Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201746189> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W3201746189 abstract "Author(s): Lin, Victor | Advisor(s): Shaw, Peter; Feng, Gen-Sheng | Abstract: Age-related macular degeneration (AMD) is a major cause of central vision loss among the elderly. Genome-wide association studies (GWAS) have implicated polymorphisms in complement factor H (CFH) and high-temperature requirement A1 (HTRA1) in progression to advanced AMD. The complement factor H plays a key regulator role in complement system of the innate immune defense. CFH is also found to have high affinity binding with oxidized phospholipids (oxPLs), which shields oxPLs from inducing inflammation in the eye. On the other hand, the HTRA1 is a serine protease that is identified to be an extracellular matrix homeostasis regulator and TGF-β1 pathway inhibitor. Despite the extensive studies on the two proteins’ respective properties and involved pathways, the mechanism between the genetic risk factors and AMD disease pathology remains to be explored. My research project investigates the cellular interactions and molecular mechanisms of CFH and HTRA1 in elevated oxidative stress environment. In this study, recombinant CFH and HTRA1 variants were produced for the experiments. The inhibitory effect of CFH and HTRA1 in modulating the uptake of oxidized low-density lipoprotein (oxLDL) into the retinal pigment epithelium cells (RPE) is demonstrated by Oil Red O staining. The CFH risk variant was identified to exhibit competitive binding against HTRA1 interaction with lipoproteins via competitive ELISA. Furthermore, CD36 mRNA expression is found to be significantly heightened with the administration of HTRA1 or oxidative stress. However, the CD36 protein level analysis via immunocytochemistry and western blot demonstrates reduction of CD36 protein expression by proteolytic active HTRA1. We also found that CD36 protein expression is either preserved or enhanced with the addition of CFH variants, which we speculate to protect CD36 against proteolytic cleavage by HTRA1. This preliminary evidence suggests that CFH and HTRA1 have an antagonistic effect on inhibiting oxidized phospholipids uptake in RPE cells. This finding provides a new aspect of complement independent role of CFH in lipid metabolism which may be explored as a therapeutic treatment to AMD." @default.
- W3201746189 created "2021-10-11" @default.
- W3201746189 creator A5031644679 @default.
- W3201746189 date "2019-01-01" @default.
- W3201746189 modified "2023-09-27" @default.
- W3201746189 title "CFH and HTRA1 exhibit antagonistic effect on inhibiting oxidized phospholipids uptake in human retinal pigment epithelial cells" @default.
- W3201746189 hasPublicationYear "2019" @default.
- W3201746189 type Work @default.
- W3201746189 sameAs 3201746189 @default.
- W3201746189 citedByCount "0" @default.
- W3201746189 crossrefType "journal-article" @default.
- W3201746189 hasAuthorship W3201746189A5031644679 @default.
- W3201746189 hasConcept C104317684 @default.
- W3201746189 hasConcept C111684460 @default.
- W3201746189 hasConcept C118487528 @default.
- W3201746189 hasConcept C136449434 @default.
- W3201746189 hasConcept C14356644 @default.
- W3201746189 hasConcept C153911025 @default.
- W3201746189 hasConcept C179369057 @default.
- W3201746189 hasConcept C189446657 @default.
- W3201746189 hasConcept C203014093 @default.
- W3201746189 hasConcept C2776151105 @default.
- W3201746189 hasConcept C2776403814 @default.
- W3201746189 hasConcept C2776415932 @default.
- W3201746189 hasConcept C2778024200 @default.
- W3201746189 hasConcept C2779828298 @default.
- W3201746189 hasConcept C2780827179 @default.
- W3201746189 hasConcept C55493867 @default.
- W3201746189 hasConcept C71924100 @default.
- W3201746189 hasConcept C79196540 @default.
- W3201746189 hasConcept C86803240 @default.
- W3201746189 hasConcept C8891405 @default.
- W3201746189 hasConcept C95444343 @default.
- W3201746189 hasConceptScore W3201746189C104317684 @default.
- W3201746189 hasConceptScore W3201746189C111684460 @default.
- W3201746189 hasConceptScore W3201746189C118487528 @default.
- W3201746189 hasConceptScore W3201746189C136449434 @default.
- W3201746189 hasConceptScore W3201746189C14356644 @default.
- W3201746189 hasConceptScore W3201746189C153911025 @default.
- W3201746189 hasConceptScore W3201746189C179369057 @default.
- W3201746189 hasConceptScore W3201746189C189446657 @default.
- W3201746189 hasConceptScore W3201746189C203014093 @default.
- W3201746189 hasConceptScore W3201746189C2776151105 @default.
- W3201746189 hasConceptScore W3201746189C2776403814 @default.
- W3201746189 hasConceptScore W3201746189C2776415932 @default.
- W3201746189 hasConceptScore W3201746189C2778024200 @default.
- W3201746189 hasConceptScore W3201746189C2779828298 @default.
- W3201746189 hasConceptScore W3201746189C2780827179 @default.
- W3201746189 hasConceptScore W3201746189C55493867 @default.
- W3201746189 hasConceptScore W3201746189C71924100 @default.
- W3201746189 hasConceptScore W3201746189C79196540 @default.
- W3201746189 hasConceptScore W3201746189C86803240 @default.
- W3201746189 hasConceptScore W3201746189C8891405 @default.
- W3201746189 hasConceptScore W3201746189C95444343 @default.
- W3201746189 hasLocation W32017461891 @default.
- W3201746189 hasOpenAccess W3201746189 @default.
- W3201746189 hasPrimaryLocation W32017461891 @default.
- W3201746189 hasRelatedWork W137478356 @default.
- W3201746189 hasRelatedWork W1676904719 @default.
- W3201746189 hasRelatedWork W1985630427 @default.
- W3201746189 hasRelatedWork W2008189019 @default.
- W3201746189 hasRelatedWork W2049336947 @default.
- W3201746189 hasRelatedWork W2060934800 @default.
- W3201746189 hasRelatedWork W2065324814 @default.
- W3201746189 hasRelatedWork W2148045758 @default.
- W3201746189 hasRelatedWork W2156612446 @default.
- W3201746189 hasRelatedWork W2296704298 @default.
- W3201746189 hasRelatedWork W2469213726 @default.
- W3201746189 hasRelatedWork W2524411157 @default.
- W3201746189 hasRelatedWork W2601370107 @default.
- W3201746189 hasRelatedWork W2922348433 @default.
- W3201746189 hasRelatedWork W3008912381 @default.
- W3201746189 hasRelatedWork W3110505655 @default.
- W3201746189 hasRelatedWork W3138285868 @default.
- W3201746189 hasRelatedWork W3195476215 @default.
- W3201746189 hasRelatedWork W759473602 @default.
- W3201746189 hasRelatedWork W906829574 @default.
- W3201746189 isParatext "false" @default.
- W3201746189 isRetracted "false" @default.
- W3201746189 magId "3201746189" @default.
- W3201746189 workType "article" @default.