Matches in SemOpenAlex for { <https://semopenalex.org/work/W3200364733> ?p ?o ?g. }
- W3200364733 endingPage "43" @default.
- W3200364733 startingPage "30" @default.
- W3200364733 abstract "Viral infections and reactivations still remain a cause of morbidity and mortality after hematopoietic stem cell transplantation due to immunodeficiency and immunosuppression. Transfer of unmanipulated donor-derived lymphocytes (DLI) represents a promising strategy for improving cellular immunity but carries the risk of graft versus host disease (GvHD). Depleting alloreactive naïve T cells (TN) from DLIs was implemented to reduce the risk of GvHD induction while preserving antiviral memory T-cell activity. Here, we compared two TN depletion strategies via CD45RA and CD62L expression and investigated the presence of antiviral memory T cells against human adenovirus (AdV) and Epstein-Barr virus (EBV) in the depleted fractions in relation to their functional and immunophenotypic characteristics.T-cell responses against ppEBV_EBNA1, ppEBV_Consensus and ppAdV_Hexon within TN-depleted (CD45RA-/CD62L-) and TN-enriched (CD45RA+/CD62L+) fractions were quantified by interferon-gamma (IFN-γ) ELISpot assay after short- and long-term in vitro stimulation. T-cell frequencies and immunophenotypic composition were assessed in all fractions by flow cytometry. Moreover, alloimmune T-cell responses were evaluated by mixed lymphocyte reaction.According to differences in the phenotype composition, antigen-specific T-cell responses in CD45RA- fraction were up to 2 times higher than those in the CD62L- fraction, with the highest increase (up to 4-fold) observed after 7 days for ppEBV_EBNA1-specific T cells. The CD4+ effector memory T cells (TEM) were mainly responsible for EBV_EBNA1- and AdV_Hexon-specific T-cell responses, whereas the main functionally active T cells against ppEBV_Consensus were CD8+ central memory T cells (TCM) and TEM. Moreover, comparison of both depletion strategies indicated that alloreactivity in CD45RA- was lower than that in CD62L- fraction.Taken together, our results indicate that CD45RA depletion is a more suitable strategy for generating TN-depleted products consisting of memory T cells against ppEBV_EBNA1 and ppAdV_Hexon than CD62L in terms of depletion effectiveness, T-cell functionality and alloreactivity. To maximally exploit the beneficial effects mediated by antiviral memory T cells in TN-depleted products, depletion methods should be selected individually according to phenotype composition and CD4/CD8 antigen restriction. TN-depleted DLIs may improve the clinical outcome in terms of infections, GvHD, and disease relapse if selection of pathogen-specific donor T cells is not available." @default.
- W3200364733 created "2021-09-27" @default.
- W3200364733 creator A5002173254 @default.
- W3200364733 creator A5010671189 @default.
- W3200364733 creator A5022332487 @default.
- W3200364733 creator A5025862786 @default.
- W3200364733 creator A5034000100 @default.
- W3200364733 creator A5057354322 @default.
- W3200364733 creator A5077508029 @default.
- W3200364733 creator A5080762423 @default.
- W3200364733 date "2021-09-10" @default.
- W3200364733 modified "2023-10-18" @default.
- W3200364733 title "Variances in Antiviral Memory T-Cell Repertoire of CD45RA- and CD62L-Depleted Lymphocyte Products Reflect the Need of Individual T-Cell Selection Strategies to Reduce the Risk of GvHD while Preserving Antiviral Immunity in Adoptive T-Cell Therapy" @default.
- W3200364733 cites W1562712212 @default.
- W3200364733 cites W1574493485 @default.
- W3200364733 cites W1590531419 @default.
- W3200364733 cites W1630218117 @default.
- W3200364733 cites W1677866304 @default.
- W3200364733 cites W1835452576 @default.
- W3200364733 cites W1959270830 @default.
- W3200364733 cites W1981480494 @default.
- W3200364733 cites W1981690705 @default.
- W3200364733 cites W1982041503 @default.
- W3200364733 cites W1983062149 @default.
- W3200364733 cites W1984891922 @default.
- W3200364733 cites W1994340919 @default.
- W3200364733 cites W2000108150 @default.
- W3200364733 cites W2005788976 @default.
- W3200364733 cites W2006097708 @default.
- W3200364733 cites W2008788809 @default.
- W3200364733 cites W2015899626 @default.
- W3200364733 cites W2028568444 @default.
- W3200364733 cites W2033477706 @default.
- W3200364733 cites W2044329467 @default.
- W3200364733 cites W2052012700 @default.
- W3200364733 cites W2055523490 @default.
- W3200364733 cites W2058001723 @default.
- W3200364733 cites W2068699102 @default.
- W3200364733 cites W2071989019 @default.
- W3200364733 cites W2073045132 @default.
- W3200364733 cites W2073151752 @default.
- W3200364733 cites W2073740449 @default.
- W3200364733 cites W2074640102 @default.
- W3200364733 cites W2078390252 @default.
- W3200364733 cites W2080094805 @default.
- W3200364733 cites W2082614323 @default.
- W3200364733 cites W2086928462 @default.
- W3200364733 cites W2092080772 @default.
- W3200364733 cites W2093078925 @default.
- W3200364733 cites W2093964518 @default.
- W3200364733 cites W2097855813 @default.
- W3200364733 cites W2106153683 @default.
- W3200364733 cites W2110255986 @default.
- W3200364733 cites W2113969422 @default.
- W3200364733 cites W2128571215 @default.
- W3200364733 cites W2130113751 @default.
- W3200364733 cites W2134076328 @default.
- W3200364733 cites W2134201306 @default.
- W3200364733 cites W2134916334 @default.
- W3200364733 cites W2135330281 @default.
- W3200364733 cites W2143015280 @default.
- W3200364733 cites W2145928191 @default.
- W3200364733 cites W2149347577 @default.
- W3200364733 cites W2153666482 @default.
- W3200364733 cites W2156026365 @default.
- W3200364733 cites W2161841913 @default.
- W3200364733 cites W2162457545 @default.
- W3200364733 cites W2163275132 @default.
- W3200364733 cites W2164277825 @default.
- W3200364733 cites W2165712375 @default.
- W3200364733 cites W2170203516 @default.
- W3200364733 cites W2171841416 @default.
- W3200364733 cites W2175592917 @default.
- W3200364733 cites W2291114217 @default.
- W3200364733 cites W2338603660 @default.
- W3200364733 cites W2761374724 @default.
- W3200364733 cites W2761617108 @default.
- W3200364733 cites W2769044088 @default.
- W3200364733 cites W2777561030 @default.
- W3200364733 cites W2784031161 @default.
- W3200364733 cites W2791297313 @default.
- W3200364733 cites W2914723032 @default.
- W3200364733 cites W2923571241 @default.
- W3200364733 cites W2951576316 @default.
- W3200364733 cites W2970402231 @default.
- W3200364733 cites W3000206567 @default.
- W3200364733 cites W3005910409 @default.
- W3200364733 cites W3040015203 @default.
- W3200364733 cites W2263755637 @default.
- W3200364733 doi "https://doi.org/10.1159/000516284" @default.
- W3200364733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35221866" @default.
- W3200364733 hasPublicationYear "2021" @default.
- W3200364733 type Work @default.
- W3200364733 sameAs 3200364733 @default.
- W3200364733 citedByCount "2" @default.
- W3200364733 countsByYear W32003647332023 @default.
- W3200364733 crossrefType "journal-article" @default.
- W3200364733 hasAuthorship W3200364733A5002173254 @default.