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• W3200438706 abstract "Psoriasis is a chronic inflammatory skin disease involving several cell types, including T cells, via the IL-23/IL-17 axis. IL-17A acts on the surrounding epithelial cells thus resulting in an inflammatory feedback loop. The development of immunocompetent models that correctly recapitulate the complex phenotype of psoriasis remains challenging, which also includes both the T cell isolation and activation methods. The purpose of this work was to develop an advanced in vitro 3D psoriatic skin model that enables the study of the impact of T cells on psoriatic epithelial cells. To reach that aim, healthy and psoriatic fibroblasts and keratinocytes were used to reproduce this tissue-engineered skin model in which activated T cells, isolated beforehand from human whole blood, have been incorporated. Our study showed that isolation of T cells with the EasySep procedure, followed by activation with PMA/ionomycin, mimicked the psoriatic characteristics in an optimal manner with the production of inflammatory cytokines important in the pathogenesis of psoriasis, as well as increased expression of Ki67, S100A7, elafin and involucrin. This psoriatic model enriched in activated T cells displayed enhanced production of IL-17A, IFN-ƴ, CCL2, CXCL10, IL-1ra, IL-6 and CXCL8 compared with the healthy model and whose increased secretion was maintained over time. In addition, anti-IL17A treatment restored some psoriatic features, including epidermal thickness and basal keratinocytes proliferation, as well as a downregulation of S100A7, elafin and involucrin expression. Altogether, our study demonstrated that this model reflects a proper psoriatic inflammatory environment and is effective for the investigation of epidermal and T cell interaction over time. STATEMENT OF SIGNIFICANCE: The aim of this study was to provide an innovative 3D immunocompetent human psoriatic skin model. To our knowledge, this is the first immunocompetent model that uses skin cells from psoriatic patients to study the impact of IL-17A on pathological cells. Through the use of this model, we demonstrated that the T-cell enriched psoriatic model differs from T-cell enriched healthy model, highlighting efficient crosstalk between pathologic epithelial cells and T cells. This advanced preclinical model further mimics the original psoriatic skin and will prove relevant in predicting clinical outcomes, thereby decreasing inaccurate predictions of compound effects." @default.
• W3200438706 created "2021-09-27" @default.
• W3200438706 creator A5032116731 @default.
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• W3200438706 date "2021-12-01" @default.
• W3200438706 modified "2023-10-16" @default.
• W3200438706 title "Development of a 3D psoriatic skin model optimized for infiltration of IL-17A producing T cells: Focus on the crosstalk between T cells and psoriatic keratinocytes" @default.
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• W3200438706 cites W1969629598 @default.
• W3200438706 cites W1972872350 @default.
• W3200438706 cites W1973492077 @default.
• W3200438706 cites W1976306345 @default.
• W3200438706 cites W1976678898 @default.
• W3200438706 cites W1979962636 @default.
• W3200438706 cites W1980079027 @default.
• W3200438706 cites W1981177225 @default.
• W3200438706 cites W1982598880 @default.
• W3200438706 cites W1984846010 @default.
• W3200438706 cites W1986268987 @default.
• W3200438706 cites W1994315436 @default.
• W3200438706 cites W1996117691 @default.
• W3200438706 cites W1997267924 @default.
• W3200438706 cites W2006821600 @default.
• W3200438706 cites W2010795737 @default.
• W3200438706 cites W2020760332 @default.
• W3200438706 cites W2027976735 @default.
• W3200438706 cites W2039953294 @default.
• W3200438706 cites W2059311670 @default.
• W3200438706 cites W2061439388 @default.
• W3200438706 cites W2063854762 @default.
• W3200438706 cites W2067520762 @default.
• W3200438706 cites W2072719268 @default.
• W3200438706 cites W2080090843 @default.
• W3200438706 cites W2081545849 @default.
• W3200438706 cites W2082123274 @default.
• W3200438706 cites W2083370965 @default.
• W3200438706 cites W2093308972 @default.
• W3200438706 cites W2093785176 @default.
• W3200438706 cites W2101369041 @default.
• W3200438706 cites W2102889851 @default.
• W3200438706 cites W2103018315 @default.
• W3200438706 cites W2106247702 @default.
• W3200438706 cites W2109545029 @default.
• W3200438706 cites W2111037069 @default.
• W3200438706 cites W2118227917 @default.
• W3200438706 cites W2128156351 @default.
• W3200438706 cites W2145677448 @default.
• W3200438706 cites W2169996531 @default.
• W3200438706 cites W2325147761 @default.
• W3200438706 cites W2332870503 @default.
• W3200438706 cites W2499510596 @default.
• W3200438706 cites W2517997451 @default.
• W3200438706 cites W2567186204 @default.
• W3200438706 cites W2602063615 @default.
• W3200438706 cites W2622237865 @default.
• W3200438706 cites W2772067453 @default.
• W3200438706 cites W2775663182 @default.
• W3200438706 cites W2776126594 @default.
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• W3200438706 cites W2792797672 @default.
• W3200438706 cites W2883191655 @default.
• W3200438706 cites W2887422108 @default.
• W3200438706 cites W2894075989 @default.
• W3200438706 cites W2897926103 @default.
• W3200438706 cites W2908494584 @default.
• W3200438706 cites W2911246983 @default.
• W3200438706 cites W2926710375 @default.
• W3200438706 cites W2936636291 @default.
• W3200438706 cites W2939211834 @default.
• W3200438706 cites W2946942881 @default.
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• W3200438706 doi "https://doi.org/10.1016/j.actbio.2021.09.018" @default.
• W3200438706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34547515" @default.
• W3200438706 hasPublicationYear "2021" @default.
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