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• W3201219018 endingPage "106881" @default.
• W3201219018 startingPage "106881" @default.
• W3201219018 abstract "Sewage sludge bottom ash (SSBA) from the incineration plant used for the production of construction materials possibly possess heavy metals which might cause a negative impact on human health. Considering biosafety, we investigated the toxicity effects of 0.5-2 mm (aggregate substitute) and < 0.075 mm (cement substitute) in its solid and leachate form on human lung fibroblast cells (MRC-5) and human skin epidermal cells (HaCaT) on exposure through contact. MTS assay revealed the cellular responses of lung and skin cell lines to the leachates showing that the skin cells, which often interact with the external environment displayed better tolerance than the lung cells, whereas solid ash showed a concentration and size-dependent toxicity. Solid ash was found to downregulate the intracellular glutathione/superoxide dismutase activities and upregulate lactate dehydrogenase/lipid peroxidation activities thus inducing oxidative stress to the cell and subsequently resulting in the cell membrane leakage, destructive mitochondrial membrane potential (Δψm), apoptosis, and DNA damage, which is nearly 7-fold higher than the negative control. At a high concentration, DNA damage index of 1.09 and 1.29 was observed for the 0.5-2 mm sized ash leachate on skin cells and lung cells respectively, whereas for ash (<0.075 mm size) leachate, this fraction was 1.29 and 2.96, respectively. Overall, the ash leachate is found to be safer/biocompatible if they come in contact with humans as compared to SSBA in its solid form." @default.
• W3201219018 created "2021-09-27" @default.
• W3201219018 creator A5004160453 @default.
• W3201219018 creator A5023647985 @default.
• W3201219018 creator A5032958781 @default.
• W3201219018 creator A5040066940 @default.
• W3201219018 creator A5068113927 @default.
• W3201219018 creator A5088111577 @default.
• W3201219018 date "2022-01-01" @default.
• W3201219018 modified "2023-10-12" @default.
• W3201219018 title "Toxicity effects of size fractions of incinerated sewage sludge bottom ash on human cell lines" @default.
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