Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199677768> ?p ?o ?g. }
- W3199677768 abstract "Significance: The recently reported solvent-based optical clearing method FDISCO can preserve various fluorescent signals very well. However, the strict low-temperature storage condition of FDISCO is not conducive to long-time or repetitive imaging usually conducted at room temperature. Therefore, it is important to solve the contradiction between fluorescence preservation and imaging condition. Aim: We develop a modified FDISCO clearing method, termed FDISCO+, to change the preservation condition from low temperature to room temperature. Approach: Two alternative antioxidants were screened out to effectively inhibit the peroxide generation in the clearing agent at room temperature, enabling robust fluorescence preservation of cleared samples. Results: FDISCO+ achieves comparable fluorescence preservation with the original FDISCO protocol and allows long-time storage at room temperature, making it easier for researchers to image and preserve the samples. Conclusions: FDISCO+ is expected to be widely used due to its loose operation requirements." @default.
- W3199677768 created "2021-09-27" @default.
- W3199677768 creator A5014038359 @default.
- W3199677768 creator A5034322811 @default.
- W3199677768 creator A5045467934 @default.
- W3199677768 creator A5051129335 @default.
- W3199677768 creator A5055341024 @default.
- W3199677768 creator A5077930196 @default.
- W3199677768 creator A5090408728 @default.
- W3199677768 date "2021-09-09" @default.
- W3199677768 modified "2023-10-16" @default.
- W3199677768 title "FDISCO+: a clearing method for robust fluorescence preservation of cleared samples" @default.
- W3199677768 cites W1570094373 @default.
- W3199677768 cites W1570588423 @default.
- W3199677768 cites W1603300429 @default.
- W3199677768 cites W1964049834 @default.
- W3199677768 cites W1975389666 @default.
- W3199677768 cites W1978590690 @default.
- W3199677768 cites W2010114799 @default.
- W3199677768 cites W2010956007 @default.
- W3199677768 cites W2031025318 @default.
- W3199677768 cites W2037884629 @default.
- W3199677768 cites W2047242615 @default.
- W3199677768 cites W2049555570 @default.
- W3199677768 cites W2051438014 @default.
- W3199677768 cites W2052397345 @default.
- W3199677768 cites W2068391384 @default.
- W3199677768 cites W2076507262 @default.
- W3199677768 cites W2078677652 @default.
- W3199677768 cites W2083048982 @default.
- W3199677768 cites W2087451538 @default.
- W3199677768 cites W2094314563 @default.
- W3199677768 cites W2121011070 @default.
- W3199677768 cites W2203529929 @default.
- W3199677768 cites W2208442878 @default.
- W3199677768 cites W2296648384 @default.
- W3199677768 cites W2296740725 @default.
- W3199677768 cites W2499712993 @default.
- W3199677768 cites W2512571444 @default.
- W3199677768 cites W2583133224 @default.
- W3199677768 cites W2746997939 @default.
- W3199677768 cites W2762921063 @default.
- W3199677768 cites W2785680713 @default.
- W3199677768 cites W2806488757 @default.
- W3199677768 cites W2887634581 @default.
- W3199677768 cites W2888024351 @default.
- W3199677768 cites W2906442564 @default.
- W3199677768 cites W2910039853 @default.
- W3199677768 cites W2911943035 @default.
- W3199677768 cites W2932099806 @default.
- W3199677768 cites W2974311809 @default.
- W3199677768 cites W2993468371 @default.
- W3199677768 cites W3008250542 @default.
- W3199677768 cites W3120942201 @default.
- W3199677768 cites W3129996795 @default.
- W3199677768 doi "https://doi.org/10.1117/1.nph.8.3.035007" @default.
- W3199677768 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8427119" @default.
- W3199677768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34514032" @default.
- W3199677768 hasPublicationYear "2021" @default.
- W3199677768 type Work @default.
- W3199677768 sameAs 3199677768 @default.
- W3199677768 citedByCount "3" @default.
- W3199677768 countsByYear W31996777682022 @default.
- W3199677768 countsByYear W31996777682023 @default.
- W3199677768 crossrefType "journal-article" @default.
- W3199677768 hasAuthorship W3199677768A5014038359 @default.
- W3199677768 hasAuthorship W3199677768A5034322811 @default.
- W3199677768 hasAuthorship W3199677768A5045467934 @default.
- W3199677768 hasAuthorship W3199677768A5051129335 @default.
- W3199677768 hasAuthorship W3199677768A5055341024 @default.
- W3199677768 hasAuthorship W3199677768A5077930196 @default.
- W3199677768 hasAuthorship W3199677768A5090408728 @default.
- W3199677768 hasBestOaLocation W31996777681 @default.
- W3199677768 hasConcept C10138342 @default.
- W3199677768 hasConcept C120665830 @default.
- W3199677768 hasConcept C121332964 @default.
- W3199677768 hasConcept C126894567 @default.
- W3199677768 hasConcept C127413603 @default.
- W3199677768 hasConcept C134697681 @default.
- W3199677768 hasConcept C138944611 @default.
- W3199677768 hasConcept C162324750 @default.
- W3199677768 hasConcept C179687394 @default.
- W3199677768 hasConcept C186060115 @default.
- W3199677768 hasConcept C21880701 @default.
- W3199677768 hasConcept C41008148 @default.
- W3199677768 hasConcept C71924100 @default.
- W3199677768 hasConcept C86803240 @default.
- W3199677768 hasConcept C91881484 @default.
- W3199677768 hasConceptScore W3199677768C10138342 @default.
- W3199677768 hasConceptScore W3199677768C120665830 @default.
- W3199677768 hasConceptScore W3199677768C121332964 @default.
- W3199677768 hasConceptScore W3199677768C126894567 @default.
- W3199677768 hasConceptScore W3199677768C127413603 @default.
- W3199677768 hasConceptScore W3199677768C134697681 @default.
- W3199677768 hasConceptScore W3199677768C138944611 @default.
- W3199677768 hasConceptScore W3199677768C162324750 @default.
- W3199677768 hasConceptScore W3199677768C179687394 @default.
- W3199677768 hasConceptScore W3199677768C186060115 @default.
- W3199677768 hasConceptScore W3199677768C21880701 @default.
- W3199677768 hasConceptScore W3199677768C41008148 @default.