Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201650239> ?p ?o ?g. }
- W3201650239 abstract "While extracellular matrix (ECM) mechanics strongly regulate stem cell commitment, the field′s mechanistic understanding of this phenomenon largely derives from simplified two–dimensional (2D) culture substrates. Here we found a three-dimensional (3D) matrix–specific mechanoresponsive mechanism for neural stem cell (NSC) differentiation. NSC lineage commitment in 3D is maximally stiffness-sensitive in the range of 0.1–1.2 kPa, a narrower and more brain-mimetic range than we had previously identified in 2D (0.75–75 kPa). Transcriptomics revealed stiffness-dependent upregulation of early growth response 1 (Egr1) in 3D but not in 2D. Egr1 knockdown enhanced neurogenesis in stiff ECMs by driving β–catenin nuclear localization and activity in 3D, but not in 2D. Mechanical modeling and experimental studies under osmotic pressure indicate that stiff 3D ECMs are likely to stimulate Egr1 via increases in confining stress during cell volumetric growth. To our knowledge, Egr1 represents the first 3D–specific stem cell mechanoregulatory factor." @default.
- W3201650239 created "2021-09-27" @default.
- W3201650239 creator A5001550074 @default.
- W3201650239 creator A5013729741 @default.
- W3201650239 creator A5015051062 @default.
- W3201650239 creator A5039621391 @default.
- W3201650239 creator A5048305189 @default.
- W3201650239 creator A5065625247 @default.
- W3201650239 date "2021-09-16" @default.
- W3201650239 modified "2023-10-16" @default.
- W3201650239 title "3D Microenvironment-Specific Mechanosensing Regulates Neural Stem Cell Lineage Commitment" @default.
- W3201650239 cites W1660095449 @default.
- W3201650239 cites W1870702458 @default.
- W3201650239 cites W1967188390 @default.
- W3201650239 cites W1967803088 @default.
- W3201650239 cites W1970570245 @default.
- W3201650239 cites W1975912195 @default.
- W3201650239 cites W1980151067 @default.
- W3201650239 cites W2003569663 @default.
- W3201650239 cites W2029702180 @default.
- W3201650239 cites W2032925606 @default.
- W3201650239 cites W2039700601 @default.
- W3201650239 cites W2053199336 @default.
- W3201650239 cites W2055036199 @default.
- W3201650239 cites W2055604817 @default.
- W3201650239 cites W2062301369 @default.
- W3201650239 cites W2063445979 @default.
- W3201650239 cites W2069264443 @default.
- W3201650239 cites W2075887668 @default.
- W3201650239 cites W2082773762 @default.
- W3201650239 cites W2089204174 @default.
- W3201650239 cites W2102554836 @default.
- W3201650239 cites W2104696158 @default.
- W3201650239 cites W2110947634 @default.
- W3201650239 cites W2113547540 @default.
- W3201650239 cites W2134208433 @default.
- W3201650239 cites W2144023435 @default.
- W3201650239 cites W2146337514 @default.
- W3201650239 cites W2160627858 @default.
- W3201650239 cites W2163149880 @default.
- W3201650239 cites W2167995816 @default.
- W3201650239 cites W2270498673 @default.
- W3201650239 cites W2342513881 @default.
- W3201650239 cites W2469522634 @default.
- W3201650239 cites W2499628912 @default.
- W3201650239 cites W2512410982 @default.
- W3201650239 cites W2514860072 @default.
- W3201650239 cites W2595255406 @default.
- W3201650239 cites W2611663512 @default.
- W3201650239 cites W2616779259 @default.
- W3201650239 cites W2737110471 @default.
- W3201650239 cites W2762429861 @default.
- W3201650239 cites W2765609903 @default.
- W3201650239 cites W2767569544 @default.
- W3201650239 cites W2775063014 @default.
- W3201650239 cites W2808195648 @default.
- W3201650239 cites W2890581378 @default.
- W3201650239 cites W2893129486 @default.
- W3201650239 cites W2896782101 @default.
- W3201650239 cites W2900958958 @default.
- W3201650239 cites W2920808209 @default.
- W3201650239 cites W2957594695 @default.
- W3201650239 cites W2969970501 @default.
- W3201650239 cites W2981899793 @default.
- W3201650239 cites W3014200134 @default.
- W3201650239 cites W3092393715 @default.
- W3201650239 doi "https://doi.org/10.1101/2021.09.15.460399" @default.
- W3201650239 hasPublicationYear "2021" @default.
- W3201650239 type Work @default.
- W3201650239 sameAs 3201650239 @default.
- W3201650239 citedByCount "0" @default.
- W3201650239 crossrefType "posted-content" @default.
- W3201650239 hasAuthorship W3201650239A5001550074 @default.
- W3201650239 hasAuthorship W3201650239A5013729741 @default.
- W3201650239 hasAuthorship W3201650239A5015051062 @default.
- W3201650239 hasAuthorship W3201650239A5039621391 @default.
- W3201650239 hasAuthorship W3201650239A5048305189 @default.
- W3201650239 hasAuthorship W3201650239A5065625247 @default.
- W3201650239 hasBestOaLocation W32016502391 @default.
- W3201650239 hasConcept C104317684 @default.
- W3201650239 hasConcept C127561419 @default.
- W3201650239 hasConcept C136834591 @default.
- W3201650239 hasConcept C148738053 @default.
- W3201650239 hasConcept C162746124 @default.
- W3201650239 hasConcept C169760540 @default.
- W3201650239 hasConcept C173396325 @default.
- W3201650239 hasConcept C189165786 @default.
- W3201650239 hasConcept C20875687 @default.
- W3201650239 hasConcept C28328180 @default.
- W3201650239 hasConcept C4746552 @default.
- W3201650239 hasConcept C54355233 @default.
- W3201650239 hasConcept C55493867 @default.
- W3201650239 hasConcept C81885089 @default.
- W3201650239 hasConcept C86803240 @default.
- W3201650239 hasConcept C95444343 @default.
- W3201650239 hasConceptScore W3201650239C104317684 @default.
- W3201650239 hasConceptScore W3201650239C127561419 @default.
- W3201650239 hasConceptScore W3201650239C136834591 @default.
- W3201650239 hasConceptScore W3201650239C148738053 @default.
- W3201650239 hasConceptScore W3201650239C162746124 @default.