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- W3200345564 abstract "Abstract Background New approach for treating cancer is to influence on vital genes for tumors. A number of attempts have led to discovery candidate genes that are mean to be essential for surviving of tumor cells of different types. In this work, we tried to analyze genes that were previously found to be essential for melanoma surviving. We performed transient siRNA-mediated knockdown of UNC45A, STK11IP, RHPN2 and ZNFX1 in melanoma cell line A375. After that, we checked cell viability, proliferation and migrating rate in vitro. Methods For knockdown of the genes we used siRNA mediated interference. To deliver siRNA in cells we used transfection by Metafectene PRO. Cell viability and proliferation assessed by MTT assay. Cell migration was assessed by wound healing assay. Knockdown of genes was evaluated via RT-qPCR analysis. Results Our results indicate that knockdown of the genes leads to no statistically significant changes in cell survival according to viability assay. However, mobility assay shows that knockdown of each of the target genes accelerates the speed of cells migrating. Conclusion Our results do not confirm the hypothesis. Possible explaining of such controversial results could be in insufficient bioinformatical analysis or in need of multiplex knockdown of the genes." @default.
- W3200345564 created "2021-09-27" @default.
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- W3200345564 date "2020-07-17" @default.
- W3200345564 modified "2023-10-18" @default.
- W3200345564 title "Analysis Of Candidate Genes Expected To Be Essential For Melanoma Surviving" @default.
- W3200345564 doi "https://doi.org/10.21203/rs.3.rs-43507/v1" @default.
- W3200345564 hasPublicationYear "2020" @default.
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