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• W3201619189 abstract "Abstract Receptors, transporters and ion channels are important targets for therapy development in neurological diseases including Alzheimeŕs disease, Parkinsońs disease, epilepsy, schizophrenia and major depression. Several receptors and ion channels identified by next generation sequencing may be involved in disease initiation and progression but their mechanistic role in pathogenesis is often poorly understood. Gene editing and in vivo imaging approaches will help to identify the molecular and functional role of these targets and the consequence of their regional dysfunction on whole brain level. Here, we combine CRISPR/Cas9 gene-editing with in vivo positron emission tomography (PET) and functional magnetic resonance imaging (fMRI) to investigate the direct link between genes, molecules, and the brain connectome. The extensive knowledge of the Slc18a2 gene encoding the vesicular monoamine transporter (VMAT2), involved in the storage and release of dopamine, makes it an excellent target for studying the gene networks relationships while structurally preserving neuronal integrity and function. We edited the Slc18a2 in the substantia nigra pars compacta of adult rats and used in vivo molecular imaging besides behavioral, histological, and biochemical assessments to characterize the CRISPR/Cas9-mediated VMAT2 knockdown. Simultaneous PET/fMRI was performed to investigate molecular and functional brain alterations. We found that stage-specific adaptations of brain functional connectivity follow the selective impairment of presynaptic dopamine storage and release. Our study reveals that recruiting different brain networks is an early response to the dopaminergic dysfunction preceding neuronal cell loss. Our combinatorial approach is a novel tool to investigate the impact of specific genes on brain molecular and functional dynamics which will help to develop tailored therapies for normalizing brain function. The method can easily be transferred to higher-order species allowing for a direct comparison of the molecular imaging findings." @default.
• W3201619189 created "2021-09-27" @default.
• W3201619189 creator A5018788118 @default.
• W3201619189 creator A5020683761 @default.
• W3201619189 creator A5034757315 @default.
• W3201619189 creator A5037569140 @default.
• W3201619189 creator A5052771182 @default.
• W3201619189 creator A5077765248 @default.
• W3201619189 creator A5083016310 @default.
• W3201619189 creator A5083192682 @default.
• W3201619189 date "2021-09-11" @default.
• W3201619189 modified "2023-09-23" @default.
• W3201619189 title "Combining CRISPR/Cas9 and brain imaging: from genes to molecules to networks" @default.
• W3201619189 cites W1575619372 @default.
• W3201619189 cites W1760829075 @default.
• W3201619189 cites W1829251706 @default.
• W3201619189 cites W1964642241 @default.
• W3201619189 cites W1964817667 @default.
• W3201619189 cites W1971032414 @default.
• W3201619189 cites W1972476556 @default.
• W3201619189 cites W1975312371 @default.
• W3201619189 cites W1977709885 @default.
• W3201619189 cites W1979119850 @default.
• W3201619189 cites W1979600308 @default.
• W3201619189 cites W1982843701 @default.
• W3201619189 cites W1987745724 @default.
• W3201619189 cites W1991691390 @default.
• W3201619189 cites W1996222810 @default.
• W3201619189 cites W1998241928 @default.
• W3201619189 cites W2001497035 @default.
• W3201619189 cites W2006715508 @default.
• W3201619189 cites W2009494091 @default.
• W3201619189 cites W2010722299 @default.
• W3201619189 cites W2011854027 @default.
• W3201619189 cites W2011913628 @default.
• W3201619189 cites W2014583795 @default.
• W3201619189 cites W2015641758 @default.
• W3201619189 cites W2018175707 @default.
• W3201619189 cites W2021645796 @default.
• W3201619189 cites W2027822127 @default.
• W3201619189 cites W2028590620 @default.
• W3201619189 cites W2034946478 @default.
• W3201619189 cites W2036892124 @default.
• W3201619189 cites W2039235656 @default.
• W3201619189 cites W2039696222 @default.
• W3201619189 cites W2042824095 @default.
• W3201619189 cites W2054230322 @default.
• W3201619189 cites W2056369385 @default.
• W3201619189 cites W2056512830 @default.
• W3201619189 cites W2056543335 @default.
• W3201619189 cites W2058784273 @default.
• W3201619189 cites W2059725343 @default.
• W3201619189 cites W2064815984 @default.
• W3201619189 cites W2066113689 @default.
• W3201619189 cites W2066667961 @default.
• W3201619189 cites W2066976976 @default.
• W3201619189 cites W2067147530 @default.
• W3201619189 cites W2070614858 @default.
• W3201619189 cites W2071714163 @default.
• W3201619189 cites W2072740414 @default.
• W3201619189 cites W2073695324 @default.
• W3201619189 cites W2075549646 @default.
• W3201619189 cites W2079975721 @default.
• W3201619189 cites W2085674390 @default.
• W3201619189 cites W2090450195 @default.
• W3201619189 cites W2097524044 @default.
• W3201619189 cites W2111352290 @default.
• W3201619189 cites W2111737291 @default.
• W3201619189 cites W2118830830 @default.
• W3201619189 cites W2121066313 @default.
• W3201619189 cites W2124384418 @default.
• W3201619189 cites W2134095170 @default.
• W3201619189 cites W2139495058 @default.
• W3201619189 cites W2139921727 @default.
• W3201619189 cites W2144762982 @default.
• W3201619189 cites W2146901380 @default.
• W3201619189 cites W2148822665 @default.
• W3201619189 cites W2150135821 @default.
• W3201619189 cites W2150872839 @default.
• W3201619189 cites W2152160545 @default.
• W3201619189 cites W2152226751 @default.
• W3201619189 cites W2153344788 @default.
• W3201619189 cites W2157461606 @default.
• W3201619189 cites W2158637234 @default.
• W3201619189 cites W2158678827 @default.
• W3201619189 cites W2161488472 @default.
• W3201619189 cites W2165793012 @default.
• W3201619189 cites W2165869860 @default.
• W3201619189 cites W2167822639 @default.
• W3201619189 cites W2170392605 @default.
• W3201619189 cites W2170978054 @default.
• W3201619189 cites W2299659552 @default.
• W3201619189 cites W2403977700 @default.
• W3201619189 cites W2409112471 @default.
• W3201619189 cites W2514315802 @default.
• W3201619189 cites W2547131243 @default.
• W3201619189 cites W2589144283 @default.
• W3201619189 cites W2606081028 @default.
• W3201619189 cites W2617052584 @default.
• W3201619189 cites W2735794863 @default.

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