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- W3199764132 abstract "Little is known about the diversity and function of adipose tissue nerves, due in part to the inability to effectively visualize the tissue’s diverse nerve subtypes and the patterns of innervation across an intact depot. The tools to image and quantify adipose tissue innervation are currently limited. Here, we present a method of tissue processing that decreases tissue thickness in the z-axis while leaving cells intact for subsequent immunostaining. This was combined with autofluorescence quenching techniques to permit intact whole tissues to be mounted on slides and imaged by confocal microscopy, with a complementary means to perform whole tissue neurite density quantification after capture of tiled z-stack images. Additionally, we demonstrate how to visualize nerve terminals (the neuro-adipose nexus) in intact blocks of adipose tissue without z-depth reduction. We have included examples of data demonstrating nerve subtypes, neurovascular interactions, label-free imaging of collagen, and nerve bundle digital cross-sections." @default.
- W3199764132 created "2021-09-27" @default.
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- W3199764132 date "2021-10-01" @default.
- W3199764132 modified "2023-10-18" @default.
- W3199764132 title "Visualization and analysis of whole depot adipose tissue neural innervation" @default.
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- W3199764132 doi "https://doi.org/10.1016/j.isci.2021.103127" @default.
- W3199764132 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8479257" @default.
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