Matches in SemOpenAlex for { <https://semopenalex.org/work/W3199892757> ?p ?o ?g. }
- W3199892757 endingPage "10012" @default.
- W3199892757 startingPage "10012" @default.
- W3199892757 abstract "The STEAP1 is a cell-surface antigen over-expressed in prostate cancer, which contributes to tumor progression and aggressiveness. However, the molecular mechanisms underlying STEAP1 and its structural determinants remain elusive.The fraction capacity of Butyl- and Octyl-Sepharose matrices on LNCaP lysates was evaluated by manipulating the ionic strength of binding and elution phases, followed by a Co-Immunoprecipitation (Co-IP) polishing. Several potential stabilizing additives were assessed, and the melting temperature (Tm) values ranked the best/worst compounds. The secondary structure of STEAP1 was identified by circular dichroism.The STEAP1 was not fully captured with 1.375 M (Butyl), in contrast with interfering heterologous proteins, which were strongly retained and mostly eluted with water. This single step demonstrated higher selectivity of Butyl-Sepharose for host impurities removal from injected crude samples. Co-IP allowed recovering a purified fraction of STEAP1 and contributed to unveil potential physiologically interacting counterparts with the target. A Tm of ~55 °C was determined, confirming STEAP1 stability in the purification buffer. A predominant α-helical structure was identified, ensuring the protein's structural stability.A method for successfully isolating human STEAP1 from LNCaP cells was provided, avoiding the use of detergents to achieve stability, even outside a membrane-mimicking environment." @default.
- W3199892757 created "2021-09-27" @default.
- W3199892757 creator A5025140163 @default.
- W3199892757 creator A5040461350 @default.
- W3199892757 creator A5049238909 @default.
- W3199892757 creator A5049252324 @default.
- W3199892757 creator A5060607126 @default.
- W3199892757 creator A5073620885 @default.
- W3199892757 creator A5074740547 @default.
- W3199892757 date "2021-09-16" @default.
- W3199892757 modified "2023-09-26" @default.
- W3199892757 title "Enhanced Stability of Detergent-Free Human Native STEAP1 Protein from Neoplastic Prostate Cancer Cells upon an Innovative Isolation Procedure" @default.
- W3199892757 cites W1128661185 @default.
- W3199892757 cites W1487052430 @default.
- W3199892757 cites W1835920717 @default.
- W3199892757 cites W1970027357 @default.
- W3199892757 cites W1975610085 @default.
- W3199892757 cites W1977353424 @default.
- W3199892757 cites W2001904774 @default.
- W3199892757 cites W2002381200 @default.
- W3199892757 cites W2006753200 @default.
- W3199892757 cites W2008240057 @default.
- W3199892757 cites W2011296430 @default.
- W3199892757 cites W2021572649 @default.
- W3199892757 cites W2046487933 @default.
- W3199892757 cites W2047467092 @default.
- W3199892757 cites W2055879427 @default.
- W3199892757 cites W2066561369 @default.
- W3199892757 cites W2072192609 @default.
- W3199892757 cites W2073615736 @default.
- W3199892757 cites W2082145097 @default.
- W3199892757 cites W2100837269 @default.
- W3199892757 cites W2107959310 @default.
- W3199892757 cites W2125108879 @default.
- W3199892757 cites W2137599433 @default.
- W3199892757 cites W2138865250 @default.
- W3199892757 cites W2140398200 @default.
- W3199892757 cites W2143550120 @default.
- W3199892757 cites W2147138865 @default.
- W3199892757 cites W2161994209 @default.
- W3199892757 cites W2169547796 @default.
- W3199892757 cites W2190424885 @default.
- W3199892757 cites W2274268721 @default.
- W3199892757 cites W2288830688 @default.
- W3199892757 cites W2460414839 @default.
- W3199892757 cites W2539417429 @default.
- W3199892757 cites W2542964897 @default.
- W3199892757 cites W2610868722 @default.
- W3199892757 cites W2614728412 @default.
- W3199892757 cites W2788740682 @default.
- W3199892757 cites W2896690111 @default.
- W3199892757 cites W2909702116 @default.
- W3199892757 cites W2911775629 @default.
- W3199892757 cites W2918630809 @default.
- W3199892757 cites W2942545849 @default.
- W3199892757 cites W2969560376 @default.
- W3199892757 cites W2971945336 @default.
- W3199892757 cites W2972516291 @default.
- W3199892757 cites W2982879405 @default.
- W3199892757 cites W3024314955 @default.
- W3199892757 cites W3037653991 @default.
- W3199892757 cites W3094582821 @default.
- W3199892757 cites W3165328322 @default.
- W3199892757 doi "https://doi.org/10.3390/ijms221810012" @default.
- W3199892757 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8472055" @default.
- W3199892757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34576175" @default.
- W3199892757 hasPublicationYear "2021" @default.
- W3199892757 type Work @default.
- W3199892757 sameAs 3199892757 @default.
- W3199892757 citedByCount "5" @default.
- W3199892757 countsByYear W31998927572022 @default.
- W3199892757 countsByYear W31998927572023 @default.
- W3199892757 crossrefType "journal-article" @default.
- W3199892757 hasAuthorship W3199892757A5025140163 @default.
- W3199892757 hasAuthorship W3199892757A5040461350 @default.
- W3199892757 hasAuthorship W3199892757A5049238909 @default.
- W3199892757 hasAuthorship W3199892757A5049252324 @default.
- W3199892757 hasAuthorship W3199892757A5060607126 @default.
- W3199892757 hasAuthorship W3199892757A5073620885 @default.
- W3199892757 hasAuthorship W3199892757A5074740547 @default.
- W3199892757 hasBestOaLocation W31998927571 @default.
- W3199892757 hasConcept C121608353 @default.
- W3199892757 hasConcept C12554922 @default.
- W3199892757 hasConcept C133571119 @default.
- W3199892757 hasConcept C178790620 @default.
- W3199892757 hasConcept C184651966 @default.
- W3199892757 hasConcept C185592680 @default.
- W3199892757 hasConcept C187428577 @default.
- W3199892757 hasConcept C2779723316 @default.
- W3199892757 hasConcept C2780192828 @default.
- W3199892757 hasConcept C43617362 @default.
- W3199892757 hasConcept C54355233 @default.
- W3199892757 hasConcept C55493867 @default.
- W3199892757 hasConcept C73826308 @default.
- W3199892757 hasConcept C86803240 @default.
- W3199892757 hasConceptScore W3199892757C121608353 @default.
- W3199892757 hasConceptScore W3199892757C12554922 @default.
- W3199892757 hasConceptScore W3199892757C133571119 @default.