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- W3201464103 endingPage "365" @default.
- W3201464103 startingPage "354" @default.
- W3201464103 abstract "Significance: Fluorescent probes and mass spectrometry are the two most popular and complementary methods to quantify thiols in biological systems. In this review, we focus on the widely used and commercially available methods to detect and quantify thiols in living cells and the general approaches applied in mass spectrometry-based thiol quantification. We hope that this review can serve as a general guide for redox biologists who are interested in thiol species. Sulfur, one of the most important elements in living systems, contributes to every aspect of physiology and pathology. Thiols, including cysteine, homocysteine, glutathione, hydrogen sulfide, and hydropersulfides, are the main players in the redox biology system. Therefore, quantifying these thiol species in biological systems is one of the important steps to understand their roles in biology. Recent Advances: Fluorescent probes and mass spectrometry-based methods have been developed to detect and/or quantify thiols in biological systems. Mass spectrometry-based methods have been the gold standard for metabolite quantification in cells. Fluorescent probes can directly detect or quantify thiol species in living cells with spatial and temporal resolutions. Additionally, organelle-specific fluorescent probes have been widely developed. These two methods are complementary to each other. Critical Issues: Reliable quantification of thiol species using fluorescent probes remains challenging. Future Directions: When developing fluorescent probes, we suggest using both the fluorescent probes and mass spectrometry-based thiol quantification methods to cross-check the results. In addition, we call on chemical biologists to move beyond qualitative probes and focus on probes that can provide quantitative results in live cells. These quantitative measurements based on fluorescent probes should be validated with mass spectrometry-based methods. More importantly, chemical biologists should make their probes accessible to the biology end users. Regarding mass spectrometry-based methods, quantification of the derivatized thiol specifies should fit into the general metabolomics workflow. Antioxid. Redox Signal. 36, 354-365." @default.
- W3201464103 created "2021-09-27" @default.
- W3201464103 creator A5013585925 @default.
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- W3201464103 creator A5057083379 @default.
- W3201464103 creator A5069645037 @default.
- W3201464103 date "2022-02-01" @default.
- W3201464103 modified "2023-10-17" @default.
- W3201464103 title "Fluorescent Probes and Mass Spectrometry-Based Methods to Quantify Thiols in Biological Systems" @default.
- W3201464103 cites W1163687489 @default.
- W3201464103 cites W1592150753 @default.
- W3201464103 cites W1787354014 @default.
- W3201464103 cites W1885975534 @default.
- W3201464103 cites W1971048984 @default.
- W3201464103 cites W1975255371 @default.
- W3201464103 cites W1981354494 @default.
- W3201464103 cites W1995370236 @default.
- W3201464103 cites W2002611021 @default.
- W3201464103 cites W2017521727 @default.
- W3201464103 cites W2021143006 @default.
- W3201464103 cites W2022630875 @default.
- W3201464103 cites W2036278497 @default.
- W3201464103 cites W2040835025 @default.
- W3201464103 cites W2043037824 @default.
- W3201464103 cites W2043929237 @default.
- W3201464103 cites W2045070686 @default.
- W3201464103 cites W2055916841 @default.
- W3201464103 cites W2066080487 @default.
- W3201464103 cites W2066139235 @default.
- W3201464103 cites W2071919400 @default.
- W3201464103 cites W2071986088 @default.
- W3201464103 cites W2082248564 @default.
- W3201464103 cites W2082607647 @default.
- W3201464103 cites W2083739522 @default.
- W3201464103 cites W2089389631 @default.
- W3201464103 cites W2096258216 @default.
- W3201464103 cites W2099398906 @default.
- W3201464103 cites W2109458478 @default.
- W3201464103 cites W2109974675 @default.
- W3201464103 cites W2115598142 @default.
- W3201464103 cites W2116757923 @default.
- W3201464103 cites W2117497185 @default.
- W3201464103 cites W2119039450 @default.
- W3201464103 cites W2136271920 @default.
- W3201464103 cites W2141610173 @default.
- W3201464103 cites W2154414424 @default.
- W3201464103 cites W2157677632 @default.
- W3201464103 cites W2159398472 @default.
- W3201464103 cites W2161969251 @default.
- W3201464103 cites W2162645036 @default.
- W3201464103 cites W2163010727 @default.
- W3201464103 cites W2168024298 @default.
- W3201464103 cites W2203006631 @default.
- W3201464103 cites W2235420431 @default.
- W3201464103 cites W2237068061 @default.
- W3201464103 cites W2312564542 @default.
- W3201464103 cites W2323375094 @default.
- W3201464103 cites W2325288941 @default.
- W3201464103 cites W2405848654 @default.
- W3201464103 cites W2464917764 @default.
- W3201464103 cites W2551824955 @default.
- W3201464103 cites W2552043470 @default.
- W3201464103 cites W2598555284 @default.
- W3201464103 cites W2598958389 @default.
- W3201464103 cites W2606353696 @default.
- W3201464103 cites W2607697691 @default.
- W3201464103 cites W2728439750 @default.
- W3201464103 cites W2734464815 @default.
- W3201464103 cites W2745522568 @default.
- W3201464103 cites W2766780446 @default.
- W3201464103 cites W2769069971 @default.
- W3201464103 cites W2769297395 @default.
- W3201464103 cites W2782220253 @default.
- W3201464103 cites W2788549437 @default.
- W3201464103 cites W2789200606 @default.
- W3201464103 cites W2796331273 @default.
- W3201464103 cites W2799516388 @default.
- W3201464103 cites W2802985983 @default.
- W3201464103 cites W2803997671 @default.
- W3201464103 cites W2810415057 @default.
- W3201464103 cites W2892533751 @default.
- W3201464103 cites W2907974517 @default.
- W3201464103 cites W2914661481 @default.
- W3201464103 cites W2914969272 @default.
- W3201464103 cites W2922508951 @default.
- W3201464103 cites W2944183471 @default.
- W3201464103 cites W2944278437 @default.
- W3201464103 cites W2945135092 @default.
- W3201464103 cites W2950627161 @default.
- W3201464103 cites W2951027422 @default.
- W3201464103 cites W2967009964 @default.
- W3201464103 cites W2972936715 @default.
- W3201464103 cites W2985532389 @default.
- W3201464103 cites W3001177146 @default.
- W3201464103 cites W3007436058 @default.
- W3201464103 cites W3016013187 @default.