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- W3201746753 abstract "Mycoplasma is one of the smallest and simplest prokaryotes. Mycoplasma contamination during cell culture is widespread, and the use of contaminated cells compromises almost all aspects of cell physiology and consequently the results and conclusions from numerous experiments. In this study, we tried to eliminate the mtcoplasma from mtcoplasma contaminated cases during human embryonic stem cell(hESC) culture. hESC lines (SNUhES4, SNUhE31, H1) were cultured with three diffetent antimycoplasma antibiotics (Moxifloxacin+Gentamicin, Plasmocin, Plasmocure) for 4~5 passges, We observed morphologies, rete of attachment and differentiation of hESCs. Also, we confirmed relative treatment efficiency (eliminated, resistant, dead) of antimtcoplasma antibiotics. Three weeks after Plasmocure treatment, high attachment tate and mycoplasma free condition were well maintained. Relative treatment efficiency of Plasmocure showed eliminated 88.9%, resisrant 11.1%, dead 0%. Among the tested antimycoplasma antibiotics, Plasmocure was a most effective antimycoplasma antibiotic to eliminate mycoplasma from contaminated cell culture cases." @default.
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- W3201746753 date "2014-01-01" @default.
- W3201746753 modified "2023-09-26" @default.
- W3201746753 title "인간 배아줄기세포 배양에서 마이코플라즈마 오염 시 제거를 위한 연구" @default.
- W3201746753 hasPublicationYear "2014" @default.
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