Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201504662> ?p ?o ?g. }
- W3201504662 endingPage "462544" @default.
- W3201504662 startingPage "462544" @default.
- W3201504662 abstract "• A new cell membranes affinity column was prepared. • An interesting assay was developed by using this affinity column. • Three active compounds were found from Atractylodes lancea . The cell membrane-coated monolithic column (CMMC) ligand fishing assay is an interesting approach set up for the study of natural products (NPs). NPs such as Atractylodes lancea contain many compounds. Traditional methods used to separate compounds and determine active compounds by pharmacological tests are time-consuming and inefficient. Therefore, an alternative method is required to determine active compounds in NPs. Here, white blood cells were broken, and the white blood cell membranes (WBCMs) were immobilized on the surface of a monolithic column to form a CMMC. The column was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and confocal laser scanning microscopy. Combined with gas chromatography/mass spectrometry (GC/MS), the CMMC was used to screen active compounds in Atractylodes lancea . Three potential active compounds including hinesol, β -eudesmol, and 4-phenylbenzaldehyde were discovered. A molecular docking assay demonstrated that these compounds could bind to MD-2 laid on WBCMs. In addition, antiinflammatory effects by the discovered compound in vitro were confirmed, and β -eudesmol showed a concentration-dependent inhibitory effect on the tumor necrosis factor (TNF)- α of a RAW264.7 cell ( P < 0.05). The CMMC ligand fishing assay exhibits good selectivity, great speed effects and is a potentially reliable tool for drug discovery in NPs." @default.
- W3201504662 created "2021-09-27" @default.
- W3201504662 creator A5000323275 @default.
- W3201504662 creator A5023990028 @default.
- W3201504662 creator A5041209710 @default.
- W3201504662 creator A5053447705 @default.
- W3201504662 creator A5091095745 @default.
- W3201504662 date "2021-10-01" @default.
- W3201504662 modified "2023-10-10" @default.
- W3201504662 title "Ligand fishing via a monolithic column coated with white blood cell membranes: A useful technique for screening active compounds in Astractylodes lancea" @default.
- W3201504662 cites W1541281484 @default.
- W3201504662 cites W1827411509 @default.
- W3201504662 cites W1926033671 @default.
- W3201504662 cites W1967459248 @default.
- W3201504662 cites W1968682237 @default.
- W3201504662 cites W1969592901 @default.
- W3201504662 cites W1980052502 @default.
- W3201504662 cites W1983799392 @default.
- W3201504662 cites W1992289682 @default.
- W3201504662 cites W1992411964 @default.
- W3201504662 cites W1993313156 @default.
- W3201504662 cites W2000133902 @default.
- W3201504662 cites W2006475885 @default.
- W3201504662 cites W2008945607 @default.
- W3201504662 cites W2020285181 @default.
- W3201504662 cites W2023176894 @default.
- W3201504662 cites W2024162840 @default.
- W3201504662 cites W2028226782 @default.
- W3201504662 cites W2033463617 @default.
- W3201504662 cites W2042932003 @default.
- W3201504662 cites W2044671489 @default.
- W3201504662 cites W2068855985 @default.
- W3201504662 cites W2070457128 @default.
- W3201504662 cites W2073856986 @default.
- W3201504662 cites W2074793023 @default.
- W3201504662 cites W2076661536 @default.
- W3201504662 cites W2079393314 @default.
- W3201504662 cites W2080298383 @default.
- W3201504662 cites W2086676026 @default.
- W3201504662 cites W2114953690 @default.
- W3201504662 cites W2146088771 @default.
- W3201504662 cites W2152770371 @default.
- W3201504662 cites W2238780918 @default.
- W3201504662 cites W2255216182 @default.
- W3201504662 cites W2256742438 @default.
- W3201504662 cites W2473118461 @default.
- W3201504662 cites W2548569529 @default.
- W3201504662 cites W2553773553 @default.
- W3201504662 cites W2743326951 @default.
- W3201504662 cites W2744018748 @default.
- W3201504662 cites W2772045429 @default.
- W3201504662 cites W2807024943 @default.
- W3201504662 cites W2890328386 @default.
- W3201504662 cites W2892986323 @default.
- W3201504662 cites W2899654810 @default.
- W3201504662 cites W2901328211 @default.
- W3201504662 cites W2909907659 @default.
- W3201504662 cites W2918043555 @default.
- W3201504662 cites W2951882399 @default.
- W3201504662 cites W2973046603 @default.
- W3201504662 cites W3025781942 @default.
- W3201504662 cites W3029496903 @default.
- W3201504662 cites W3083651411 @default.
- W3201504662 cites W639750498 @default.
- W3201504662 cites W2089213848 @default.
- W3201504662 doi "https://doi.org/10.1016/j.chroma.2021.462544" @default.
- W3201504662 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34543881" @default.
- W3201504662 hasPublicationYear "2021" @default.
- W3201504662 type Work @default.
- W3201504662 sameAs 3201504662 @default.
- W3201504662 citedByCount "6" @default.
- W3201504662 countsByYear W32015046622022 @default.
- W3201504662 crossrefType "journal-article" @default.
- W3201504662 hasAuthorship W3201504662A5000323275 @default.
- W3201504662 hasAuthorship W3201504662A5023990028 @default.
- W3201504662 hasAuthorship W3201504662A5041209710 @default.
- W3201504662 hasAuthorship W3201504662A5053447705 @default.
- W3201504662 hasAuthorship W3201504662A5091095745 @default.
- W3201504662 hasConcept C116569031 @default.
- W3201504662 hasConcept C170493617 @default.
- W3201504662 hasConcept C185592680 @default.
- W3201504662 hasConcept C41625074 @default.
- W3201504662 hasConcept C43617362 @default.
- W3201504662 hasConcept C55493867 @default.
- W3201504662 hasConceptScore W3201504662C116569031 @default.
- W3201504662 hasConceptScore W3201504662C170493617 @default.
- W3201504662 hasConceptScore W3201504662C185592680 @default.
- W3201504662 hasConceptScore W3201504662C41625074 @default.
- W3201504662 hasConceptScore W3201504662C43617362 @default.
- W3201504662 hasConceptScore W3201504662C55493867 @default.
- W3201504662 hasFunder F4320323993 @default.
- W3201504662 hasLocation W32015046621 @default.
- W3201504662 hasOpenAccess W3201504662 @default.
- W3201504662 hasPrimaryLocation W32015046621 @default.
- W3201504662 hasRelatedWork W2002032965 @default.
- W3201504662 hasRelatedWork W2011802865 @default.
- W3201504662 hasRelatedWork W2050940716 @default.
- W3201504662 hasRelatedWork W2053584692 @default.