Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201549100> ?p ?o ?g. }
- W3201549100 endingPage "e123" @default.
- W3201549100 startingPage "e123" @default.
- W3201549100 abstract "Cyanobacteria are simple, efficient, genetically-tractable photosynthetic microorganisms which in principle represent ideal biocatalysts for CO2 capture and conversion. However, in practice, genetic instability and low productivity are key, linked problems in engineered cyanobacteria. We took a massively parallel approach, generating and characterising libraries of synthetic promoters and RBSs for the cyanobacterium Synechocystis sp. PCC 6803, and assembling a sparse combinatorial library of millions of metabolic pathway-encoding construct variants. Genetic instability was observed for some variants, which is expected when variants cause metabolic burden. Surprisingly however, in a single combinatorial round without iterative optimisation, 80% of variants chosen at random and cultured photoautotrophically over many generations accumulated the target terpenoid lycopene from atmospheric CO2, apparently overcoming genetic instability. This large-scale parallel metabolic engineering of cyanobacteria provides a new platform for development of genetically stable cyanobacterial biocatalysts for sustainable light-driven production of valuable products directly from CO2, avoiding fossil carbon or competition with food production." @default.
- W3201549100 created "2021-09-27" @default.
- W3201549100 creator A5047571061 @default.
- W3201549100 creator A5076196451 @default.
- W3201549100 creator A5080805114 @default.
- W3201549100 date "2021-09-23" @default.
- W3201549100 modified "2023-10-12" @default.
- W3201549100 title "Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria" @default.
- W3201549100 cites W108278810 @default.
- W3201549100 cites W1543999230 @default.
- W3201549100 cites W1761495431 @default.
- W3201549100 cites W1882036684 @default.
- W3201549100 cites W1963557779 @default.
- W3201549100 cites W1966222319 @default.
- W3201549100 cites W1966645118 @default.
- W3201549100 cites W1967382476 @default.
- W3201549100 cites W1969422740 @default.
- W3201549100 cites W1970422609 @default.
- W3201549100 cites W1970894726 @default.
- W3201549100 cites W1980511668 @default.
- W3201549100 cites W1988603978 @default.
- W3201549100 cites W1988785393 @default.
- W3201549100 cites W2005480013 @default.
- W3201549100 cites W2008566545 @default.
- W3201549100 cites W2015476615 @default.
- W3201549100 cites W2015914231 @default.
- W3201549100 cites W2018776590 @default.
- W3201549100 cites W2021286360 @default.
- W3201549100 cites W2022750609 @default.
- W3201549100 cites W2025095839 @default.
- W3201549100 cites W2025714086 @default.
- W3201549100 cites W2026174502 @default.
- W3201549100 cites W2026860581 @default.
- W3201549100 cites W2029022533 @default.
- W3201549100 cites W2047017952 @default.
- W3201549100 cites W2047360542 @default.
- W3201549100 cites W2057911693 @default.
- W3201549100 cites W2058582378 @default.
- W3201549100 cites W2058846641 @default.
- W3201549100 cites W2066820270 @default.
- W3201549100 cites W2088589945 @default.
- W3201549100 cites W2094807715 @default.
- W3201549100 cites W2096519866 @default.
- W3201549100 cites W2105248706 @default.
- W3201549100 cites W2112570909 @default.
- W3201549100 cites W2117741404 @default.
- W3201549100 cites W2120479418 @default.
- W3201549100 cites W2125723147 @default.
- W3201549100 cites W2127647062 @default.
- W3201549100 cites W2134069782 @default.
- W3201549100 cites W2140463038 @default.
- W3201549100 cites W2145741655 @default.
- W3201549100 cites W2152007804 @default.
- W3201549100 cites W2156663310 @default.
- W3201549100 cites W2165036418 @default.
- W3201549100 cites W2165821458 @default.
- W3201549100 cites W2216315517 @default.
- W3201549100 cites W2229824025 @default.
- W3201549100 cites W2234392716 @default.
- W3201549100 cites W2238393485 @default.
- W3201549100 cites W2295491699 @default.
- W3201549100 cites W2323328947 @default.
- W3201549100 cites W2324591890 @default.
- W3201549100 cites W2497615362 @default.
- W3201549100 cites W2524607249 @default.
- W3201549100 cites W2526975822 @default.
- W3201549100 cites W2527387613 @default.
- W3201549100 cites W2532859241 @default.
- W3201549100 cites W2541913771 @default.
- W3201549100 cites W2560641503 @default.
- W3201549100 cites W2568285054 @default.
- W3201549100 cites W2581678927 @default.
- W3201549100 cites W2750755657 @default.
- W3201549100 cites W2753288612 @default.
- W3201549100 cites W2761441559 @default.
- W3201549100 cites W2767407549 @default.
- W3201549100 cites W2773104035 @default.
- W3201549100 cites W2775803673 @default.
- W3201549100 cites W2779403649 @default.
- W3201549100 cites W2792863819 @default.
- W3201549100 cites W2793981825 @default.
- W3201549100 cites W2884155421 @default.
- W3201549100 cites W2884486416 @default.
- W3201549100 cites W2886035811 @default.
- W3201549100 cites W2900465289 @default.
- W3201549100 cites W2935704221 @default.
- W3201549100 cites W2952280526 @default.
- W3201549100 cites W2952578507 @default.
- W3201549100 cites W2967109725 @default.
- W3201549100 cites W2970864060 @default.
- W3201549100 cites W3000624298 @default.
- W3201549100 cites W3083196095 @default.
- W3201549100 cites W3125591848 @default.
- W3201549100 doi "https://doi.org/10.1093/nar/gkab791" @default.
- W3201549100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34554258" @default.
- W3201549100 hasPublicationYear "2021" @default.
- W3201549100 type Work @default.
- W3201549100 sameAs 3201549100 @default.