Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201517488> ?p ?o ?g. }
- W3201517488 abstract "Nanoparticles can acquire a protein corona defining their biological identity. Corona functions were not yet considered for cell-derived extracellular vesicles (EVs). Here we demonstrate that nanosized EVs from therapy-grade human placental-expanded (PLX) stromal cells are surrounded by an imageable and functional protein corona when enriched with permissive technology. Scalable EV separation from cell-secreted soluble factors via tangential flow-filtration and subtractive tandem mass-tag proteomics revealed significant enrichment of predominantly immunomodulatory and proangiogenic proteins. Western blot, calcein-based flow cytometry, super-resolution and electron microscopy verified EV identity. PLX-EVs protected corona proteins from protease digestion. EVs significantly ameliorated human skin regeneration and angiogenesis in vivo, induced differential signaling in immune cells, and dose-dependently inhibited T cell proliferation in vitro. Corona removal by size-exclusion or ultracentrifugation abrogated angiogenesis. Re-establishing an artificial corona by cloaking EVs with defined proangiogenic proteins served as a proof-of-concept. Understanding EV corona formation will improve rational EV-inspired nanotherapy design." @default.
- W3201517488 created "2021-09-27" @default.
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- W3201517488 date "2019-10-17" @default.
- W3201517488 modified "2023-09-26" @default.
- W3201517488 title "A functional corona around extracellular vesicles enhances angiogenesis during skin regeneration and signals in immune cells" @default.
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- W3201517488 doi "https://doi.org/10.1101/808808" @default.
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