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- W3200808216 abstract "DNA double-strand breaks (DSB) are the most severe type of DNA damage. Despite the catastrophic consequences on genome integrity, it remains so far elusive how DSBs affect transcription. A reason for this was the lack of suitable tools to simultaneously monitor transcription and the induction of a genic DSB with sufficient temporal and spatial resolution. This work describes a set of new reporters that directly visualize transcription in live cells immediately after the induction of a DSB in the DNA template. Bacteriophage RNA stem-loops are employed to monitor the transcription with single-molecule sensitivity. For targetting the DSB to a specific gene region, the reporter genes are engineered to contain a single recognition sequence of the homing endonuclease I-SceI, otherwise absent from the human genome. A single copy of each reporter gene was integrated into the genome of human cell lines. This experimental system allows the detection of single RNA molecules generated by the canonical gene transcription or by DNA break-induced transcription initiation. These reporters provide an unprecedented opportunity for interpreting the reciprocal interactions between transcription and DNA damage and to disclose hitherto unappreciated aspects of DNA break-induced transcription." @default.
- W3200808216 created "2021-09-27" @default.
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- W3200808216 date "2021-09-20" @default.
- W3200808216 modified "2023-10-12" @default.
- W3200808216 title "Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks" @default.
- W3200808216 doi "https://doi.org/10.3791/62968" @default.
- W3200808216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34605818" @default.
- W3200808216 hasPublicationYear "2021" @default.
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