FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W32014332> ?p ?o ?g. }

• W32014332 abstract "Everyday thousands of people suffer from bone diseases that lead to destruction of bone tissue. Various types of bone allograft, xenograft and synthetic biomaterial are now used for bone replacement therapy. The future of bone reconstruction lies in the use of stem cell technology for bone development. Within the bone marrow stroma there are exists a subset of nonhematopoietic cells referred to as mesenchymal stem cells (MSCs) or mesenchymal progenitor cells (MPCs). These cells can be expanded ex vivo and induced, either in vitro or in vivo, to terminally differentiate into osteoblasts, chondrocytes, adypocytes, tenocytes, myotubes, neural cells, and hematopoietic supporting stroma. The multipotential of these cells, their easy collection and culture, as well as their high ex vivo expansive potential makes these cells an attractive therapeutic tool. The purpose of this study was to isolate, expand and characterize mesenchymal stem cell from human bone marrow in Mesenchymal Stem Cell Growth Medium (MSCGM), to compare the cell growth in the MSCGM and Dulbecco Modified Eagle‟s Medium (DMEM) with 10% Fetal Bovine Serum (FBS) medium, to differentiate MSCs into osteoblast cells and to determine the osteogenic potential of the differentiated MSC by detecting the expression of osteoblast-specific genes such as collagen type 1, osteocalcin, runx 2, osteopontin and alkaline phosphatase. In this study, MSCs were isolated from human bone marrow and cultured in MSCGM medium and DMEM-10% FBS medium. Culture-expansion of MSCs was characterized by the presence of CD 105 marker using Labelled Streptavidin Biotin (LSAB) method. The MSCs were cultured in the MSCGM and DMEM-10% FBS medium within five days. The MSCs were then cultured in osteogenic medium containing DMEM medium supplemented with Fetal Bovine Serum (FBS), antibiotics, ascorbic acid, β-glycerol phosphate and dexamethasone to differentiate into osteoblasts. Their osteogenic differentiation was determined with the formation of a mineralized extracellular matrix visualized by Von Kossa staining and Alkaline Phosphatase (ALP) assay. Moreover, osteogenic differentiation was also judge by RT-PCR profiling of osteoblast gene expression. MSC first attached to the dish surface and exhibited fibroblast-like spindle shape. The cells were also identified as MSCs based on their immunophenotype of CD 105 which resulted in funchia coloured staining. The MSCs culture in MSCGM medium expanded and proliferated rapidly compared to MSCs culture in DMEM-10% FBS medium. Incubation of bone marrow-derived MSCs in the osteogenic medium for 3 weeks resulted in a dramatic increase in ALP activity and accumulation of calcium deposit, as assessed by ALP assay and Von Kossa staining, respectively. This osteogenic potential upon culture in osteogenic medium was further confirmed by the RT-PCR analysis where the expressions of osteoblast specific genes were confirmed by molecular weight produced on agarose gel. We conclude that MSCGM is the best choice for expanding and proliferating MSCs and suggest that MSC from bone marrow have pure osteogenic potential and have the capability to differentiate into osteoblast. This potential assures that bone marrow can be a legitimate source of MSCs for production of osteoblast which can be utilized in bone replacement therapy." @default.
• W32014332 created "2016-06-24" @default.
• W32014332 creator A5072270951 @default.
• W32014332 date "2007-01-01" @default.
• W32014332 modified "2023-09-24" @default.
• W32014332 title "Isolation And Characterization Of Mesenchymal Cells From Human Bone Marrow And Their Development Into Bone Cells" @default.
• W32014332 hasPublicationYear "2007" @default.
• W32014332 type Work @default.
• W32014332 sameAs 32014332 @default.
• W32014332 citedByCount "0" @default.
• W32014332 crossrefType "dissertation" @default.
• W32014332 hasAuthorship W32014332A5072270951 @default.
• W32014332 hasConcept C123012128 @default.
• W32014332 hasConcept C131075544 @default.
• W32014332 hasConcept C13685319 @default.
• W32014332 hasConcept C160160445 @default.
• W32014332 hasConcept C181199279 @default.
• W32014332 hasConcept C185592680 @default.
• W32014332 hasConcept C198826908 @default.
• W32014332 hasConcept C201750760 @default.
• W32014332 hasConcept C202751555 @default.
• W32014332 hasConcept C203014093 @default.
• W32014332 hasConcept C20875687 @default.
• W32014332 hasConcept C2778260815 @default.
• W32014332 hasConcept C2780007613 @default.
• W32014332 hasConcept C28328180 @default.
• W32014332 hasConcept C55493867 @default.
• W32014332 hasConcept C86803240 @default.
• W32014332 hasConcept C95444343 @default.
• W32014332 hasConceptScore W32014332C123012128 @default.
• W32014332 hasConceptScore W32014332C131075544 @default.
• W32014332 hasConceptScore W32014332C13685319 @default.
• W32014332 hasConceptScore W32014332C160160445 @default.
• W32014332 hasConceptScore W32014332C181199279 @default.
• W32014332 hasConceptScore W32014332C185592680 @default.
• W32014332 hasConceptScore W32014332C198826908 @default.
• W32014332 hasConceptScore W32014332C201750760 @default.
• W32014332 hasConceptScore W32014332C202751555 @default.
• W32014332 hasConceptScore W32014332C203014093 @default.
• W32014332 hasConceptScore W32014332C20875687 @default.
• W32014332 hasConceptScore W32014332C2778260815 @default.
• W32014332 hasConceptScore W32014332C2780007613 @default.
• W32014332 hasConceptScore W32014332C28328180 @default.
• W32014332 hasConceptScore W32014332C55493867 @default.
• W32014332 hasConceptScore W32014332C86803240 @default.
• W32014332 hasConceptScore W32014332C95444343 @default.
• W32014332 hasLocation W320143321 @default.
• W32014332 hasOpenAccess W32014332 @default.
• W32014332 hasPrimaryLocation W320143321 @default.
• W32014332 hasRelatedWork W2357075379 @default.
• W32014332 hasRelatedWork W2359855053 @default.
• W32014332 hasRelatedWork W2362498336 @default.
• W32014332 hasRelatedWork W2374482926 @default.
• W32014332 hasRelatedWork W2379898479 @default.
• W32014332 hasRelatedWork W2380871475 @default.
• W32014332 hasRelatedWork W2381447275 @default.
• W32014332 hasRelatedWork W2383172547 @default.
• W32014332 hasRelatedWork W2383959407 @default.
• W32014332 hasRelatedWork W2384710188 @default.
• W32014332 hasRelatedWork W2385019181 @default.
• W32014332 hasRelatedWork W2385674792 @default.
• W32014332 hasRelatedWork W2387524209 @default.
• W32014332 hasRelatedWork W2390425819 @default.
• W32014332 hasRelatedWork W2390501519 @default.
• W32014332 hasRelatedWork W2391850769 @default.
• W32014332 hasRelatedWork W2393905519 @default.
• W32014332 hasRelatedWork W2414835973 @default.
• W32014332 hasRelatedWork W2529732694 @default.
• W32014332 hasRelatedWork W3211739418 @default.
• W32014332 isParatext "false" @default.
• W32014332 isRetracted "false" @default.
• W32014332 magId "32014332" @default.
• W32014332 workType "dissertation" @default.