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• W3200803985 startingPage "113618" @default.
• W3200803985 abstract "Carcinoembryonic antigen (CEA) is an important malign tumor marker. In this study, a simple, label-free and antibody-free aptasensor was fabricated based on a multifunctional dendrimer-like DNA nanoassembly. The DNA nanoassembly was embedded with multiple G-quadruplex DNAzyme motifs and a hanging CEA aptamer motif. It was prepared from short DNA sequences by autonomous-assembly. The aptasensor was prepared simply by self-assembly of a capture DNA (cpDNA) on a gold electrode, followed by hybridization with a CEA aptamer (AptGAC-P). CEA as a model target was detected through competitive binding of CEA with AptGAC-P, exposing cpDNA to bind with the DNA nanoassembly. The detection process only contains 2 incubation steps. The high load of G-quadruplex DNAzyme motifs and their catalytic activity resulted in an amplified and label-free differential pulse voltammetry (DPV) electrochemical signal. The peak current correlated linearly with the CEA concentration, with a linear range of 2–45 ng mL −1 , and an LOD value of 0.24 ng mL −1 . The aptasensor showed high specificity and reproducibility, and retained 96.5% of detection signal intensities after 31 days of storage. The recovery rates for spiked CEA in human serum were within 100 ± 5%, and the coincidence rates for clinical human serum samples with ELISA kits were 80.7–111%. Conceivably, possessing simplicity, sensitivity, reproducibility, storage stability, and accuracy, the aptasensor should be a very prominent and applicable tool for clinical CEA detection and cancer diagnosis, and is promisingly applicable as a platform for detecting other targets of interests. • A label-free aptasensor was fabricated based on multifunctional DNA nanoassembly. • The embedded multiple G-quadruplex DNAzyme motifs amplify the CEA detection signal. • The aptasensor was prepared by cpDNA immobilization and then aptamer hybridization. • The LOD value was 0.24 ng mL −1 , and signal intensities retained 96.5% after storage. • The recovery rates were within 100 ± 5%, and the coincidence rates were 80.7–111.0%." @default.
• W3200803985 created "2021-09-27" @default.
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• W3200803985 date "2021-12-01" @default.
• W3200803985 modified "2023-09-23" @default.
• W3200803985 title "A DNAzyme-catalyzed label-free aptasensor based on multifunctional dendrimer-like DNA assembly for sensitive detection of carcinoembryonic antigen" @default.
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• W3200803985 doi "https://doi.org/10.1016/j.bios.2021.113618" @default.
• W3200803985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34530373" @default.
• W3200803985 hasPublicationYear "2021" @default.
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