Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201345877> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W3201345877 abstract "Abstract Background: T2DM (Type 2 diabetes) is a complex, chronic disease characterized as insulin resistance and islet β-cell dysfunction. Bariatric surgeries, mainly Roux-en-Y gastric bypass (RYGB) surgery and laparoscopic sleeve gastrectomy (LSG), have become an important method for treating obesity and T2DM, and GLP-1 increase after bariatric surgery has been regarded as a central event in bariatric surgery-induced remission of T2DM. Methods: The cell viability was examined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT). Cell apoptosis was determined by Flow cytometry. The cellular contents of cholesterol in INS-1 cells were measured using cholesterol assay kits. The BODIPY-cholesterol assay was performed to monitor the cholesterol efflux. Results: High concentration cholesterol-induced lipotoxicity was observed in INS-1 cells, including inhibited cell viability, enhanced cell apoptosis, and inhibited cholesterol efflux from INS-1 cells; in the meantime, ABCA1 protein level was decreased by cholesterol stimulation. Cholesterol-induced toxicity and ABCA1 downregulation was attenuated by GLP-1 agonist EX-4. GLP-1 induced AMPK phosphorylation during the protection against cholesterol-induced toxicity. Under cholesterol stimulation, GLP-1-induced AMPK activation inhibited PARP-1 activity, therefore attenuating cholesterol-induced toxicity in INS-1 cells. In INS-1 cells, PARP-1 directly interacted with LXR, leading to the poly(ADP-ribosyl)ation of LXRα and downregulation of LXR-mediated ABCA1 expression. In STZ-induced T2DM model in rats, RYGB surgery or EX-4 treatment improved the glucose metabolism and lipid metabolism in rats through GLP-1 inhibition of PARP-1 activity. According to clinical data, GLP-1 was significantly increased after LSG surgery on T2DM patients, and the increase in GLP-1 was closely related to the improvement of islet function. Conclusions: GLP-1 inhibits PARP-1 to protect islet β cell function against cholesterol-induced toxicity in vitro and in vivo through enhancing cholesterol efflux. GLP-1-induced AMPK and LXR-mediated ABCA1 expression are involved in GLP-1 protective effects." @default.
- W3201345877 created "2021-09-27" @default.
- W3201345877 creator A5005481695 @default.
- W3201345877 creator A5024722298 @default.
- W3201345877 creator A5029416027 @default.
- W3201345877 creator A5045870272 @default.
- W3201345877 creator A5058833050 @default.
- W3201345877 creator A5071488128 @default.
- W3201345877 creator A5075270486 @default.
- W3201345877 date "2020-03-03" @default.
- W3201345877 modified "2023-09-24" @default.
- W3201345877 title "GLP-1-induced AMPK activation inhibits PARP-1 and promotes LXR-mediated ABCA1 expression to protect pancreatic β-cells against cholesterol-induced toxicity through cholesterol efflux" @default.
- W3201345877 doi "https://doi.org/10.21203/rs.3.rs-15784/v1" @default.
- W3201345877 hasPublicationYear "2020" @default.
- W3201345877 type Work @default.
- W3201345877 sameAs 3201345877 @default.
- W3201345877 citedByCount "0" @default.
- W3201345877 crossrefType "posted-content" @default.
- W3201345877 hasAuthorship W3201345877A5005481695 @default.
- W3201345877 hasAuthorship W3201345877A5024722298 @default.
- W3201345877 hasAuthorship W3201345877A5029416027 @default.
- W3201345877 hasAuthorship W3201345877A5045870272 @default.
- W3201345877 hasAuthorship W3201345877A5058833050 @default.
- W3201345877 hasAuthorship W3201345877A5071488128 @default.
- W3201345877 hasAuthorship W3201345877A5075270486 @default.
- W3201345877 hasBestOaLocation W32013458771 @default.
- W3201345877 hasConcept C104317684 @default.
- W3201345877 hasConcept C11960822 @default.
- W3201345877 hasConcept C126322002 @default.
- W3201345877 hasConcept C127561419 @default.
- W3201345877 hasConcept C134018914 @default.
- W3201345877 hasConcept C149011108 @default.
- W3201345877 hasConcept C167400880 @default.
- W3201345877 hasConcept C185592680 @default.
- W3201345877 hasConcept C190283241 @default.
- W3201345877 hasConcept C2777391703 @default.
- W3201345877 hasConcept C2777704780 @default.
- W3201345877 hasConcept C2778163477 @default.
- W3201345877 hasConcept C2779306644 @default.
- W3201345877 hasConcept C2780124434 @default.
- W3201345877 hasConcept C44353895 @default.
- W3201345877 hasConcept C53227056 @default.
- W3201345877 hasConcept C55493867 @default.
- W3201345877 hasConcept C63932345 @default.
- W3201345877 hasConcept C71924100 @default.
- W3201345877 hasConcept C86339819 @default.
- W3201345877 hasConcept C86803240 @default.
- W3201345877 hasConcept C97029542 @default.
- W3201345877 hasConcept C98274493 @default.
- W3201345877 hasConceptScore W3201345877C104317684 @default.
- W3201345877 hasConceptScore W3201345877C11960822 @default.
- W3201345877 hasConceptScore W3201345877C126322002 @default.
- W3201345877 hasConceptScore W3201345877C127561419 @default.
- W3201345877 hasConceptScore W3201345877C134018914 @default.
- W3201345877 hasConceptScore W3201345877C149011108 @default.
- W3201345877 hasConceptScore W3201345877C167400880 @default.
- W3201345877 hasConceptScore W3201345877C185592680 @default.
- W3201345877 hasConceptScore W3201345877C190283241 @default.
- W3201345877 hasConceptScore W3201345877C2777391703 @default.
- W3201345877 hasConceptScore W3201345877C2777704780 @default.
- W3201345877 hasConceptScore W3201345877C2778163477 @default.
- W3201345877 hasConceptScore W3201345877C2779306644 @default.
- W3201345877 hasConceptScore W3201345877C2780124434 @default.
- W3201345877 hasConceptScore W3201345877C44353895 @default.
- W3201345877 hasConceptScore W3201345877C53227056 @default.
- W3201345877 hasConceptScore W3201345877C55493867 @default.
- W3201345877 hasConceptScore W3201345877C63932345 @default.
- W3201345877 hasConceptScore W3201345877C71924100 @default.
- W3201345877 hasConceptScore W3201345877C86339819 @default.
- W3201345877 hasConceptScore W3201345877C86803240 @default.
- W3201345877 hasConceptScore W3201345877C97029542 @default.
- W3201345877 hasConceptScore W3201345877C98274493 @default.
- W3201345877 hasLocation W32013458771 @default.
- W3201345877 hasLocation W32013458772 @default.
- W3201345877 hasOpenAccess W3201345877 @default.
- W3201345877 hasPrimaryLocation W32013458771 @default.
- W3201345877 hasRelatedWork W10537970 @default.
- W3201345877 hasRelatedWork W12876748 @default.
- W3201345877 hasRelatedWork W3657221 @default.
- W3201345877 hasRelatedWork W4364322 @default.
- W3201345877 hasRelatedWork W4980172 @default.
- W3201345877 hasRelatedWork W5932785 @default.
- W3201345877 hasRelatedWork W6495092 @default.
- W3201345877 hasRelatedWork W6525803 @default.
- W3201345877 hasRelatedWork W7621649 @default.
- W3201345877 hasRelatedWork W944767 @default.
- W3201345877 isParatext "false" @default.
- W3201345877 isRetracted "false" @default.
- W3201345877 magId "3201345877" @default.
- W3201345877 workType "article" @default.