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- W3201209218 abstract "Current methods for cryostorage in IVF clinics require manual retrieval of specimens from Dewars, canisters, canes, and goblets. The methods used for identification and retrieval of specimens are often not well characterized, and areas for improvement have gone unrealized regarding modernizing specimen cryostorage. The study included embryologists from four independent U.S. IVF clinics who were observed conducting clinical duties, under an IRB approved protocol, over an 8-week total period from August to November 2019. Embryologists were observed and video recorded while preforming routine daily tasks. Three measurable parameters were then documented in relation to the retrieval of viable specimens from storage Dewars: 1) the number of times the embryologist referenced the data record; 2) the number of times (pulls) the canister was lifted into the neck of the Dewar; and 3) the total time the canister was held in the neck. For each pull, a qualitative grade of 1 was assigned if the canister remained comfortably low in the neck of the Dewar; 2, if the canister was positioned close to the top of the neck; and 3, if the canister reached the top of the neck or came out of the Dewar. A total of 95 specimen retrievals were evaluated. A mean of 27.8±16.1 specimen retrievals were recorded per site. Subjects referred to the specimen data records 323 times; on average, it took 3.4 data record queries per retrieval to verify the identity of the cane. Of the 95 retrievals, 89 were from Dewars with necks. The canister was lifted an average of 1.5 times per retrieval, with a mean 11.8±9.2 seconds per pull, and a mean grade of 1.4±0.5. The minimum time for the canister remaining in the neck was 2 seconds with 50 seconds as the maximum. This study identified variance between the number of pulls and the time a canister spent in the neck of the Dewar, when retrieving cryopreserved specimens. Additional queries of the data demonstrated an increase in the time required to retrieve specimens. Eliminating the need for repeated manual review of data records, and more than one pull per retrieval, could help mitigate and lessen the opportunity for any mishandling of cryopreserved specimens. Due to the variability observed in this study, further investigation of both temperature fluctuations throughout specimen retrieval and the subsequent biological effect on the specimens is warranted." @default.
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- W3201209218 date "2021-09-01" @default.
- W3201209218 modified "2023-09-26" @default.
- W3201209218 title "A COLLABORATIVE ASSESSMENT TO MEASURE VARIANCE IN CRYOSPECIMEN RETRIEVAL METHODS ACROSS MULTIPLE IVF CLINICS" @default.
- W3201209218 doi "https://doi.org/10.1016/j.fertnstert.2021.07.1013" @default.
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