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- W3199868450 endingPage "100130" @default.
- W3199868450 startingPage "100130" @default.
- W3199868450 abstract "During in vitro culture, bereft of their optimal tissue context, tenocytes lose their phenotype and function. Considering that tenocytes in their native tissue milieu are exposed simultaneously to manifold signals, combination approaches (e.g. growth factor supplementation and mechanical stimulation) are continuously gaining pace to control cell fate during in vitro expansion, albeit with limited success due to the literally infinite number of possible permutations. In this work, we assessed the potential of scalable and potent physicochemical approaches that control cell fate (substrate stiffness, anisotropic surface topography, collagen type I coating) and enhance extracellular matrix deposition (macromolecular crowding) in maintaining human tenocyte phenotype in culture. Cell morphology was primarily responsive to surface topography. The tissue culture plastic induced the largest nuclei area, the lowest aspect ratio, and the highest focal adhesion kinase. Collagen type I coating increased cell number and metabolic activity. Cell viability was not affected by any of the variables assessed. Macromolecular crowding intensely enhanced and accelerated native extracellular matrix deposition, albeit not in an aligned fashion, even on the grooved substrates. Gene analysis at day 14 revealed that the 130 kPa grooved substrate without collagen type I coating and under macromolecular crowding conditions positively regulated human tenocyte phenotype. Collectively, this work illustrates the beneficial effects of combined physicochemical approaches in controlling cell fate during in vitro expansion." @default.
- W3199868450 created "2021-09-27" @default.
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- W3199868450 date "2021-09-01" @default.
- W3199868450 modified "2023-10-14" @default.
- W3199868450 title "A combined physicochemical approach towards human tenocyte phenotype maintenance" @default.
- W3199868450 cites W1487169871 @default.
- W3199868450 cites W1578480468 @default.
- W3199868450 cites W1812743645 @default.
- W3199868450 cites W1820602848 @default.
- W3199868450 cites W1912441301 @default.
- W3199868450 cites W1921978279 @default.
- W3199868450 cites W1962348298 @default.
- W3199868450 cites W1963892111 @default.
- W3199868450 cites W1964042376 @default.
- W3199868450 cites W1966517984 @default.
- W3199868450 cites W1970294584 @default.
- W3199868450 cites W1974935282 @default.
- W3199868450 cites W1977989441 @default.
- W3199868450 cites W1978288081 @default.
- W3199868450 cites W1980474802 @default.
- W3199868450 cites W1980628034 @default.
- W3199868450 cites W1982876681 @default.
- W3199868450 cites W1983268178 @default.
- W3199868450 cites W1983451792 @default.
- W3199868450 cites W1984900053 @default.
- W3199868450 cites W1989275521 @default.
- W3199868450 cites W1990748729 @default.
- W3199868450 cites W1993350514 @default.
- W3199868450 cites W1993483760 @default.
- W3199868450 cites W1994112085 @default.
- W3199868450 cites W1994408107 @default.
- W3199868450 cites W1994423139 @default.
- W3199868450 cites W1995007281 @default.
- W3199868450 cites W1996773341 @default.
- W3199868450 cites W1998925963 @default.
- W3199868450 cites W2000156083 @default.
- W3199868450 cites W2000163722 @default.
- W3199868450 cites W2004734519 @default.
- W3199868450 cites W2009823096 @default.
- W3199868450 cites W2009981178 @default.
- W3199868450 cites W2010368477 @default.
- W3199868450 cites W2011628476 @default.
- W3199868450 cites W2013130843 @default.
- W3199868450 cites W2014098416 @default.
- W3199868450 cites W2015642650 @default.
- W3199868450 cites W2015979204 @default.
- W3199868450 cites W2018882303 @default.
- W3199868450 cites W2024250498 @default.
- W3199868450 cites W2024813579 @default.
- W3199868450 cites W2024921332 @default.
- W3199868450 cites W2026316716 @default.
- W3199868450 cites W2029551351 @default.
- W3199868450 cites W2030568617 @default.
- W3199868450 cites W2030792058 @default.
- W3199868450 cites W2035760910 @default.
- W3199868450 cites W2037083085 @default.
- W3199868450 cites W2037488682 @default.
- W3199868450 cites W2038097126 @default.
- W3199868450 cites W2038722569 @default.
- W3199868450 cites W2039960669 @default.
- W3199868450 cites W2040258779 @default.
- W3199868450 cites W2041973808 @default.
- W3199868450 cites W2042258234 @default.
- W3199868450 cites W2042671584 @default.
- W3199868450 cites W2042840274 @default.
- W3199868450 cites W2043634767 @default.
- W3199868450 cites W2044977073 @default.
- W3199868450 cites W2045558559 @default.
- W3199868450 cites W2048104669 @default.
- W3199868450 cites W2050234611 @default.
- W3199868450 cites W2050896806 @default.
- W3199868450 cites W2053117318 @default.
- W3199868450 cites W2054500578 @default.
- W3199868450 cites W2055978912 @default.
- W3199868450 cites W2056206562 @default.
- W3199868450 cites W2062177658 @default.
- W3199868450 cites W2062628989 @default.
- W3199868450 cites W2064481190 @default.
- W3199868450 cites W2067011858 @default.
- W3199868450 cites W2067114363 @default.
- W3199868450 cites W2067716147 @default.
- W3199868450 cites W2067933702 @default.
- W3199868450 cites W2070235232 @default.
- W3199868450 cites W2072293064 @default.
- W3199868450 cites W2074996103 @default.
- W3199868450 cites W2075130086 @default.
- W3199868450 cites W2077024797 @default.
- W3199868450 cites W2077633415 @default.