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• W3201568676 abstract "We studied the chemical and bactericidal properties of plasma-modified amino acid leucine treated in phosphate-buffered saline (PBS) using an atmospheric-pressure plasma jet operating with He/O2 gas. We determined the initial formation of hypochlorite by the reaction of plasma-supplied O atoms with chloride ions in PBS, followed by the subsequent chlorination of leucine. The principal chemical process involved the formation of leucine monochloramine and leucine dichloramine. The products of plasma-modified leucine further possessed post-discharge reactivity characterized by the decay of leucine chloramines to the secondary products, i.e., isovaleraldehyde, isovaleronitrile, and N-chloromethyl-3-butaldimine. Our study of the effects of leucine on the viability of E. coli bacteria in the plasma-treated PBS solution demonstrated time- and pH-dependent cytotoxic behavior, which were associated with the formation of different reaction products. At shorter treatment times, the addition of leucine hindered the bactericidal properties of plasma-treated PBS caused by the consumption of bactericidal hypochlorite in reaction with leucine and formation of non-bactericidal leucine monochloramine. Acidification to pH = 3 and longer plasma treatment substantially enhanced the bactericidal effects of leucine solutions, which were tentatively assigned to the formation of leucine dichloramine." @default.
• W3201568676 created "2021-09-27" @default.
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• W3201568676 date "2021-09-29" @default.
• W3201568676 modified "2023-10-18" @default.
• W3201568676 title "Leucine modifications by He/O₂ plasma treatment in phosphate-buffered saline: bactericidal effects and chemical characterization" @default.
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• W3201568676 doi "https://doi.org/10.1088/1361-6463/ac252e" @default.
• W3201568676 hasPublicationYear "2021" @default.
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