helicobacter pylori with cysteine-reactive maleimide tags carrying either a nitroxide radical or a lanthanide ion. The success of the labeling reaction was followed by quantitative continuous-wave electron paramagnetic resonance (EPR) experiments performed on the nitroxide-labeled protein. PREs were extracted site-specifically from 2D and 3D solid-state NMR spectra. A good agreement with predicted PRE values, derived by computational modeling of nitroxide and Gd3+ tags in the low-resolution DnaB crystal structure, was found. Comparison of experimental PREs and model-predicted spin label-nucleus distances indicated that the size of the blind sphere around the paramagnetic center, in which NMR resonances are not detected, is slightly larger for Gd3+ (~14 Å) than for nitroxide (~11 Å) in 13C-detected 2D spectra of DnaB. We also present Gd3+-Gd3+ dipolar electron-electron resonance EPR experiments on DnaB supporting the conclusion that DnaB was present as a hexameric assembly." @default.
- W3199573521 created "2021-09-27" @default.
- W3199573521 creator A5028513157 @default.
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- W3199573521 date "2021-09-14" @default.
- W3199573521 modified "2023-10-16" @default.
- W3199573521 title "Paramagnetic spin labeling of a bacterial DnaB helicase for solid-state NMR" @default.
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- W3199573521 doi "https://doi.org/10.1101/2021.09.14.460235" @default.
- W3199573521 hasPublicationYear "2021" @default.
- W3199573521 type Work @default.