Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201007935> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W3201007935 endingPage "100780" @default.
- W3201007935 startingPage "100780" @default.
- W3201007935 abstract "Patient-derived organoids (PDOs) recapitulate the cellular heterogeneity of the original colorectal tumor tissue. Here, we describe a protocol to generate genetically modified PDOs to investigate cancer stem cells. This protocol uses the CRISPR-Cas9 system to knock-in the IRES-EGFP-P2A-iCaspase9 cassette into the 3' UTR of the potential cancer stem cell marker gene, which allows us to investigate their potential for self-replication and pluripotency. We describe the procedure for generating mutant PDOs and their application for stem cell research. For complete details on the generation and use of this protocol, please refer to Okamoto et al. Okamoto et al. (2021)." @default.
- W3201007935 created "2021-09-27" @default.
- W3201007935 creator A5003624452 @default.
- W3201007935 creator A5048106870 @default.
- W3201007935 creator A5055689145 @default.
- W3201007935 creator A5075969194 @default.
- W3201007935 creator A5087451913 @default.
- W3201007935 date "2021-12-01" @default.
- W3201007935 modified "2023-10-18" @default.
- W3201007935 title "A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids" @default.
- W3201007935 cites W2103385495 @default.
- W3201007935 cites W2159582900 @default.
- W3201007935 cites W2550079706 @default.
- W3201007935 cites W2914163908 @default.
- W3201007935 cites W3133682526 @default.
- W3201007935 cites W4234128816 @default.
- W3201007935 doi "https://doi.org/10.1016/j.xpro.2021.100780" @default.
- W3201007935 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8455475" @default.
- W3201007935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34585151" @default.
- W3201007935 hasPublicationYear "2021" @default.
- W3201007935 type Work @default.
- W3201007935 sameAs 3201007935 @default.
- W3201007935 citedByCount "8" @default.
- W3201007935 countsByYear W32010079352022 @default.
- W3201007935 countsByYear W32010079352023 @default.
- W3201007935 crossrefType "journal-article" @default.
- W3201007935 hasAuthorship W3201007935A5003624452 @default.
- W3201007935 hasAuthorship W3201007935A5048106870 @default.
- W3201007935 hasAuthorship W3201007935A5055689145 @default.
- W3201007935 hasAuthorship W3201007935A5075969194 @default.
- W3201007935 hasAuthorship W3201007935A5087451913 @default.
- W3201007935 hasBestOaLocation W32010079351 @default.
- W3201007935 hasConcept C104317684 @default.
- W3201007935 hasConcept C121608353 @default.
- W3201007935 hasConcept C144501496 @default.
- W3201007935 hasConcept C2796436 @default.
- W3201007935 hasConcept C28328180 @default.
- W3201007935 hasConcept C31695470 @default.
- W3201007935 hasConcept C502942594 @default.
- W3201007935 hasConcept C526805850 @default.
- W3201007935 hasConcept C54355233 @default.
- W3201007935 hasConcept C55427017 @default.
- W3201007935 hasConcept C70721500 @default.
- W3201007935 hasConcept C86803240 @default.
- W3201007935 hasConcept C98108389 @default.
- W3201007935 hasConceptScore W3201007935C104317684 @default.
- W3201007935 hasConceptScore W3201007935C121608353 @default.
- W3201007935 hasConceptScore W3201007935C144501496 @default.
- W3201007935 hasConceptScore W3201007935C2796436 @default.
- W3201007935 hasConceptScore W3201007935C28328180 @default.
- W3201007935 hasConceptScore W3201007935C31695470 @default.
- W3201007935 hasConceptScore W3201007935C502942594 @default.
- W3201007935 hasConceptScore W3201007935C526805850 @default.
- W3201007935 hasConceptScore W3201007935C54355233 @default.
- W3201007935 hasConceptScore W3201007935C55427017 @default.
- W3201007935 hasConceptScore W3201007935C70721500 @default.
- W3201007935 hasConceptScore W3201007935C86803240 @default.
- W3201007935 hasConceptScore W3201007935C98108389 @default.
- W3201007935 hasFunder F4320320912 @default.
- W3201007935 hasFunder F4320334764 @default.
- W3201007935 hasIssue "4" @default.
- W3201007935 hasLocation W32010079351 @default.
- W3201007935 hasLocation W32010079352 @default.
- W3201007935 hasLocation W32010079353 @default.
- W3201007935 hasLocation W32010079354 @default.
- W3201007935 hasLocation W32010079355 @default.
- W3201007935 hasOpenAccess W3201007935 @default.
- W3201007935 hasPrimaryLocation W32010079351 @default.
- W3201007935 hasRelatedWork W2403465893 @default.
- W3201007935 hasRelatedWork W2582314111 @default.
- W3201007935 hasRelatedWork W2594626182 @default.
- W3201007935 hasRelatedWork W2943124855 @default.
- W3201007935 hasRelatedWork W2998853150 @default.
- W3201007935 hasRelatedWork W3009070575 @default.
- W3201007935 hasRelatedWork W3010735555 @default.
- W3201007935 hasRelatedWork W3118208816 @default.
- W3201007935 hasRelatedWork W3185589017 @default.
- W3201007935 hasRelatedWork W4255099211 @default.
- W3201007935 hasVolume "2" @default.
- W3201007935 isParatext "false" @default.
- W3201007935 isRetracted "false" @default.
- W3201007935 magId "3201007935" @default.
- W3201007935 workType "article" @default.