FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3200700251> ?p ?o ?g. }

• W3200700251 abstract "Abstract Campylobacter jejuni and Campylobacter coli were previously considered asaccharolytic, but are now known to possess specific saccharides metabolization pathways, including L-fucose. To investigate the influence of the L-fucose utilization cluster on Campylobacter growth, survival and metabolism, we performed comparative genotyping and phenotyping of the C. jejuni reference isolate NCTC11168 (human isolate), C. jejuni Ca1352 (chicken meat isolate), C. jejuni Ca2426 (sheep isolate), and C. coli Ca0121 (pig manure isolate), that all possess the L-fucose utilization cluster. All isolates showed enhanced survival and prolonged spiral cell morphology in aging cultures up to day seven in L-fucose-enriched MEMα medium (MEMαF) compared to MEMα. HPLC analysis indicated L-fucose utilization linked to acetate, lactate, pyruvate and succinate production, confirming the activation of the L-fucose pathway in these isolates. Highest consumption of L-fucose by C. coli Ca0121, is conceivably linked to its enhanced growth performance up to day 7, reaching 9.3 log CFU/ml compared to approximately 8.3 log CFU/ml for the C. jejuni isolates. Genetic analysis of their respective L-fucose clusters revealed several differences, including a 1 bp deletion in the Cj0489 gene of C. jejuni NCTC11168, causing a frameshift in this isolate resulting in two separate genes, Cj0489 and Cj0490 , while no apparent phenotype could be linked to the presumed frameshift in the NCTC11168 isolate. Additionally, we found that the L-fucose cluster of C. coli Ca0121 was most distant from C. jejuni NCTC11168, but confirmation of links to L-fucose metabolism associated phenotypic traits in C. coli versus C. jejuni isolates requires further studies. Importance Campylobacter is the leading cause of gastroenteritis in humans worldwide, with increasing incidence and prevalence in recent years. The most prevalent species are Campylobacter jejuni and C. coli with 83% and 10% of all Campylobacter cases, respectively. Previously it was found that the majority of Campylobacter isolates are able to metabolize L-fucose (fuc+ isolates), a sugar that is widely present in the human gut. Putative roles for L-fucose in fuc+ C. jejuni isolates were found in growth, biofilm formation and virulence. Despite this, relatively little is known about L-fucose metabolism and the impact on growth and survival in fuc+ Campylobacter isolates. The results from our comparative genotyping and phenotyping study demonstrate that L-fucose, in both C. jejuni and C. coli fuc+ isolates, is involved in enhanced survival, prolonged spiral cell morphology and changes in the general metabolism. Possible links between phenotypes and differences in respective L-fucose gene clusters are discussed." @default.
• W3200700251 created "2021-09-27" @default.
• W3200700251 creator A5016731724 @default.
• W3200700251 creator A5042804725 @default.
• W3200700251 creator A5051953333 @default.
• W3200700251 creator A5077542098 @default.
• W3200700251 creator A5090226743 @default.
• W3200700251 date "2021-09-10" @default.
• W3200700251 modified "2023-09-27" @default.
• W3200700251 title "Comparative analysis of L-fucose utilization and its impact on growth and survival of <i>Campylobacter</i> isolates" @default.
• W3200700251 cites W1581010761 @default.
• W3200700251 cites W1849301422 @default.
• W3200700251 cites W1964214446 @default.
• W3200700251 cites W1964632527 @default.
• W3200700251 cites W1969849347 @default.
• W3200700251 cites W1970418859 @default.
• W3200700251 cites W1977060309 @default.
• W3200700251 cites W1985428531 @default.
• W3200700251 cites W2005458561 @default.
• W3200700251 cites W2020332632 @default.
• W3200700251 cites W2030336958 @default.
• W3200700251 cites W2049140820 @default.
• W3200700251 cites W2049767516 @default.
• W3200700251 cites W2062091988 @default.
• W3200700251 cites W2065554192 @default.
• W3200700251 cites W2073449104 @default.
• W3200700251 cites W2077514992 @default.
• W3200700251 cites W2095680279 @default.
• W3200700251 cites W2098289841 @default.
• W3200700251 cites W2100920846 @default.
• W3200700251 cites W2104293843 @default.
• W3200700251 cites W2115712288 @default.
• W3200700251 cites W2117484859 @default.
• W3200700251 cites W2117946498 @default.
• W3200700251 cites W2127214408 @default.
• W3200700251 cites W2133676159 @default.
• W3200700251 cites W2135973921 @default.
• W3200700251 cites W2139739054 @default.
• W3200700251 cites W2140350620 @default.
• W3200700251 cites W2150180261 @default.
• W3200700251 cites W2155053954 @default.
• W3200700251 cites W2168498520 @default.
• W3200700251 cites W2313469424 @default.
• W3200700251 cites W2332864286 @default.
• W3200700251 cites W2346707626 @default.
• W3200700251 cites W2484771392 @default.
• W3200700251 cites W2522680429 @default.
• W3200700251 cites W2558388956 @default.
• W3200700251 cites W2595451031 @default.
• W3200700251 cites W2616355258 @default.
• W3200700251 cites W2783262056 @default.
• W3200700251 cites W2891593203 @default.
• W3200700251 cites W2897295876 @default.
• W3200700251 cites W2916419311 @default.
• W3200700251 cites W2961726900 @default.
• W3200700251 cites W3003690006 @default.
• W3200700251 cites W3021442976 @default.
• W3200700251 cites W3107434498 @default.
• W3200700251 cites W4240236037 @default.
• W3200700251 cites W4244464723 @default.
• W3200700251 cites W4294350218 @default.
• W3200700251 cites W4376848200 @default.
• W3200700251 doi "https://doi.org/10.1101/2021.09.09.459711" @default.
• W3200700251 hasPublicationYear "2021" @default.
• W3200700251 type Work @default.
• W3200700251 sameAs 3200700251 @default.
• W3200700251 citedByCount "0" @default.
• W3200700251 crossrefType "posted-content" @default.
• W3200700251 hasAuthorship W3200700251A5016731724 @default.
• W3200700251 hasAuthorship W3200700251A5042804725 @default.
• W3200700251 hasAuthorship W3200700251A5051953333 @default.
• W3200700251 hasAuthorship W3200700251A5077542098 @default.
• W3200700251 hasAuthorship W3200700251A5090226743 @default.
• W3200700251 hasBestOaLocation W32007002511 @default.
• W3200700251 hasConcept C105702510 @default.
• W3200700251 hasConcept C2776841590 @default.
• W3200700251 hasConcept C2776957140 @default.
• W3200700251 hasConcept C2778022156 @default.
• W3200700251 hasConcept C2778173381 @default.
• W3200700251 hasConcept C2779910956 @default.
• W3200700251 hasConcept C523546767 @default.
• W3200700251 hasConcept C54355233 @default.
• W3200700251 hasConcept C55493867 @default.
• W3200700251 hasConcept C86803240 @default.
• W3200700251 hasConcept C89423630 @default.
• W3200700251 hasConceptScore W3200700251C105702510 @default.
• W3200700251 hasConceptScore W3200700251C2776841590 @default.
• W3200700251 hasConceptScore W3200700251C2776957140 @default.
• W3200700251 hasConceptScore W3200700251C2778022156 @default.
• W3200700251 hasConceptScore W3200700251C2778173381 @default.
• W3200700251 hasConceptScore W3200700251C2779910956 @default.
• W3200700251 hasConceptScore W3200700251C523546767 @default.
• W3200700251 hasConceptScore W3200700251C54355233 @default.
• W3200700251 hasConceptScore W3200700251C55493867 @default.
• W3200700251 hasConceptScore W3200700251C86803240 @default.
• W3200700251 hasConceptScore W3200700251C89423630 @default.
• W3200700251 hasLocation W32007002511 @default.
• W3200700251 hasLocation W32007002512 @default.
• W3200700251 hasLocation W32007002513 @default.
• W3200700251 hasOpenAccess W3200700251 @default.

• next