FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199799483> ?p ?o ?g. }

• W3199799483 endingPage "2477" @default.
• W3199799483 startingPage "2465" @default.
• W3199799483 abstract "Minicells, small cells lacking a chromosome, produced by bacteria with mutated min genes, which control cell division septum placement, have many potential uses. Minicells have contributed to basic bacterial physiology studies and can enable new biotechnological applications, including drug delivery and vaccines. Genome-reduced bacteria are another informative area of investigation. Investigators identified that with even almost 30% of the E. coli genome deleted, the bacteria still live. In biotechnology and synthetic biology, genome-reduced bacteria offer certain advantages. With genome-reduced bacteria, more recombinant genes can be placed into genome-reduced chromosomes and fewer cell resources are devoted to purposes apart from biotechnological goals. Here, we show that these two technologies can be combined: min mutants can be made in genome-reduced E. coli. The minC minD mutant genome-reduced E. coli produce minicells that concentrate engineered recombinant proteins within these spherical delivery systems. We expressed recombinant GFP protein in the cytoplasm of genome-reduced bacteria and showed that it is concentrated within the minicells. We also expressed proteins on the surfaces of minicells made from genome-reduced bacteria using a recombinant Gram-negative AIDA-I autotransporter expression cassette. Some autotransporters, like AIDA-I, are concentrated at the bacterial poles, where minicells bud. Recombinant proteins expressed on surfaces of the genome-reduced bacteria are concentrated on the minicells. Minicells made from genome-reduced bacteria may enable useful biotechnological innovations, such as drug delivery vehicles and vaccine immunogens." @default.
• W3199799483 created "2021-09-27" @default.
• W3199799483 creator A5005520907 @default.
• W3199799483 creator A5006339782 @default.
• W3199799483 creator A5040027394 @default.
• W3199799483 creator A5045455811 @default.
• W3199799483 creator A5053037091 @default.
• W3199799483 creator A5059536308 @default.
• W3199799483 creator A5059706312 @default.
• W3199799483 creator A5062808829 @default.
• W3199799483 creator A5070844318 @default.
• W3199799483 creator A5082120737 @default.
• W3199799483 date "2021-09-13" @default.
• W3199799483 modified "2023-09-26" @default.
• W3199799483 title "Minicells from Highly Genome Reduced <i>Escherichia coli</i>: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells" @default.
• W3199799483 cites W1588869115 @default.
• W3199799483 cites W1849866368 @default.
• W3199799483 cites W1960529837 @default.
• W3199799483 cites W1973728570 @default.
• W3199799483 cites W1980185753 @default.
• W3199799483 cites W1986796454 @default.
• W3199799483 cites W1991738972 @default.
• W3199799483 cites W1995485942 @default.
• W3199799483 cites W2004466255 @default.
• W3199799483 cites W2006721213 @default.
• W3199799483 cites W2018532552 @default.
• W3199799483 cites W2033350939 @default.
• W3199799483 cites W2036254354 @default.
• W3199799483 cites W2047998262 @default.
• W3199799483 cites W2080560063 @default.
• W3199799483 cites W2092571964 @default.
• W3199799483 cites W2093597024 @default.
• W3199799483 cites W2097381450 @default.
• W3199799483 cites W2108385415 @default.
• W3199799483 cites W2116137883 @default.
• W3199799483 cites W2120785022 @default.
• W3199799483 cites W2140901540 @default.
• W3199799483 cites W2141150007 @default.
• W3199799483 cites W2159786000 @default.
• W3199799483 cites W2167210281 @default.
• W3199799483 cites W2256496133 @default.
• W3199799483 cites W2558107185 @default.
• W3199799483 cites W2566894387 @default.
• W3199799483 cites W2885581383 @default.
• W3199799483 cites W2897540414 @default.
• W3199799483 cites W2904314998 @default.
• W3199799483 cites W2947125761 @default.
• W3199799483 cites W3036249600 @default.
• W3199799483 cites W3135922908 @default.
• W3199799483 cites W3153169670 @default.
• W3199799483 cites W2314868493 @default.
• W3199799483 doi "https://doi.org/10.1021/acssynbio.1c00375" @default.
• W3199799483 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34516078" @default.
• W3199799483 hasPublicationYear "2021" @default.
• W3199799483 type Work @default.
• W3199799483 sameAs 3199799483 @default.
• W3199799483 citedByCount "1" @default.
• W3199799483 countsByYear W31997994832023 @default.
• W3199799483 crossrefType "journal-article" @default.
• W3199799483 hasAuthorship W3199799483A5005520907 @default.
• W3199799483 hasAuthorship W3199799483A5006339782 @default.
• W3199799483 hasAuthorship W3199799483A5040027394 @default.
• W3199799483 hasAuthorship W3199799483A5045455811 @default.
• W3199799483 hasAuthorship W3199799483A5053037091 @default.
• W3199799483 hasAuthorship W3199799483A5059536308 @default.
• W3199799483 hasAuthorship W3199799483A5059706312 @default.
• W3199799483 hasAuthorship W3199799483A5062808829 @default.
• W3199799483 hasAuthorship W3199799483A5070844318 @default.
• W3199799483 hasAuthorship W3199799483A5082120737 @default.
• W3199799483 hasBestOaLocation W31997994832 @default.
• W3199799483 hasConcept C104317684 @default.
• W3199799483 hasConcept C141231307 @default.
• W3199799483 hasConcept C190062978 @default.
• W3199799483 hasConcept C3742359 @default.
• W3199799483 hasConcept C40767141 @default.
• W3199799483 hasConcept C523546767 @default.
• W3199799483 hasConcept C54355233 @default.
• W3199799483 hasConcept C547475151 @default.
• W3199799483 hasConcept C58167604 @default.
• W3199799483 hasConcept C86803240 @default.
• W3199799483 hasConcept C89423630 @default.
• W3199799483 hasConceptScore W3199799483C104317684 @default.
• W3199799483 hasConceptScore W3199799483C141231307 @default.
• W3199799483 hasConceptScore W3199799483C190062978 @default.
• W3199799483 hasConceptScore W3199799483C3742359 @default.
• W3199799483 hasConceptScore W3199799483C40767141 @default.
• W3199799483 hasConceptScore W3199799483C523546767 @default.
• W3199799483 hasConceptScore W3199799483C54355233 @default.
• W3199799483 hasConceptScore W3199799483C547475151 @default.
• W3199799483 hasConceptScore W3199799483C58167604 @default.
• W3199799483 hasConceptScore W3199799483C86803240 @default.
• W3199799483 hasConceptScore W3199799483C89423630 @default.
• W3199799483 hasFunder F4320306300 @default.
• W3199799483 hasFunder F4320310536 @default.
• W3199799483 hasIssue "10" @default.
• W3199799483 hasLocation W31997994831 @default.
• W3199799483 hasLocation W31997994832 @default.
• W3199799483 hasLocation W31997994833 @default.

• next