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- W3201315838 abstract "We have shown previously that PICSI is better in the clinical outcomes than the routine way of processing in cases of abnormal sperm DNA fragmentation (SDF). The objective to evaluate if selecting sperm from second ejaculate will do as good as PICSI with regard to the pre-implantation embryo development parameters in comparison to normal SDF group. Couples were included if the female age was ≤37 years, had a minimum of 5 mature oocytes (MII), and male with abnormal sperm DNA fragmentation (>20%) by TUNEL assay. On the day of ICSI, cases were randomized into two groups: (I) PICSI, where the first ejaculate is processed by double layer density gradient followed by PICSI dish for sperm selection, (II) Second ejaculate (1 hour after the first ejaculate), where the second ejaculate is processed by double layer density gradient only. We used a normal SDF group as a control along with the 2 comparative groups. There are no significant differences in the female age, male age, count, motility, SDF, smoking status, and no. of MII oocytes between the 3 groups. The study outcomes are shown in the table below.Tabled 1Point of comparisonNormal SDF (Control) n=91PICSI n=94Second ejaculate n=97P- valueFertilization rate (%)80.4±16.875.9±16.879.8±16.90.14Cleavage rate (%)79.8±17.375.1±17.279.2±17.10.12Blastocyst rate (%)66.8±21.465.1±21.665.1±23.60.75Good quality blastocyst rate (%)45.4±29.137.2±2941.8±280.15Fair quality blastocyst rate (%)15.2±17.816.9±19.227.3±380.22Bad quality blastocyst rate (%)32.5±2945.9±30.336.3±30.80.01*Data are mean ± SD. n refers to the number of patients for whom data were available. PICSI = physiological intracytoplasmic sperm injection. P values <0.05 are considered significant and marked with a * symbol. Open table in a new tab Second ejaculate seems to have comparable results to that of the PICSI and normal SDF groups, specially that it is a natural cost effective way to reduce SDF in cases with abnormal SDF undergoing ICSI. Future research studies should look if there is any significance in the clinical outcome before using it in the routine clinical scenarios." @default.
- W3201315838 created "2021-09-27" @default.
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- W3201315838 date "2021-09-01" @default.
- W3201315838 modified "2023-09-26" @default.
- W3201315838 title "STRATEGIES FOR REDUCING SPERM DNA FRAGMENTATION BY COMPARING PICSI VERSUS SECOND EJACULATE IN ICSI PATIENTS: A PROSPECTIVE RANDOMIZED CONTROLLED TRIAL" @default.
- W3201315838 doi "https://doi.org/10.1016/j.fertnstert.2021.07.752" @default.
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