FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3201801952> ?p ?o ?g. }

• W3201801952 abstract "Eimeria necatrix is one of the most pathogenic parasites, causing high mortality in chickens. Although its genome sequence has been published, the sequences and complete structures of its mRNA transcripts remain unclear, limiting exploration of novel biomarkers, drug targets and genetic functions in E. necatrix.Second-generation merozoites (MZ-2) of E. necatrix were collected using Percoll density gradients, and high-quality RNA was extracted from them. Single-molecule real-time (SMRT) sequencing and Illumina sequencing were combined to generate the transcripts of MZ-2. Combined with the SMRT sequencing data of sporozoites (SZ) collected in our previous study, the transcriptome and transcript structures of E. necatrix were studied.SMRT sequencing yielded 21,923 consensus isoforms in MZ-2. A total of 17,151 novel isoforms of known genes and 3918 isoforms of novel genes were successfully identified. We also identified 2752 (SZ) and 3255 (MZ-2) alternative splicing (AS) events, 1705 (SZ) and 1874 (MZ-2) genes with alternative polyadenylation (APA) sites, 4019 (SZ) and 2588 (MZ-2) fusion transcripts, 159 (SZ) and 84 (MZ-2) putative transcription factors (TFs) and 3581 (SZ) and 2039 (MZ-2) long non-coding RNAs (lncRNAs). To validate fusion transcripts, reverse transcription-PCR was performed on 16 candidates, with an accuracy reaching up to 87.5%. Sanger sequencing of the PCR products further confirmed the authenticity of chimeric transcripts. Comparative analysis of transcript structures revealed a total of 3710 consensus isoforms, 815 AS events, 1139 genes with APA sites, 20 putative TFs and 352 lncRNAs in both SZ and MZ-2.We obtained many long-read isoforms in E. necatrix SZ and MZ-2, from which a series of lncRNAs, AS events, APA events and fusion transcripts were identified. Information on TFs will improve understanding of transcriptional regulation, and fusion event data will greatly improve draft versions of gene models in E. necatrix. This information offers insights into the mechanisms governing the development of E. necatrix and will aid in the development of novel strategies for coccidiosis control." @default.
• W3201801952 created "2021-10-11" @default.
• W3201801952 creator A5006349365 @default.
• W3201801952 creator A5017298015 @default.
• W3201801952 creator A5022708781 @default.
• W3201801952 creator A5041125507 @default.
• W3201801952 creator A5047182876 @default.
• W3201801952 creator A5053596030 @default.
• W3201801952 creator A5065548055 @default.
• W3201801952 creator A5070281407 @default.
• W3201801952 date "2021-09-27" @default.
• W3201801952 modified "2023-10-17" @default.
• W3201801952 title "Full-length transcriptome analysis and identification of transcript structures in Eimeria necatrix from different developmental stages by single-molecule real-time sequencing" @default.
• W3201801952 cites W1551073541 @default.
• W3201801952 cites W1964410750 @default.
• W3201801952 cites W1969289645 @default.
• W3201801952 cites W1976175745 @default.
• W3201801952 cites W1987263704 @default.
• W3201801952 cites W1998422171 @default.
• W3201801952 cites W1999659528 @default.
• W3201801952 cites W2000564844 @default.
• W3201801952 cites W2033075853 @default.
• W3201801952 cites W2050071610 @default.
• W3201801952 cites W2054405028 @default.
• W3201801952 cites W2055760378 @default.
• W3201801952 cites W2061548680 @default.
• W3201801952 cites W2062771530 @default.
• W3201801952 cites W2064792211 @default.
• W3201801952 cites W2067790545 @default.
• W3201801952 cites W2072437217 @default.
• W3201801952 cites W2075320162 @default.
• W3201801952 cites W2077883112 @default.
• W3201801952 cites W2079983771 @default.
• W3201801952 cites W2090128221 @default.
• W3201801952 cites W2094671950 @default.
• W3201801952 cites W2096465161 @default.
• W3201801952 cites W2099738370 @default.
• W3201801952 cites W2099915768 @default.
• W3201801952 cites W2106678197 @default.
• W3201801952 cites W2108218546 @default.
• W3201801952 cites W2117787165 @default.
• W3201801952 cites W2128956445 @default.
• W3201801952 cites W2135553161 @default.
• W3201801952 cites W2135621733 @default.
• W3201801952 cites W2135743054 @default.
• W3201801952 cites W2143780453 @default.
• W3201801952 cites W2146041009 @default.
• W3201801952 cites W2147364153 @default.
• W3201801952 cites W2160177274 @default.
• W3201801952 cites W2162547088 @default.
• W3201801952 cites W2167432290 @default.
• W3201801952 cites W2195724570 @default.
• W3201801952 cites W2224056471 @default.
• W3201801952 cites W2337011385 @default.
• W3201801952 cites W2463761386 @default.
• W3201801952 cites W2516427586 @default.
• W3201801952 cites W2605929552 @default.
• W3201801952 cites W2612519109 @default.
• W3201801952 cites W2747721337 @default.
• W3201801952 cites W2752253975 @default.
• W3201801952 cites W2755732539 @default.
• W3201801952 cites W2774533158 @default.
• W3201801952 cites W2788588748 @default.
• W3201801952 cites W2788852505 @default.
• W3201801952 cites W2803607595 @default.
• W3201801952 cites W2803880697 @default.
• W3201801952 cites W2806507199 @default.
• W3201801952 cites W2889062795 @default.
• W3201801952 cites W2895607350 @default.
• W3201801952 cites W2907237883 @default.
• W3201801952 cites W2914952744 @default.
• W3201801952 cites W2922354271 @default.
• W3201801952 cites W2944018161 @default.
• W3201801952 cites W2945142887 @default.
• W3201801952 cites W2947358513 @default.
• W3201801952 cites W2948657373 @default.
• W3201801952 cites W2953929685 @default.
• W3201801952 cites W2964991885 @default.
• W3201801952 cites W2969999157 @default.
• W3201801952 cites W3011539660 @default.
• W3201801952 cites W3014518826 @default.
• W3201801952 cites W3087297477 @default.
• W3201801952 cites W3088296650 @default.
• W3201801952 cites W3171383293 @default.
• W3201801952 cites W362120134 @default.
• W3201801952 cites W958219903 @default.
• W3201801952 doi "https://doi.org/10.1186/s13071-021-05015-7" @default.
• W3201801952 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8474931" @default.
• W3201801952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34579769" @default.
• W3201801952 hasPublicationYear "2021" @default.
• W3201801952 type Work @default.
• W3201801952 sameAs 3201801952 @default.
• W3201801952 citedByCount "3" @default.
• W3201801952 countsByYear W32018019522022 @default.
• W3201801952 countsByYear W32018019522023 @default.
• W3201801952 crossrefType "journal-article" @default.
• W3201801952 hasAuthorship W3201801952A5006349365 @default.
• W3201801952 hasAuthorship W3201801952A5017298015 @default.
• W3201801952 hasAuthorship W3201801952A5022708781 @default.
• W3201801952 hasAuthorship W3201801952A5041125507 @default.

• next