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• W3201410663 abstract "Acrylamide (AA) is a food contaminant in thermally processed products that is object of tight control. A simple and easy-to-apply methodology for routine monitoring of AA levels in food products could allow producers to be players in the control of their own products. In this work, a simple methodology for AA quantification without derivatization was developed for biscuits, for which the benchmark levels recommended by EFSA are 350 µg/kg, and 150 µg/kg for biscuits for infants and young children. Headspace-solid phase microextraction (HS-SPME) was used in 120 mL screwed-cap vials with a carboxen/polydimetylsiloxane fiber, 4 g of biscuits, and 10 mL of water during 15 min at room temperature under stirring. The addition of 30 mL of propanol under stirring during 15 min at room temperature and 15 min at 60 °C was used to promote AA transfer to the headspace. The fiber exposure was 45 min. A gas chromatography-mass spectrometry analysis allowed to obtain an external calibration curve at m/z 71, with linearity R2 > 0.99 and precision RSD < 9%. The detection and quantification limits were 27.4 µg/kg and 91.5 µg/kg, respectively. The methodology was successfully used in biscuits with lower AA amount, where mitigation strategies (asparaginase or pectate) were applied." @default.
• W3201410663 created "2021-09-27" @default.
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• W3201410663 date "2021-09-14" @default.
• W3201410663 modified "2023-10-14" @default.
• W3201410663 title "HS-SPME Gas Chromatography Approach for Underivatized Acrylamide Determination in Biscuits" @default.
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• W3201410663 doi "https://doi.org/10.3390/foods10092183" @default.
• W3201410663 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8470632" @default.
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