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• W3201740374 abstract "Abstract Microfluidic lung‐on‐a‐chip systems are increasingly attractive tools for studying lung physiology and function because of their ability to accurately recapitulate spatiotemporal features of the airway tissue microenvironment including cellular organization, tissue architecture, and mechanical cues such as cyclic stretching and airflow. However, most lung‐on‐a‐chip devices to date rely on integrated design elements like membranes for airway cell culture, and focus mainly on enabling on‐chip monitoring and analysis while neglecting the need for off‐chip analysis. Here, an extractable floating liquid‐gel‐based organ‐on‐a‐chip for airway tissue modeling referred to as “E‐FLOAT” is described that is arrayable, scalable, and uniquely amenable to withstand physiologic airflow by microanchors. It is shown that E‐FLOAT can be combined with a custom airflow system that permits controlled injection of particulate matter for air pollution studies. Results show that airflow is critical to efficiently achieving physiologic mimicry of airway epithelium composition, tight junction expression, mucus production, and cilia formation on epithelial cells. It is also shown that E‐FLOAT allows standard on‐chip analysis while permitting complete sample extraction and off‐chip analysis via immunocytochemistry, microscopy, and histological sectioning and staining, thereby expanding the number and types of biological assays that can be used and questions that can be tackled." @default.
• W3201740374 created "2021-10-11" @default.
• W3201740374 creator A5001272422 @default.
• W3201740374 creator A5069304202 @default.
• W3201740374 date "2021-10-01" @default.
• W3201740374 modified "2023-10-17" @default.
• W3201740374 title "E‐FLOAT: Extractable Floating Liquid Gel‐Based Organ‐on‐a‐Chip for Airway Tissue Modeling under Airflow" @default.
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• W3201740374 doi "https://doi.org/10.1002/admt.202100828" @default.
• W3201740374 hasPublicationYear "2021" @default.
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