Matches in SemOpenAlex for { <https://semopenalex.org/work/W3200514646> ?p ?o ?g. }
- W3200514646 abstract "The preinitiation complex (PIC) for transcriptional initiation by RNA polymerase (Pol) II is composed of general transcription factors that are highly conserved. However, analysis of ChIP-seq datasets reveals kinetic and compositional differences in the transcriptional initiation process among eukaryotic species. In yeast, Mediator associates strongly with activator proteins bound to enhancers, but it transiently associates with promoters in a form that lacks the kinase module. In contrast, in human, mouse, and fly cells, Mediator with its kinase module stably associates with promoters, but not with activator-binding sites. This suggests that yeast and metazoans differ in the nature of the dynamic bridge of Mediator between activators and Pol II and the composition of a stable inactive PIC-like entity. As in yeast, occupancies of TATA-binding protein (TBP) and TBP-associated factors (Tafs) at mammalian promoters are not strictly correlated. This suggests that within PICs, TFIID is not a monolithic entity, and multiple forms of TBP affect initiation at different classes of genes. TFIID in flies, but not yeast and mammals, interacts strongly at regions downstream of the initiation site, consistent with the importance of downstream promoter elements in that species. Lastly, Taf7 and the mammalian-specific Med26 subunit of Mediator also interact near the Pol II pause region downstream of the PIC, but only in subsets of genes and often not together. Species-specific differences in PIC structure and function are likely to affect how activators and repressors affect transcriptional activity." @default.
- W3200514646 created "2021-09-27" @default.
- W3200514646 creator A5058594084 @default.
- W3200514646 creator A5083029357 @default.
- W3200514646 date "2021-09-13" @default.
- W3200514646 modified "2023-10-16" @default.
- W3200514646 title "Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species" @default.
- W3200514646 cites W1852931409 @default.
- W3200514646 cites W1974595249 @default.
- W3200514646 cites W1975950097 @default.
- W3200514646 cites W1984901090 @default.
- W3200514646 cites W1986156026 @default.
- W3200514646 cites W1992428272 @default.
- W3200514646 cites W1995251947 @default.
- W3200514646 cites W2006277271 @default.
- W3200514646 cites W2008619144 @default.
- W3200514646 cites W2010416554 @default.
- W3200514646 cites W2011626068 @default.
- W3200514646 cites W2014787267 @default.
- W3200514646 cites W2016786878 @default.
- W3200514646 cites W2020774845 @default.
- W3200514646 cites W2024093804 @default.
- W3200514646 cites W2026238009 @default.
- W3200514646 cites W2033317613 @default.
- W3200514646 cites W2035301928 @default.
- W3200514646 cites W2041348350 @default.
- W3200514646 cites W2042930277 @default.
- W3200514646 cites W2046109302 @default.
- W3200514646 cites W2053492846 @default.
- W3200514646 cites W2056304859 @default.
- W3200514646 cites W2060484997 @default.
- W3200514646 cites W2062015108 @default.
- W3200514646 cites W2062408930 @default.
- W3200514646 cites W2062630554 @default.
- W3200514646 cites W2064210252 @default.
- W3200514646 cites W2066881724 @default.
- W3200514646 cites W2067956561 @default.
- W3200514646 cites W2071273015 @default.
- W3200514646 cites W2078472628 @default.
- W3200514646 cites W2078578982 @default.
- W3200514646 cites W2085428320 @default.
- W3200514646 cites W2087400406 @default.
- W3200514646 cites W2088345878 @default.
- W3200514646 cites W2091533196 @default.
- W3200514646 cites W2094563726 @default.
- W3200514646 cites W2095771622 @default.
- W3200514646 cites W2097009498 @default.
- W3200514646 cites W2105028372 @default.
- W3200514646 cites W2117792884 @default.
- W3200514646 cites W2125815477 @default.
- W3200514646 cites W2137300752 @default.
- W3200514646 cites W2144688273 @default.
- W3200514646 cites W2149223106 @default.
- W3200514646 cites W2149920358 @default.
- W3200514646 cites W2159518929 @default.
- W3200514646 cites W2161671616 @default.
- W3200514646 cites W2164154631 @default.
- W3200514646 cites W2167389868 @default.
- W3200514646 cites W2168602111 @default.
- W3200514646 cites W2170005506 @default.
- W3200514646 cites W2175853567 @default.
- W3200514646 cites W2217471163 @default.
- W3200514646 cites W2273790193 @default.
- W3200514646 cites W2308896320 @default.
- W3200514646 cites W2314067827 @default.
- W3200514646 cites W2532168152 @default.
- W3200514646 cites W2536547099 @default.
- W3200514646 cites W2562186464 @default.
- W3200514646 cites W2592853334 @default.
- W3200514646 cites W2615878271 @default.
- W3200514646 cites W2742189960 @default.
- W3200514646 cites W2745090396 @default.
- W3200514646 cites W2746992081 @default.
- W3200514646 cites W2752525798 @default.
- W3200514646 cites W2763578259 @default.
- W3200514646 cites W2889409921 @default.
- W3200514646 cites W2911925591 @default.
- W3200514646 cites W2942896967 @default.
- W3200514646 cites W2945278782 @default.
- W3200514646 cites W2950673625 @default.
- W3200514646 cites W2967977322 @default.
- W3200514646 cites W2990523642 @default.
- W3200514646 cites W2994069347 @default.
- W3200514646 cites W3001272727 @default.
- W3200514646 cites W3002889477 @default.
- W3200514646 cites W3045910546 @default.
- W3200514646 cites W3089290065 @default.
- W3200514646 cites W3189598002 @default.
- W3200514646 cites W4232123327 @default.
- W3200514646 cites W4241487010 @default.
- W3200514646 cites W4243329727 @default.
- W3200514646 cites W4244866225 @default.
- W3200514646 cites W4378744478 @default.
- W3200514646 doi "https://doi.org/10.7554/elife.67964" @default.
- W3200514646 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8463073" @default.
- W3200514646 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34515029" @default.
- W3200514646 hasPublicationYear "2021" @default.
- W3200514646 type Work @default.
- W3200514646 sameAs 3200514646 @default.
- W3200514646 citedByCount "9" @default.